Kirby-Bauer Method Exam Questions with Complete Solutions

Natural antimicrobial agents produced by microorganisms. - Antibiotics Also called the disk diffusion test, is a valuable standard tool for measuring the effectiveness of antimicrobics against pathogenic microorganisms. - kirby bauer In the test, antimicrobic impregnated paper disks are placed on a plate that has been inoculated to form a bacterial lawn. The plates are incubated to allow growth of the bacteria and time for the agent to diffuse into the agar. As the drug moves through the agar, it establishes a concentration gradient. If the organism is susceptible to it, a clear ___ __ ______ will appear around the disk where growth has been inhibited. - zone of inhibition The size of this zone of inhibition depends on the sensitivity of the bacteria to the specific antimicrobial agent and the point at which the chemical's ____ ____ _____ is reached. - minimum inhibitory concentration (MIC) The zone may be produced as a result of killing the bacteria, in which case it is said to be ______. - bactericidal In other cases, the drug may only stop bacterial growth without killing them, in which case it is said to be _______. - bacteriostatic