Learning | 100 DNA/RNA, Replication, Transcription & Translation
Questions with Answers (2026) LATEST UPDATED THIS YEAR
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Overview
Prepare for the CHEM 210 Biochemistry Module 6 exam (Portage Learning) with this 100-question
multiple-choice practice test covering nucleic acid structure, DNA replication, transcription, RNA
processing, translation, gene regulation, and mutations. Each question includes the correct answer and a
concise, biochemistry-focused rationale to reinforce key concepts for college biochemistry, nursing,
pre-med, and allied health students in 2026. Ideal for module exam review, final exam prep, and
MCAT/NCLEX foundation building.
CHEM 210 Biochemistry – Module 6 practice exam aligned with typical Portage
Learning Module 6 content (nucleic acids, DNA/RNA structure, replication, transcription,
translation, and basic gene expression concepts).
SECTION 1 – Nucleic Acid Structure and Components (Q1–20)
Q1. DNA is primarily built from which of the following monomers?
A. Amino acids
B. Nucleotides
C. Fatty acids
D. Monosaccharides
B. Nucleotides
DNA polymers are formed from nucleotide subunits.
Q2. Which component is NOT part of a nucleotide?
,A. Phosphate group
B. Pentose sugar
C. Nitrogenous base
D. Amino group
D. Amino group
Nucleotides contain phosphate, sugar, and base, not a free amino group.
Q3. In DNA, the sugar component of each nucleotide is:
A. Ribose
B. Deoxyribose
C. Glucose
D. Fructose
B. Deoxyribose
DNA contains deoxyribose; RNA contains ribose.
Q4. Which nitrogenous base is found in DNA but NOT in RNA?
A. Adenine
B. Guanine
C. Thymine
D. Cytosine
C. Thymine
Thymine is replaced by uracil in RNA.
,Q5. In RNA, which base replaces thymine?
A. Adenine
B. Guanine
C. Uracil
D. Cytosine
C. Uracil
Uracil pairs with adenine in RNA.
Q6. The backbone of a DNA strand consists of alternating:
A. Sugar and phosphate groups
B. Sugar and nitrogenous bases
C. Phosphate and nitrogenous bases
D. Amino acids and sugars
A. Sugar and phosphate groups
The sugar-phosphate backbone supports the bases.
Q7. Complementary base pairing in DNA is primarily stabilized by:
A. Covalent bonds
B. Hydrogen bonds
C. Disulfide bonds
D. Peptide bonds
B. Hydrogen bonds
Hydrogen bonds hold complementary bases together.
, Q8. How many hydrogen bonds form between adenine and thymine in DNA?
A. One
B. Two
C. Three
D. Four
B. Two
A–T base pairs are held by two hydrogen bonds.
Q9. How many hydrogen bonds form between guanine and cytosine in DNA?
A. One
B. Two
C. Three
D. Four
C. Three
G–C base pairs are held by three hydrogen bonds.
Q10. Which statement correctly describes the orientation of DNA strands in the double
helix?
A. Both strands run 5′→3′ in the same direction
B. Strands are antiparallel, one 5′→3′ and the other 3′→5′
C. Strands are parallel and both 3′→5′
D. Strands have no defined direction