MICRO 290 LAB UPDATED EXAM QUESTIONS AND
CORRECT ANSWERS WITH GUARANTEED PASS
Give examples of three general ways to eradicate microorganisms.
Physical (heat, UV light, etc.)
- biological (phage & antibiotic)
- chemical (bleach and alcohol)
Which microbial structure is most commonly harmed by UV light?
DNA
What kind of damage can UV do to bacteria's DNA, and what results from it?
DNA mutations caused by thymine dimers
Which UV light kind is the most deadly, and what is its wavelength?
UV-C; wavelength: 100–290 nm
show the UV light wavelength that the germicidal bulb emits. Provide examples
of the kinds of materials that are frequently sterilized (used to kill germs) using
these lights.
Sterilization of air, surfaces, water, and operating rooms is done at 254 nm.
After the microorganisms were frozen, what happened to them? After the
bacteria were agitated, heated, and dried, describe the results and the cause for
each species.
because bacterial endospores are extremely resilient to a variety of extreme
environments, such as heat, dryness, and UV light.
, Antibiotics are defined as a class of antimicrobial agent produced by
microorganisms that either inhibit or kill other microbes.
What is meant by antibiotic efficacy, and how is it quantified?
It indicates how well the antibiotics are destroying or suppressing the target
bacteria. The Kirby-Bauer method, which assesses the antibiotic sensitivity of
bacteria, is used to evaluate it.
What does the term "zone of inhibition" mean, and how does it relate to the
bacteria' susceptibility to the antibiotic under test?
A transparent circular region forms around the disk if antibiotics prevent the
bacterium from growing. The antibiotic is more successful at killing the
organism and is a better option for patients who are infected by that organism if
the zone of inhibition has a bigger diameter.
Which antibiotic has the largest and which the smallest zone of inhibition?
The biggest clear ring is C, while the smallest is B.
Which option would you pick first to treat your patient?
C (biggest transparent ring)
Which antibiotic would you use if C stands for penicillin and your patient has a
penicillin allergy?
D (the second-largest transparent ring)
What kind of antibiotic would you avoid?
B (the smallest transparent ring)
For the Kirby-Bauer test, what kind of agar is often used?
Hinton-Mueller Agar
What is epidemiology as a science?
the investigation of disease's causes, prevalence, and distribution within a
population
CORRECT ANSWERS WITH GUARANTEED PASS
Give examples of three general ways to eradicate microorganisms.
Physical (heat, UV light, etc.)
- biological (phage & antibiotic)
- chemical (bleach and alcohol)
Which microbial structure is most commonly harmed by UV light?
DNA
What kind of damage can UV do to bacteria's DNA, and what results from it?
DNA mutations caused by thymine dimers
Which UV light kind is the most deadly, and what is its wavelength?
UV-C; wavelength: 100–290 nm
show the UV light wavelength that the germicidal bulb emits. Provide examples
of the kinds of materials that are frequently sterilized (used to kill germs) using
these lights.
Sterilization of air, surfaces, water, and operating rooms is done at 254 nm.
After the microorganisms were frozen, what happened to them? After the
bacteria were agitated, heated, and dried, describe the results and the cause for
each species.
because bacterial endospores are extremely resilient to a variety of extreme
environments, such as heat, dryness, and UV light.
, Antibiotics are defined as a class of antimicrobial agent produced by
microorganisms that either inhibit or kill other microbes.
What is meant by antibiotic efficacy, and how is it quantified?
It indicates how well the antibiotics are destroying or suppressing the target
bacteria. The Kirby-Bauer method, which assesses the antibiotic sensitivity of
bacteria, is used to evaluate it.
What does the term "zone of inhibition" mean, and how does it relate to the
bacteria' susceptibility to the antibiotic under test?
A transparent circular region forms around the disk if antibiotics prevent the
bacterium from growing. The antibiotic is more successful at killing the
organism and is a better option for patients who are infected by that organism if
the zone of inhibition has a bigger diameter.
Which antibiotic has the largest and which the smallest zone of inhibition?
The biggest clear ring is C, while the smallest is B.
Which option would you pick first to treat your patient?
C (biggest transparent ring)
Which antibiotic would you use if C stands for penicillin and your patient has a
penicillin allergy?
D (the second-largest transparent ring)
What kind of antibiotic would you avoid?
B (the smallest transparent ring)
For the Kirby-Bauer test, what kind of agar is often used?
Hinton-Mueller Agar
What is epidemiology as a science?
the investigation of disease's causes, prevalence, and distribution within a
population
