Molecular Biology Exam test queries
and answers graded A+
Nucleotide - ANS✅✅Monomer of DNA
Nucleoside - ANS✅✅Molecule of ribose plus base
2-deoxyribose - ANS✅✅sugar that has a H at the 2' C
Ribose - ANS✅✅Name of five carbon sugar that is found in RNA
Basepair - ANS✅✅Two nucleotides, one with a purine & one with a pyrimidine, connected by H
bonds
Antiparallel means - ANS✅✅the two polynucleotide chains/strands run in opposite directions
What two factors are the main determination of the Tm of DNA - ANS✅✅Amount of G:C's &
overall strand length
How many rings do purines & pyrimidines have? - ANS✅✅Purines: 2
Pyrimidines:1
The DNA from two different species can often be distinguished by a difference in the____. -
ANS✅✅Ratio of A+T to G+C
What is the difference between Uracil & Thymine - ANS✅✅Thymine has a methyl group
What did the X-ray diffraction patterns produced by Rosalind Franklin initially reveal about the DNA
molecule? - ANS✅✅It is a helical structure with distinct regularities
You have 340 nm of double stranded B form DNA. How many 360 degree turns are present in the
double helix? - ANS✅✅100
, You have 340 nm of double stranded B form DNA. How many nucleotides are present? Be careful! -
ANS✅✅2000
DNA and RNA both contain phosphate and deoxyribose. (True or False) - ANS✅✅False
In general, the keto form of thymine is more abundant than the enol form. (True or False) -
ANS✅✅True
Left-Handed helical form of DNA - ANS✅✅Z-DNA
DNA that results from reduced humidity around the DNA and is more tightly wound - ANS✅✅A-
DNA
DNA that most closely resembles the Watson & Crick model - ANS✅✅B-DNA
Term used to describe the amount of the double helix crossing over itself - ANS✅✅Writhe
Number of helical turns in a given space - ANS✅✅Twist
What are the main RNA secondary structures? - ANS✅✅Bulge-loop, hairpin, pseudoknot, internal
loop, and double helix
A closer association between DNA and histones most likely causes___. - ANS✅✅repression in gene
expression
Which amino acids of histone N-termini can be acetylated and deacetylated? - ANS✅✅Lysines
RNA can form Beta Sheets (True or False) - ANS✅✅False
What technique is most commonly used to study the secondary structure of RNA? -
ANS✅✅Computer algorithms
and answers graded A+
Nucleotide - ANS✅✅Monomer of DNA
Nucleoside - ANS✅✅Molecule of ribose plus base
2-deoxyribose - ANS✅✅sugar that has a H at the 2' C
Ribose - ANS✅✅Name of five carbon sugar that is found in RNA
Basepair - ANS✅✅Two nucleotides, one with a purine & one with a pyrimidine, connected by H
bonds
Antiparallel means - ANS✅✅the two polynucleotide chains/strands run in opposite directions
What two factors are the main determination of the Tm of DNA - ANS✅✅Amount of G:C's &
overall strand length
How many rings do purines & pyrimidines have? - ANS✅✅Purines: 2
Pyrimidines:1
The DNA from two different species can often be distinguished by a difference in the____. -
ANS✅✅Ratio of A+T to G+C
What is the difference between Uracil & Thymine - ANS✅✅Thymine has a methyl group
What did the X-ray diffraction patterns produced by Rosalind Franklin initially reveal about the DNA
molecule? - ANS✅✅It is a helical structure with distinct regularities
You have 340 nm of double stranded B form DNA. How many 360 degree turns are present in the
double helix? - ANS✅✅100
, You have 340 nm of double stranded B form DNA. How many nucleotides are present? Be careful! -
ANS✅✅2000
DNA and RNA both contain phosphate and deoxyribose. (True or False) - ANS✅✅False
In general, the keto form of thymine is more abundant than the enol form. (True or False) -
ANS✅✅True
Left-Handed helical form of DNA - ANS✅✅Z-DNA
DNA that results from reduced humidity around the DNA and is more tightly wound - ANS✅✅A-
DNA
DNA that most closely resembles the Watson & Crick model - ANS✅✅B-DNA
Term used to describe the amount of the double helix crossing over itself - ANS✅✅Writhe
Number of helical turns in a given space - ANS✅✅Twist
What are the main RNA secondary structures? - ANS✅✅Bulge-loop, hairpin, pseudoknot, internal
loop, and double helix
A closer association between DNA and histones most likely causes___. - ANS✅✅repression in gene
expression
Which amino acids of histone N-termini can be acetylated and deacetylated? - ANS✅✅Lysines
RNA can form Beta Sheets (True or False) - ANS✅✅False
What technique is most commonly used to study the secondary structure of RNA? -
ANS✅✅Computer algorithms
