ASCP Board Exam - Clinical Chemistry:
Questions With In-Depth Solutions
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Terms in this set (126)
Changes in concentration of an analyte based on
Diurnal variation
the time of the day (measured at day vs. night time)
What is increased in the ACTH, Cortisol, Iron
AM? (b/c of diurnal
variation)
adrenocorticotropic hormone- stimulates adrenal
ACTH role
cortex to secrete CORTISOL
How does stress affect Increase in ACTH, cortisol, and catecholamines
chemistry values?
Hormones made by adrenal glands during stress.
Catecholamines
Ex. dopamine, epinephrine, norepinephrine
Which analyte values are Ammonia, lactic acid, K, Mg, LD, glucose
affected if delay in
processing b/c
serum/plasma is not
separated in time?
Components of Light source- monochromator- cuvette-
Spectrophotometry (in photodetector- readout
order)
Fluorometry components Light source- primary monochromator- cuvette-
(in order) secondary monochromator- detector- readout
When is thin-layer Screening test for drugs of abuse in urine
chromatography
primarily used?
, When is high- Separation of thermolabile compounds
performance liquid
chromatography (HPLC)
used?
When is gas Separation of volatile compounds (therapeutic &
chromatography used? toxic drugs)
what is Glucose and major source of cellular energy. Levels decrease if
when does it decrease sample sits at RT.
What are the common Glucose oxidase & hexokinase= most common
methods to measure (hexokinase is more accurate)
glucose?
Is cholesterol a risk factor not by itself (must be analyzed with HDL & LDL)
for coronary artery
disease (CAD)?
HDL "good fat"- levels should be >60 mg/dL
"bad fat"- calculated via Friedwald formula
LDL Risk factor for coronary artery disease
levels should be <100 mg/dL
Main form of lipid storage- need fasting specimen
Triglycerides
levels should be <150 mg/dL
What method is used to Biuret method
measure total protein?
Biuret method depends Amount of PEPTIDE BONDS
on the presence of what?
- (detection of total
protein levels)
What plasma protein is albumin (synthesized in the liver)
the most abundant?
Albumin levels if Increased
dehydrated
Questions With In-Depth Solutions
Save
Terms in this set (126)
Changes in concentration of an analyte based on
Diurnal variation
the time of the day (measured at day vs. night time)
What is increased in the ACTH, Cortisol, Iron
AM? (b/c of diurnal
variation)
adrenocorticotropic hormone- stimulates adrenal
ACTH role
cortex to secrete CORTISOL
How does stress affect Increase in ACTH, cortisol, and catecholamines
chemistry values?
Hormones made by adrenal glands during stress.
Catecholamines
Ex. dopamine, epinephrine, norepinephrine
Which analyte values are Ammonia, lactic acid, K, Mg, LD, glucose
affected if delay in
processing b/c
serum/plasma is not
separated in time?
Components of Light source- monochromator- cuvette-
Spectrophotometry (in photodetector- readout
order)
Fluorometry components Light source- primary monochromator- cuvette-
(in order) secondary monochromator- detector- readout
When is thin-layer Screening test for drugs of abuse in urine
chromatography
primarily used?
, When is high- Separation of thermolabile compounds
performance liquid
chromatography (HPLC)
used?
When is gas Separation of volatile compounds (therapeutic &
chromatography used? toxic drugs)
what is Glucose and major source of cellular energy. Levels decrease if
when does it decrease sample sits at RT.
What are the common Glucose oxidase & hexokinase= most common
methods to measure (hexokinase is more accurate)
glucose?
Is cholesterol a risk factor not by itself (must be analyzed with HDL & LDL)
for coronary artery
disease (CAD)?
HDL "good fat"- levels should be >60 mg/dL
"bad fat"- calculated via Friedwald formula
LDL Risk factor for coronary artery disease
levels should be <100 mg/dL
Main form of lipid storage- need fasting specimen
Triglycerides
levels should be <150 mg/dL
What method is used to Biuret method
measure total protein?
Biuret method depends Amount of PEPTIDE BONDS
on the presence of what?
- (detection of total
protein levels)
What plasma protein is albumin (synthesized in the liver)
the most abundant?
Albumin levels if Increased
dehydrated
