PIL AB Questions and Answers for
Instant Practice
What sort of bedding is preferable during recovery from anaesthesia and why? -
Correct Answer ✔️✔️Dry-bed as sawdust and shaving can clog or abrade
the animal's eyes, nose, and mouth
What temperature should the recovery area be? - Correct Answer ✔️✔️30-
35 degrees until the animal has regained consciousness
Then 25-30 degrees for small rodents
What are the issues with using heating lamps? - Correct Answer ✔️✔️They
can cause damage to an animal if place too closely as they cannot move away
from the heat
What position should animals be placed in when recovering consciousness? -
Correct Answer ✔️✔️On their sides or on their chests
Would animals be given analgesia after a minor procedure? - Correct Answer
✔️✔️Minor procedures should only result in mild post-operative pain, but
,this should be assessed and controlled, for example by administration of an NSAID
such as Carprofen.
How should excess anaesthesia be disposed of? - Correct Answer ✔️✔
️Following the specific regulations set out by your animal unit (e.g. disposing of
small quantities of injectable anaesthetic remaining in a syringe in a hazardous
waste 'sharps' bin)
What are the immediate responses to handling? - Correct Answer ✔️✔
️Acute stress response
Increased heart rate and respiratory rate
Urination and defecation
Increased blood pressure
Increased blood glucose
What is the best technique for handling mice? - Correct Answer ✔️✔️Using
tunnels or cupping the mice in open hands
How can you avoid the use of oral dosing of compounds? - Correct Answer ✔
️✔️By training animals to ingest material voluntarily
,How can you avoid repeated blood sampling or intravenous injections? - Correct
Answer ✔️✔️Placement of an indwelling catheter
How can you avoid repeated injections of material? - Correct Answer ✔️✔
️Use of slow release pellets or osmotic minipumps
What should be done before carrying out a procedure on a live animal for the first
time? - Correct Answer ✔️✔️Observe and assist more experienced
colleagues
Practice the technique on a dead animal
What are the typical methods of administering substances to rodents? - Correct
Answer ✔️✔️Oral
Subcutaneous
Intramuscular
Introperitoneal
Intravenous
What are some of the risks of oral gavage? - Correct Answer ✔️✔
️Inadvertent dosing into the lung. This is recognised by the animal showing
, immediate signs of respiratory distress. The animal should then be immediately
humanely killed
What are the usual sites for subcutaneous injection in mice? - Correct Answer
✔️✔️Loose skin over the neck
Loose skin over the flank
Can you reuse the same needle to inject several animals? - Correct Answer ✔
️✔️No as the needle will become less sharp and cause more distress each
time
What is the best temperature for a fluid that is being injected? - Correct Answer
✔️✔️Body temperature to reduce any discomfort caused
What are some of the problems with intramuscular injection in mice? - Correct
Answer ✔️✔️Very small muscle mass makes it both technically difficult and
more painful due to distension of the muscle
Where can intramuscular injection in mice be done? - Correct Answer ✔️✔
️The front or back of the thigh
Instant Practice
What sort of bedding is preferable during recovery from anaesthesia and why? -
Correct Answer ✔️✔️Dry-bed as sawdust and shaving can clog or abrade
the animal's eyes, nose, and mouth
What temperature should the recovery area be? - Correct Answer ✔️✔️30-
35 degrees until the animal has regained consciousness
Then 25-30 degrees for small rodents
What are the issues with using heating lamps? - Correct Answer ✔️✔️They
can cause damage to an animal if place too closely as they cannot move away
from the heat
What position should animals be placed in when recovering consciousness? -
Correct Answer ✔️✔️On their sides or on their chests
Would animals be given analgesia after a minor procedure? - Correct Answer
✔️✔️Minor procedures should only result in mild post-operative pain, but
,this should be assessed and controlled, for example by administration of an NSAID
such as Carprofen.
How should excess anaesthesia be disposed of? - Correct Answer ✔️✔
️Following the specific regulations set out by your animal unit (e.g. disposing of
small quantities of injectable anaesthetic remaining in a syringe in a hazardous
waste 'sharps' bin)
What are the immediate responses to handling? - Correct Answer ✔️✔
️Acute stress response
Increased heart rate and respiratory rate
Urination and defecation
Increased blood pressure
Increased blood glucose
What is the best technique for handling mice? - Correct Answer ✔️✔️Using
tunnels or cupping the mice in open hands
How can you avoid the use of oral dosing of compounds? - Correct Answer ✔
️✔️By training animals to ingest material voluntarily
,How can you avoid repeated blood sampling or intravenous injections? - Correct
Answer ✔️✔️Placement of an indwelling catheter
How can you avoid repeated injections of material? - Correct Answer ✔️✔
️Use of slow release pellets or osmotic minipumps
What should be done before carrying out a procedure on a live animal for the first
time? - Correct Answer ✔️✔️Observe and assist more experienced
colleagues
Practice the technique on a dead animal
What are the typical methods of administering substances to rodents? - Correct
Answer ✔️✔️Oral
Subcutaneous
Intramuscular
Introperitoneal
Intravenous
What are some of the risks of oral gavage? - Correct Answer ✔️✔
️Inadvertent dosing into the lung. This is recognised by the animal showing
, immediate signs of respiratory distress. The animal should then be immediately
humanely killed
What are the usual sites for subcutaneous injection in mice? - Correct Answer
✔️✔️Loose skin over the neck
Loose skin over the flank
Can you reuse the same needle to inject several animals? - Correct Answer ✔
️✔️No as the needle will become less sharp and cause more distress each
time
What is the best temperature for a fluid that is being injected? - Correct Answer
✔️✔️Body temperature to reduce any discomfort caused
What are some of the problems with intramuscular injection in mice? - Correct
Answer ✔️✔️Very small muscle mass makes it both technically difficult and
more painful due to distension of the muscle
Where can intramuscular injection in mice be done? - Correct Answer ✔️✔
️The front or back of the thigh
