Vesicular Transport 8/20
1. functions of vesicular transport: deliver newly made molecules to their final destination
communicate with extracellular environment
ingest extracellular particles and solutes
2. two major pathways of vesicular transport: secretory (ER to plasma membrane)
endocytic
3. secretory pathway definition: delivers newly made molecules to their proper positions within or
outside of the cell
4. endocytic pathway definition: delivers extracellular components to the inside of the cell
5. shematic of secretory pathway: ER
Vesicles
Golgi
vesicles
endosomes -> lysomes
plasma membrane
6. two concepts in the budding of vesicles: budding and fusion
1/4
, 7. protein coating membrane is needed for: helping to pinch the vesicle off of the donor mem-
brane
help to sort to the right plcace
8. three common proteins for protein coating membranes of vesicles: clathrin
COPI
COPII
9. clathrin structure: 3 heavy chains and 3 light chains which form triskelion
interation of triskelions formsa polyhedral lattice that surrounds vesicles
10. adaptin: binds clathrin and cargo receptors (an adaptor protein)
11. dynamin: G-protein required for release of clathrin-coated vesicles from membrane
12. formation and disassembly of clathrin coat: 1. adaptin - clathrin complex binds to cargo
receptor
2. bud starts to form
3. dynamin protein pinches off vesicle from membrane
4. clathrin and adaptin disassociate and are recycled and naked transport vesicle is released
2/4
1. functions of vesicular transport: deliver newly made molecules to their final destination
communicate with extracellular environment
ingest extracellular particles and solutes
2. two major pathways of vesicular transport: secretory (ER to plasma membrane)
endocytic
3. secretory pathway definition: delivers newly made molecules to their proper positions within or
outside of the cell
4. endocytic pathway definition: delivers extracellular components to the inside of the cell
5. shematic of secretory pathway: ER
Vesicles
Golgi
vesicles
endosomes -> lysomes
plasma membrane
6. two concepts in the budding of vesicles: budding and fusion
1/4
, 7. protein coating membrane is needed for: helping to pinch the vesicle off of the donor mem-
brane
help to sort to the right plcace
8. three common proteins for protein coating membranes of vesicles: clathrin
COPI
COPII
9. clathrin structure: 3 heavy chains and 3 light chains which form triskelion
interation of triskelions formsa polyhedral lattice that surrounds vesicles
10. adaptin: binds clathrin and cargo receptors (an adaptor protein)
11. dynamin: G-protein required for release of clathrin-coated vesicles from membrane
12. formation and disassembly of clathrin coat: 1. adaptin - clathrin complex binds to cargo
receptor
2. bud starts to form
3. dynamin protein pinches off vesicle from membrane
4. clathrin and adaptin disassociate and are recycled and naked transport vesicle is released
2/4
