MLT ASCP LATEST 2026 EXAM QUESTIONS AND ANSWERS
RATED A+
✔✔C;
Protein in urine can be confirmed using sulfosalicylic acid (SSA) precipitation. The SSA
reagent is added to a small volume of urine. Acidification causes precipitation of protein
in the sample, which is subjectively graded as trace, 1+, 2+, 3+ or 4+. SSA reaction will
detect albumin, globulins, and Bence-Jones proteins. - ✔✔Which of the following would
be the most appropriate method to confirm a positive protein from a urine dipstick:
A. Immunoelectropheresis
B. Heat precipitation
C. Sulfosalicylic acid precipitation
D. Protein electrophoresis
✔✔C;
Patients who develop severe sepsis or septic shock commonly have increased plasma
lactic acid values. - ✔✔Patients who develop severe sepsis or septic shock commonly
have __________ plasma lactic acid values.
A. Decreased
B. Normal
C. Increased
✔✔B;
DAT ( Direct Antiglobulin Test ). - ✔✔When AHG or Coombs serum is used to
demonstrate that red cells are antibody coated in vivo, the procedure is termed:
A. Indirect technique
B. Direct technique
C. Hemagglutination technique
D. Hemolysis technique
✔✔D;
RAST tests, or Radioallergosorbent tests, are used to screen for an allergy to a specific
substance or substances if a person presents with allergy-like symptoms. - ✔✔The
assay which is most helpful in identifying specific allergens is:
A. Complement fixation
B. C-reactive proteins
C. RIA
D. RAST
✔✔B;
,Though it may not be required, TDM should still be used to confirm adequate dosing.
Genotyping does not make TDM redundant.
A PM will metabolize the drug more slowly and therefore will need lower doses.
CYP2D6 metabolizes many different drugs; it is not associated with just one class of
drugs. Anytime a drug is taken that competes for the same metabolizing enzyme as
another drug, there is potential for the concentrations of both drugs to be increased. -
✔✔A patient has been characterized as a CYP2D6 poor metabolizer (PM) after
genotyping. Which of the following statements is not true?
A. The patient will likely need lower doses of CYP2D6-metabolized drugs.
B. The patient is less likely to require therapeutic drug monitoring (TDM) since the
genotype is known.
C. CYP2D6 metabolizes many drugs, and so attention must be given to the doses of
drugs from different classes.
✔✔C;
One method of calculating a glomerular filtration rate is using creatinine and urine
volume to determine creatinine clearance. The equation is as follows:
Creatinine Clearance = (urine creatinine X urine flow rate) / plasma creatinine; where
urine flow rate = volume in mL /24 hours x h/60 min)
In this case = creatinine clearance = 124 X (2200/24 x hour/60) / 2 = 94.7 or 95 ml/min -
✔✔What is the glomerular filtration rate for a patient with a serum creatinine of 2 mg/dL,
if the urine creatinine was 124 mg/dL and the urine volume was 2.2 L/24 hrs?
A. 9.5 mL/min
B. 13.6 mL/min
C. 95 mL/min
D. 136 mL/min
E. 1.36 mL/min
✔✔B;
Listeria are non-capsulated, non-acid-fast, ß-hemolytic, gram-positive bacilli that have a
characteristic tumbling motility. They are found primarily in the environment and in the
human GI tract. Since L. monocytogenes multiplies intracellularly, control of listeriosis
requires cell-mediated immunity; thus, immunocompromised patients (such as
newborns) are at high risk. - ✔✔Which bacterial species is a common agent of neonatal
bacteremia?
A. Streptococcus pyogenes
B. Listeria monocytogenes
C. Staphylococcus aureus
D. Enterococcus faecium
, ✔✔D;
Platelets do not circulate in inactivated, spiny forms. The spiny, sticky form of the
platelet is initiated once the platelets become activated in response to blood vessel
damage. - ✔✔Which of the following is not true in terms of platelet characteristics?
A. Produced in the bone marrow by megakaryocytes.
B. Possess an inherent sticky property that aids in adhesion and aggregation.
C. Have a lifespan of roughly 9-12 days.
D. Are normally found circulating in their inactive, spiny form.
✔✔B;
HLA-DR is a MHC class II(major histocompatibility complex) surface receptor on the
cellular surface which is encoded by the human leukocyte antigen complex, or HLA. -
✔✔DR antigens are found in which of the following systems:
A. Kell system
B. HLA system
C. Duffy system
D. ABO system
✔✔B;
Serological diagnosis of active or recent infection generally requires the demonstration
of IgM antibody, or the demonstration of a fourfold rise in the titer of specific IgG
antibody. - ✔✔Which of the following would be considered most significant as it relates
to serological testing:
A. Presence of an antibody titer is generally diagnostic
B. Rise of antibody titers is diagnostic
C. Concentration of antibody is diagnostic
D. Cross reactivity is not significant
✔✔D;
Although cysteine-blood agar was traditionally used, F. tularensis will also grow on
commercially available Thayer-Martin and chocolate agar which have been enriched
with supplemental nutrients. - ✔✔Which of the following media would you use to isolate
Francisella tularensis:
A. Sheep-blood agar
B. Lowenstein-Jensen media
C. Bordet-Gengou media
D. Cysteine-blood agar
✔✔A, C, & D
The structures involved in the production of semen include the prostate, the seminal
vesicles, and the bulbourethral gland, along with the testes and epididymis.
