Summary
1st quarter microbiology 214 summary
- Course
- Microbiology 214
- Institution
- Stellenbosch University (SUN)
Handwritten notes for the first quarter of microbiology 214, including diagrams and formulae/calculations.
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Available practice questions
Flashcards
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Some examples from this set of practice questions
1.
Harmful micro-organisms that cause disease.
Answer: Pathogens
2.
Acellular parasites that require host cells.
Answer: Viruses
3.
Single-celled prokaryotes that contain peptidoglycan in their cell walls.
Answer: Bacteria
4.
Extremophiles with distinct rRNA sequences and no peptidoglycan in their cell walls.
Answer: Archaea
5.
Unicellular eukaryotes
Answer: Protists
6.
Multi- or unicellular eukaryotes
Answer: Fungi
7.
Give 3 similarities between archaea and eukaryotes.
Answer: Introns in genes, have a gene translation mechanism, no peptidoglycan in their cell walls.
8.
Give 3 similarities between archaea, eukaryotes and bacteria.
Answer: Presence of DNA, membrane-bound cytoplasm, presence of ribsomes.
9.
The theory that suggested organisms develop from non-living matter.
Answer: Spontaneous generation
10.
Disproved the theory of spontaneous generation for large animals by showing that maggots on decaying meat came from fly eggs.
Answer: Franscesco Redi
Flashcards
192 Flashcards
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Some examples from this set of practice questions
1.
Why is sampling important? (4)
Answer: Quality control, identification of potential industrial strains, disease diagnosis, answering fundamental research questions.
2.
Why are standard operating procedures important when it comes to sampling?
Answer: Ensures samples are the same, so results are valid and accurate.
3.
What is the advantage of using commercial sampling kits?
Answer: They are validated for certain uses and save time (however they are expensive).
4.
What should be considered when analysis requires intact nucleic acids?
Answer: Environmental conditions, DNA/RNA contamination, presence of proteases.
5.
What are the disadvantages of culture-dependent methods?
Answer: Unculturable microorganisms, labour intensive, time-consuming.
6.
What are the advantages of culture-independent (molecular) methods?
Answer: No culturing required, quick, can be used to detect byproducts.
7.
What are the disadvantages of culture-independent (molecular) methods?
Answer: Require special skills and complex extraction methods, expensive equipment.
8.
What are the three methods of enumeration?
Answer: 1. Dilution series and plating to determine CFUs. 2. Gridded membrane filter. 3. Ten-fold dilution series to determine the most probable number.
9.
What factors should be considered when choosing between culture-dependent and -independent methods?
Answer: Time available, cost, high- or low-throughput.
10.
What is complex media?
Answer: Media with an UNKNOWN chemical composition, providing the nutritional growth requirements for MOST microorganisms.
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