BIOS251 OL, Week 4 Lab
Name:
OL Lab 4: Microscopy
Learning Objectives:
Understand different microscopy techniques and their limitations
Identify various cell types and cellular structures
Understand staining techniques
Understand coeliac disease and intestinal inflammation
Introduction:
Histology is an important tool of biology which helps us understand how
tissues are structured and how they function. This is helpful when we are
investigating diseased or malformed tissues.
In this simulation you will examine a chicken intestine sample that is
infected with a retrovirus and observe how the virus infects the
lymphocytes (the cells that provide immune protection) and how it
inhibits inflammation. Inflammation is a sign of tissue damage. You will
use different microscopic techniques to examine chicken intestine slide.
Try out the electron microscope
Electron microscopes can be used to visualize objects that are too small to
see when using a light microscope—for example the microvilli. You can zoom
in and out to observe different cellular structures such as mitochondria and
the cell. Using the 5X magnification, you will identify the villus, and then
proceed with higher magnifications to identify smooth muscle, extracellular
tissue, epithelial cells, Goblet cells and the nuclei.
Learn about fluorescence staining techniques
For example, by staining the DNA with DAPI, you can easily identify a cell’s
nucleus. In this part of the lab, you will examine a chicken intestine sample
that is infected with a retrovirus and observe how the virus infects the
lymphocytes and how it inhibits inflammation. The retrovirus can be further
developed as medicine for coeliac disease.
Name:
OL Lab 4: Microscopy
Learning Objectives:
Understand different microscopy techniques and their limitations
Identify various cell types and cellular structures
Understand staining techniques
Understand coeliac disease and intestinal inflammation
Introduction:
Histology is an important tool of biology which helps us understand how
tissues are structured and how they function. This is helpful when we are
investigating diseased or malformed tissues.
In this simulation you will examine a chicken intestine sample that is
infected with a retrovirus and observe how the virus infects the
lymphocytes (the cells that provide immune protection) and how it
inhibits inflammation. Inflammation is a sign of tissue damage. You will
use different microscopic techniques to examine chicken intestine slide.
Try out the electron microscope
Electron microscopes can be used to visualize objects that are too small to
see when using a light microscope—for example the microvilli. You can zoom
in and out to observe different cellular structures such as mitochondria and
the cell. Using the 5X magnification, you will identify the villus, and then
proceed with higher magnifications to identify smooth muscle, extracellular
tissue, epithelial cells, Goblet cells and the nuclei.
Learn about fluorescence staining techniques
For example, by staining the DNA with DAPI, you can easily identify a cell’s
nucleus. In this part of the lab, you will examine a chicken intestine sample
that is infected with a retrovirus and observe how the virus infects the
lymphocytes and how it inhibits inflammation. The retrovirus can be further
developed as medicine for coeliac disease.
