CTBS Exam 2025–2026 Accurate Real Exam
Questions and Verified Correct Answers
Which of the following transplants is most commonly associated with spread of prion
related disease?
a. Skin
b. Dura Mater
c. Bone
d. Blood Transfusion - answer>>>b. Dura Mater
Which of the following statements regarding rewarming of skin is NOT true?
a. A major goal is the maintenance of tissue viability
b. The temp of the rewarming bath should be 10-37 deg
c. It should be removed from it's package prior to rewarming
d. Significant structural damage can occur during rewarming - answer>>>c. It should
be removed from it's package prior to rewarming
Which of the following is NOT a true statement regarding shipment of skin to the
processing center:
a. The volume of transport solution must completely cover skin
b. The courier needs to be certified
c. The skin transportation container must be transported at wet ice temp
d. The skin transportation container must be fluid tight - answer>>>b. The courier
needs to be certified
Processing of skin that is to be frozen/cryopreserved must be initiated within days of
retrieval, provided the skin is placed in tissue storage media which is replaced every
hrs.
a. 5 days/48hrs
b. 10 days/72 hrs
c. 15 days/24 hrs
d. 3 days/24 hrs - answer>>>b. 10 days/72 hrs
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,The physical examination of the donor must include a documented assessment of factors
that may affect the quality and quantity of skin retrieved:
a. On every donor
b. Only if the family requests it
c. Only if you don't feel like recovering skin
d. Only when there is obvious trauma to skin - answer>>>a. On every donor
Frozen or cryopreserved skin must be stored at temp of or cooler
a. -20 degrees C
b. -10 degrees C
c. -5 degrees C
d. -40 degrees C - answer>>>d. -40 degrees C
Skin may be stored refrigerated for no longer than days
a. 12
b. 5
c. 10
d. 14 - answer>>>d. 14
The stratum corneum, stratum lucidum and stratum granulosum are all found in what
layer of the skin?
a. Dermis
b. Epidermis
c. Sweat Gland
d. Fascia - answer>>>b. Epidermis
An exceptional release of the skin requires written approval of the:
a. President/CEO
b. Donor Family
c. Trained Requestor
d. Medical Director - answer>>>d. Medical Director
Skin damaged by scraping or rubbing:
a. Bruise
b. Cut
c. Abrasion
d. Avulsion - answer>>>c. Abrasion
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,Small, dark, sometimes raised congenital growth on the human skin, sometimes with a
hair or hairs growing from it:
a. Wart
b. Mole, Nevus
c. Pimple
d. Birthmark - answer>>>b. Mole, Nevus
A swelling on the skin that contains fluid:
a. Contusion
b. Nevus
c. Mole
d. Blister - answer>>>d. Blister
On average adult skin covers square feet
a. 40
b. 19
c. 20
d. 15 - answer>>>b. 19
Cold ischemic time for vascular tissue is defined as:
a. The time interval from systole to subjecting cardiac tissue to cold rinse (or transport)
solution at retrieval
b. The time interval from subjecting vascular tissue to transport solution and wet ice temp
at retrieval to the beginning of disinfection
c. The time interval from systole to subjecting vascular tissue to transport solution and
wet ice temp at retrieval.
d. The time interval from subjecting cardiac tissue to cold rinse (or transport) solution at
retrieval to the beginning of disinfection - answer>>>d. The time interval from
subjecting cardiac tissue to cold rinse (or transport) solution at the retrieval to the
beginning of disinfection
Which of the following are NOT considered to be pathogenic, highly virulent
microorganisms that result in skeletal tissue discard:
a. Sabouraud's Dextrose Agar
b. Staphylococcus Aureus
c. Enterococcus Sp
d. Gram Negative Bacillis
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, e. Strepococcus Pyogenes
f. Fungi (Yeast/Molds) - answer>>>a. Sabouraud's Dextrose Agar
To prevent additional warm ischemia and potential cellular or matrix damage caused by
temp cycling, methods shall be employed that maintain the tissue or solutions above 1-10
deg for longer than:
a. 24hrs
b. 2 hrs
c. 1hr
d. 48hrs - answer>>>b. 2 hrs
Residual moisture analysis shall not exceed % of initial weight by nuclear magnetic
resonance spectrometry:
a. 8%
b. 6%
c. 2%
d. 10% - answer>>>a. 8%
What is the storage conditions temp for commonly transplanted human tissue: cardiac
and vascular:
a. 1-10 degrees C
b. -100 degrees C
c. 10-10 degrees C
d. -20to 4-10 degrees C - answer>>>b. -100 degrees C
Processing, which includes dissection and packaging, of cardiac and vascular tissue
shall be preformed in a certified and qualified Class ( or cleaner) laminar flow:
a. 1000
b. 500
c. 100
d. 200 - answer>>>c. 100
To prevent additional warm ischemia and potential cellular or matrix damage caused by
temp cycling, methods shall be employed that maintain the tissue and solutions:
a. 1-40 degrees
C b.1-10
degrees C
c. -1- -5 degrees C
d. 5 -10 degrees C - answer>>>b.1-10 degrees C
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Questions and Verified Correct Answers
Which of the following transplants is most commonly associated with spread of prion
related disease?
