Epithelium and Connective Tissue Practice TEST BANK
MIDTERM EXAM 2026/2027 AND HIGH YIELD
PRACTICE QUESTIONS COMPLETE ACCURATE EXAM
APPROVED QUESTIONS AND CORRECT VERIFIED
SOLUTIONS (100% CORRECT VERIFIED ANSWERS)
CURRENTLY UPDATED VERSION 2026 EDITION
Tissue preparation steps (histotechnique)
Fixation → Embedding → Section preparation → Staining.
Fixation (definition/purpose)
Preserves tissue structure.
Common fixative concentration used in routine histology
Usually 4% paraformaldehyde.
Other fixatives mentioned
Glutaraldehyde; alcohols (methanol).
Frozen preparations (when mentioned)
Sometimes tissue is processed as frozen preparations (cryosections).
,Embedding (definition/purpose)
Usually into paraffin; purpose is to enable cutting thin slices.
Microtome section thickness (normal histology)
5-10 µm.
Typical cell size range mentioned
~2-40 µm.
Ultramicrotome section types and thickness
Semithin: 0.5-1 µm; Ultrathin: 30-100 nm.
Cryotom / cryomicrotome
Used for frozen sections.
Where are sections placed after cutting
On glass slides.
Why staining is needed
Tissues are usually colorless; staining colors different tissue components.
Most frequent staining in practice
Hematoxylin-eosin (H&E).
, Hematoxylin (as an additional stain)
Used to show nuclei.
Stains where collagen is blue
Azan; Mallory; trichrome.
Stain where elastic fibers are brown
Orcein.
Toluidine blue (common use)
For semithin sections.
MIDTERM EXAM 2026/2027 AND HIGH YIELD
PRACTICE QUESTIONS COMPLETE ACCURATE EXAM
APPROVED QUESTIONS AND CORRECT VERIFIED
SOLUTIONS (100% CORRECT VERIFIED ANSWERS)
CURRENTLY UPDATED VERSION 2026 EDITION
Tissue preparation steps (histotechnique)
Fixation → Embedding → Section preparation → Staining.
Fixation (definition/purpose)
Preserves tissue structure.
Common fixative concentration used in routine histology
Usually 4% paraformaldehyde.
Other fixatives mentioned
Glutaraldehyde; alcohols (methanol).
Frozen preparations (when mentioned)
Sometimes tissue is processed as frozen preparations (cryosections).
,Embedding (definition/purpose)
Usually into paraffin; purpose is to enable cutting thin slices.
Microtome section thickness (normal histology)
5-10 µm.
Typical cell size range mentioned
~2-40 µm.
Ultramicrotome section types and thickness
Semithin: 0.5-1 µm; Ultrathin: 30-100 nm.
Cryotom / cryomicrotome
Used for frozen sections.
Where are sections placed after cutting
On glass slides.
Why staining is needed
Tissues are usually colorless; staining colors different tissue components.
Most frequent staining in practice
Hematoxylin-eosin (H&E).
, Hematoxylin (as an additional stain)
Used to show nuclei.
Stains where collagen is blue
Azan; Mallory; trichrome.
Stain where elastic fibers are brown
Orcein.
Toluidine blue (common use)
For semithin sections.
