Bonus Questions on Molecular Diagnostics [100% PASS] /verified/

Bonus Questions on Molecular Diagnostics [100% PASS] /verified/ polymerase slippage *Ans* DNA polymerase can lose its place in a repetitive sequence tract, and either leaves out or add an inappropriate number of repeat units Imatinib *Ans* tyrosine kinase inhibitor (Gleevec) What is the rate of mutation per round of DNA replication? A. 1 in 1,000 base pairs B. 1 in 10,000 base pairs C. 1 in 1,000,000 base pairs D. 1 in 1,000,000,000 base pairs *Ans* D. 1 in 1,000,000,000 base pairs Which of the following is not a probe-labeling method? A. Labeling by random priming B. Labeling by nick translation C. End-labeling (using a terminal transferase enzyme) D. Labeling by hybrid capture *Ans* D. Labeling by hybrid capture 7. During DNA replication, the 3' -OH of the growing DNA chain attacks which phosphate of an incoming nucleotide? A. Alpha B. Beta C. Gamma D. Delta *Ans* B. Beta What drug is NOT metabolized by CYP2D6? A. Codeine B. Omeprazole C. Warfarin D. Escitalopram *Ans* C. Warfarin 15. Codons that code for the same amino acid are called: A. Synonyms B. Similar C. Degenerates D. Complements *Ans* A. Synonyms Amplification Refractory Mutation System *Ans* The amplification refractory mutation system (ARMS) is an amplification strategy in which a polymerase chain reaction (PCR) primer is designed in such a way that it is able to discriminate among templates that differ by a single nucleotide residue (1),2) ARMS has also been termed allele-specific PCR (3) or PCR amplification of specific alleles (PASA) (2). Thus, an ARMS primer can be designed to amplify a specific member of a multi-allelic system while remaining refractory to amplification of another allele that may doffer by as little as a single base from the former. The main advantage of ARMS