Summary Topic 5 Hill Reaction

Hill reaction
Independent variable
light intensity reacting he chloroplasts
samples

S.I units Dependent variable - Amount of DLPIP reduced (colour
change
Control variables

Chloroplasts of he same
species
a
chopped spinach
↳ concentration of enzymes is constant


Amount
Jamb
chloroplasts/buffer
of
concentration is
solution added to each tube
uniform
>
-
,




Amount to each tube 10cmb will be
of DLPIP added added
·
-




rate to
Oxidation
uniform due concentration
-
is




·Amountlime bom tubes be
of will
fa e




compared
solution will be
Same pH used
buffer 0 added
I


to maintain pH of 7
activity
>
-

enzyme
.




affected by changes in active site .




Method :


&




-CutSpinachleaves
anda
, peste
in chilled isolation
medium

- Filter he liquid with a muslin cloth

-




transfer to
centrifuge tubes/pre coded

Centrifuge at
high speeds for
10 minutes
-




so
chloroplasts collect or he bottom as
a .
pellet