a. Skin
b. Dura Mater
c. Bone
d. Blood Transfusion - answer>>>b. Dura Mater
Which of the following statements regarding rewarming of skin is NOT true?
a. A major goal is the maintenance of tissue viability
b. The temp of the rewarming bath should be 10-37 deg
c. It should be removed from it's package prior to rewarming
d. Significant structural damage can occur during rewarming - answer>>>c. It should
be removed from it's package prior to rewarming
Which of the following is NOT a true statement regarding shipment of skin to the
processing center:
a. The volume of transport solution must completely cover skin
b. The courier needs to be certified
c. The skin transportation container must be transported at wet ice temp
d. The skin transportation container must be fluid tight - answer>>>b. The courier
needs to be certified
Processing of skin that is to be frozen/cryopreserved must be initiated within days of
retrieval, provided the skin is placed in tissue storage media which is replaced every
hrs.
a. 5 days/48hrs
b. 10 days/72 hrs
c. 15 days/24 hrs
d. 3 days/24 hrs - answer>>>b. 10 days/72 hrs
1|Page
,The physical examination of the donor must include a documented assessment of factors
that may affect the quality and quantity of skin retrieved:
a. On every donor
b. Only if the family requests it
c. Only if you don't feel like recovering skin
d. Only when there is obvious trauma to skin - answer>>>a. On every donor
Frozen or cryopreserved skin must be stored at temp of or cooler
a. -20 degrees C
b. -10 degrees C
c. -5 degrees C
d. -40 degrees C - answer>>>d. -40 degrees C
Skin may be stored refrigerated for no longer than days
a. 12
b. 5
c. 10
d. 14 - answer>>>d. 14
The stratum corneum, stratum lucidum and stratum granulosum are all found in what
layer of the skin?
a. Dermis
b. Epidermis
c. Sweat Gland
d. Fascia - answer>>>b. Epidermis
An exceptional release of the skin requires written approval of the:
a. President/CEO
b. Donor Family
c. Trained Requestor
d. Medical Director - answer>>>d. Medical Director
Skin damaged by scraping or rubbing:
a. Bruise
b. Cut
c. Abrasion
d. Avulsion - answer>>>c. Abrasion
2|Page
,Small, dark, sometimes raised congenital growth on the human skin, sometimes with a
hair or hairs growing from it:
a. Wart
b. Mole, Nevus
c. Pimple
d. Birthmark - answer>>>b. Mole, Nevus
A swelling on the skin that contains fluid:
a. Contusion
b. Nevus
c. Mole
d. Blister - answer>>>d. Blister
On average adult skin covers square feet
a. 40
b. 19
c. 20
d. 15 - answer>>>b. 19
Cold ischemic time for vascular tissue is defined as:
a. The time interval from systole to subjecting cardiac tissue to cold rinse (or transport)
solution at retrieval
b. The time interval from subjecting vascular tissue to transport solution and wet ice temp
at retrieval to the beginning of disinfection
c. The time interval from systole to subjecting vascular tissue to transport solution and
wet ice temp at retrieval.
d. The time interval from subjecting cardiac tissue to cold rinse (or transport) solution at
retrieval to the beginning of disinfection - answer>>>d. The time interval from
subjecting cardiac tissue to cold rinse (or transport) solution at the retrieval to the
beginning of disinfection
Which of the following are NOT considered to be pathogenic, highly virulent
microorganisms that result in skeletal tissue discard:
a. Sabouraud's Dextrose Agar
b. Staphylococcus Aureus
c. Enterococcus Sp
d. Gram Negative Bacillis
3|Page
, e. Strepococcus Pyogenes
f. Fungi (Yeast/Molds) - answer>>>a. Sabouraud's Dextrose Agar
To prevent additional warm ischemia and potential cellular or matrix damage caused by
temp cycling, methods shall be employed that maintain the tissue or solutions above 1-10
deg for longer than:
a. 24hrs
b. 2 hrs
c. 1hr
d. 48hrs - answer>>>b. 2 hrs
Residual moisture analysis shall not exceed % of initial weight by nuclear magnetic
resonance spectrometry:
a. 8%
b. 6%
c. 2%
d. 10% - answer>>>a. 8%
What is the storage conditions temp for commonly transplanted human tissue: cardiac
and vascular:
a. 1-10 degrees C
b. -100 degrees C
c. 10-10 degrees C
d. -20to 4-10 degrees C - answer>>>b. -100 degrees C
Processing, which includes dissection and packaging, of cardiac and vascular tissue
shall be preformed in a certified and qualified Class ( or cleaner) laminar flow:
a. 1000
b. 500
c. 100
d. 200 - answer>>>c. 100
To prevent additional warm ischemia and potential cellular or matrix damage caused by
temp cycling, methods shall be employed that maintain the tissue and solutions:
a. 1-40 degrees
C b.1-10
degrees C
c. -1- -5 degrees C
d. 5 -10 degrees C - answer>>>b.1-10 degrees C
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