RATED A+
✔✔C;
Protein in urine can be confirmed using sulfosalicylic acid (SSA) precipitation. The SSA
reagent is added to a small volume of urine. Acidification causes precipitation of protein
in the sample, which is subjectively graded as trace, 1+, 2+, 3+ or 4+. SSA reaction will
detect albumin, globulins, and Bence-Jones proteins. - ✔✔Which of the following would
be the most appropriate method to confirm a positive protein from a urine dipstick:
A. Immunoelectropheresis
B. Heat precipitation
C. Sulfosalicylic acid precipitation
D. Protein electrophoresis
✔✔C;
Patients who develop severe sepsis or septic shock commonly have increased plasma
lactic acid values. - ✔✔Patients who develop severe sepsis or septic shock commonly
have __________ plasma lactic acid values.
A. Decreased
B. Normal
C. Increased
✔✔B;
DAT ( Direct Antiglobulin Test ). - ✔✔When AHG or Coombs serum is used to
demonstrate that red cells are antibody coated in vivo, the procedure is termed:
A. Indirect technique
B. Direct technique
C. Hemagglutination technique
D. Hemolysis technique
✔✔D;
RAST tests, or Radioallergosorbent tests, are used to screen for an allergy to a specific
substance or substances if a person presents with allergy-like symptoms. - ✔✔The
assay which is most helpful in identifying specific allergens is:
A. Complement fixation
B. C-reactive proteins
C. RIA
D. RAST
✔✔B;
,Though it may not be required, TDM should still be used to confirm adequate dosing.
Genotyping does not make TDM redundant.
A PM will metabolize the drug more slowly and therefore will need lower doses.
CYP2D6 metabolizes many different drugs; it is not associated with just one class of
drugs. Anytime a drug is taken that competes for the same metabolizing enzyme as
another drug, there is potential for the concentrations of both drugs to be increased. -
✔✔A patient has been characterized as a CYP2D6 poor metabolizer (PM) after
genotyping. Which of the following statements is not true?
A. The patient will likely need lower doses of CYP2D6-metabolized drugs.
B. The patient is less likely to require therapeutic drug monitoring (TDM) since the
genotype is known.
C. CYP2D6 metabolizes many drugs, and so attention must be given to the doses of
drugs from different classes.
✔✔C;
One method of calculating a glomerular filtration rate is using creatinine and urine
volume to determine creatinine clearance. The equation is as follows:
Creatinine Clearance = (urine creatinine X urine flow rate) / plasma creatinine; where
urine flow rate = volume in mL /24 hours x h/60 min)
In this case = creatinine clearance = 124 X (2200/24 x hour/60) / 2 = 94.7 or 95 ml/min -
✔✔What is the glomerular filtration rate for a patient with a serum creatinine of 2 mg/dL,
if the urine creatinine was 124 mg/dL and the urine volume was 2.2 L/24 hrs?
A. 9.5 mL/min
B. 13.6 mL/min
C. 95 mL/min
D. 136 mL/min
E. 1.36 mL/min
✔✔B;
Listeria are non-capsulated, non-acid-fast, ß-hemolytic, gram-positive bacilli that have a
characteristic tumbling motility. They are found primarily in the environment and in the
human GI tract. Since L. monocytogenes multiplies intracellularly, control of listeriosis
requires cell-mediated immunity; thus, immunocompromised patients (such as
newborns) are at high risk. - ✔✔Which bacterial species is a common agent of neonatal
bacteremia?
A. Streptococcus pyogenes
B. Listeria monocytogenes
C. Staphylococcus aureus
D. Enterococcus faecium
, ✔✔D;
Platelets do not circulate in inactivated, spiny forms. The spiny, sticky form of the
platelet is initiated once the platelets become activated in response to blood vessel
damage. - ✔✔Which of the following is not true in terms of platelet characteristics?
A. Produced in the bone marrow by megakaryocytes.
B. Possess an inherent sticky property that aids in adhesion and aggregation.
C. Have a lifespan of roughly 9-12 days.
D. Are normally found circulating in their inactive, spiny form.
✔✔B;
HLA-DR is a MHC class II(major histocompatibility complex) surface receptor on the
cellular surface which is encoded by the human leukocyte antigen complex, or HLA. -
✔✔DR antigens are found in which of the following systems:
A. Kell system
B. HLA system
C. Duffy system
D. ABO system
✔✔B;
Serological diagnosis of active or recent infection generally requires the demonstration
of IgM antibody, or the demonstration of a fourfold rise in the titer of specific IgG
antibody. - ✔✔Which of the following would be considered most significant as it relates
to serological testing:
A. Presence of an antibody titer is generally diagnostic
B. Rise of antibody titers is diagnostic
C. Concentration of antibody is diagnostic
D. Cross reactivity is not significant
✔✔D;
Although cysteine-blood agar was traditionally used, F. tularensis will also grow on
commercially available Thayer-Martin and chocolate agar which have been enriched
with supplemental nutrients. - ✔✔Which of the following media would you use to isolate
Francisella tularensis:
A. Sheep-blood agar
B. Lowenstein-Jensen media
C. Bordet-Gengou media
D. Cysteine-blood agar
✔✔A, C, & D
The structures involved in the production of semen include the prostate, the seminal
vesicles, and the bulbourethral gland, along with the testes and epididymis.
