CLGT 5506 (CLGT5506)

This document covers various nucleic acid amplification techniques, including PCR, Ligase Chain Reaction, and signal amplification. It provides an in-depth look at the conditions necessary for successful amplification, including buffer composition, cycle parameters, and the role of additives like DMSO and glycerol. Ideal for anyone working with DNA amplification in molecular diagnostics or genetic research.

Focused on techniques for accurately quantifying DNA, this document explains both spectrophotometry and fluorimetry methods. It provides guidelines on using optical density at specific wavelengths to assess DNA purity and concentration, with additional insights into PCR-RFLP and enzymatic reactions. A must-have reference for researchers needing precise DNA quantification for experiments like NGS preparation.

This comprehensive guide details the steps for isolating high-quality human genomic DNA, including methods such as phenol/chloroform extraction and spin-column DNA isolation. It covers cell lysis, protein removal, DNA precipitation, and quality assessment using techniques like gel electrophoresis. This document is essential for lab professionals engaged in genetic research and DNA analysis.

This collection of notes provides an essential guide for professionals in molecular biology and genetic research. It includes detailed procedures on specimen procurement, highlighting best practices for collecting and handling biological samples such as blood, tissue, and prenatal specimens, with specific instructions for DNA, RNA, and ultrahigh molecular weight DNA preservation. The human genomic DNA isolation document outlines comprehensive methods for isolating high-quality DNA using techniqu...