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CH. 3 – Microscopy
3.1 Principles of Microscopy
EASY
1. In the history of microscopy, who is considered the “Father of Microbiology”?
a. Hans Jansen b. Zacharias Jansen C. Antonie van Leeuwenhoek d. Ernst Ruska
MEDIUM
2. Match the scientist with their contribution to the history of microscopy:
Scientist Contribution
Hans and Zacharias Jansen D a. developed electron microscope
Ernst Ruska A b. developed scanning tunneling microscope
Robert Hooke C c. First to use the term “cell”
Gerd Binning and Heinrich Roher B d. invented first compound microscope
EASY
3. What two features of a microscope are essential for a microbiologist?
A. magnification and resolution
b. magnification and oil immersion
c. resolution and oil immersion
d. objectives and oculars
MEDIUM
4. What is the definition of the term “magnification”?
a. ratio of the size of the bacterium to the size of the object
b. ratio of the size of the protozoan to the size of the objective
C. ratio of the size of the image to the size of the object
d. ratio of the size of the objective to the size of the microorganism
HARD
5. Which of the following best defines the term “resolution”?
a. the degree to which a microscope shows two images very far apart
b. the degree to which a microscope shows two or more cells as being distinct
c. the degree to which a microscope shows two or more cells as being close together
D. the degree to which a microscope shows two very close objects as being separate and apart
EASY
6. Match the part of the Compound Light Microscope with its purpose:
Microscope Part Purpose
Iris diaphragm D a. focuses light on specimen
Condenser A b. separately magnify specimen
Objective and Ocular B c. holds the specimen in place
Stage C d. controls light intensity via adjustable opening
, 2
EASY
7. What is the correct order of microscope parts from the base to the ocular?
A. base, illuminator, condenser lens, iris diaphragm, stage, objective lens, ocular lens
b. base, condenser lens, illuminator, iris diaphragm, objective lens, stage, ocular lens
c. base, iris diaphragm, stage, illuminator, condenser lens, objective lens, ocular lens
d. base, stage, condenser lens, illuminator, objective lens, stage, iris diaphragm, ocular lens
MEDIUM
8. What two things determine magnification of a microscope?
a. strength of objective X strength of ocular
B. degree of lens curvature and physics of how light refracts in its movement through different media
c. degree of lens curvature X strength of objective
d. degree of lens curvature X strength of objective X strength of ocular
HARD
9. What is the significance of the wavelength of light used for RP (resolving power)?
a. since blue, indigo and violet light have the shortest wavelengths, they influence the resolution or RP
b. since red, orange, and yellow have the longest wavelengths, they influence the resolution or RP
C. the resolution or RP of a microscope is directly related to which wavelength of light is utilized
d. the resolution or RP of a microscope is directly related to the number of wavelengths of light used
MEDIUM
10. What is meant by the “refractive index” of the medium?
a. resolution is affected by light that passes through a medium
b. a light passes through a medium it is referred to as refractive index
c. a direct proportion between refractive index and RP: greater the RI, then the better the RP and the
amount of detail that can be seen.
D. all of these are correct
EASY
11. What is the effect of using immersion oil plus oil immersion objective?
a. RP is increased because immersion oil has same RI of glass
b. Glass -> oil -> glass sandwich passes light directly into objective without losing light
c. increases both magnification and RP of microscope
d. magnification increases from 1000X to 4500X
E. answers a, b, and c are correct
3.2 Microscopy Used for Clinical Diagnosis
EASY
12. Match the type of microscope used in clinical settings with its principle description:
Microscope Description
Bright-field B a. fluorescent portions of specimen become visible
Dark-field C b. simplest, most common type
Fluorescence A c. bright image against dark background
CH. 3 – Microscopy
3.1 Principles of Microscopy
EASY
1. In the history of microscopy, who is considered the “Father of Microbiology”?
a. Hans Jansen b. Zacharias Jansen C. Antonie van Leeuwenhoek d. Ernst Ruska
MEDIUM
2. Match the scientist with their contribution to the history of microscopy:
Scientist Contribution
Hans and Zacharias Jansen D a. developed electron microscope
Ernst Ruska A b. developed scanning tunneling microscope
Robert Hooke C c. First to use the term “cell”
Gerd Binning and Heinrich Roher B d. invented first compound microscope
EASY
3. What two features of a microscope are essential for a microbiologist?
A. magnification and resolution
b. magnification and oil immersion
c. resolution and oil immersion
d. objectives and oculars
MEDIUM
4. What is the definition of the term “magnification”?
a. ratio of the size of the bacterium to the size of the object
b. ratio of the size of the protozoan to the size of the objective
C. ratio of the size of the image to the size of the object
d. ratio of the size of the objective to the size of the microorganism
HARD
5. Which of the following best defines the term “resolution”?
a. the degree to which a microscope shows two images very far apart
b. the degree to which a microscope shows two or more cells as being distinct
c. the degree to which a microscope shows two or more cells as being close together
D. the degree to which a microscope shows two very close objects as being separate and apart
EASY
6. Match the part of the Compound Light Microscope with its purpose:
Microscope Part Purpose
Iris diaphragm D a. focuses light on specimen
Condenser A b. separately magnify specimen
Objective and Ocular B c. holds the specimen in place
Stage C d. controls light intensity via adjustable opening
, 2
EASY
7. What is the correct order of microscope parts from the base to the ocular?
A. base, illuminator, condenser lens, iris diaphragm, stage, objective lens, ocular lens
b. base, condenser lens, illuminator, iris diaphragm, objective lens, stage, ocular lens
c. base, iris diaphragm, stage, illuminator, condenser lens, objective lens, ocular lens
d. base, stage, condenser lens, illuminator, objective lens, stage, iris diaphragm, ocular lens
MEDIUM
8. What two things determine magnification of a microscope?
a. strength of objective X strength of ocular
B. degree of lens curvature and physics of how light refracts in its movement through different media
c. degree of lens curvature X strength of objective
d. degree of lens curvature X strength of objective X strength of ocular
HARD
9. What is the significance of the wavelength of light used for RP (resolving power)?
a. since blue, indigo and violet light have the shortest wavelengths, they influence the resolution or RP
b. since red, orange, and yellow have the longest wavelengths, they influence the resolution or RP
C. the resolution or RP of a microscope is directly related to which wavelength of light is utilized
d. the resolution or RP of a microscope is directly related to the number of wavelengths of light used
MEDIUM
10. What is meant by the “refractive index” of the medium?
a. resolution is affected by light that passes through a medium
b. a light passes through a medium it is referred to as refractive index
c. a direct proportion between refractive index and RP: greater the RI, then the better the RP and the
amount of detail that can be seen.
D. all of these are correct
EASY
11. What is the effect of using immersion oil plus oil immersion objective?
a. RP is increased because immersion oil has same RI of glass
b. Glass -> oil -> glass sandwich passes light directly into objective without losing light
c. increases both magnification and RP of microscope
d. magnification increases from 1000X to 4500X
E. answers a, b, and c are correct
3.2 Microscopy Used for Clinical Diagnosis
EASY
12. Match the type of microscope used in clinical settings with its principle description:
Microscope Description
Bright-field B a. fluorescent portions of specimen become visible
Dark-field C b. simplest, most common type
Fluorescence A c. bright image against dark background
