Microbio 303 exam test quiz and
answers graded A+
Glucose Respiration in E. coli - ANS✅✅-Glucose is the electron donor
-oxidized to pyruvate
-electrons are transferred to NAD+
-pyruvate is further oxidized to carbon dioxide
- electrons are transferred to NAD+
- NADH transfers e- to ETC
-Electrons travel through ETC to generate PMF
- PMF is used to make ATP
- Electrons travel to electron acceptor
Glycolysis: The Emden Meyerhof Pathway - ANS✅✅-glucose is oxidized to 2 pyruvate in ten steps
-NAD+/NADH is the electron carrier
-2 ATP is made
Glucose Fermentation in E.Coli - ANS✅✅-uses pyruvate as electron acceptor
- creates byproducts such as lactate, succinate, ethanol, acetate, CO2, and H2
- Need to reoxidixe NADH
- ATP is made by SLP
Methanotrophs - ANS✅✅-Use Methane as Carbon source
-CO2 is electron acceptor
*This is respiration
-produces water
Methane monooxygenase - ANS✅✅-uses oxygen in the environment and methane to produce a
reduced form of methane which then goes on to create NADH for the cell.
Anaerobic Methane oxidation - ANS✅✅-uses nitrite as electron acceptor
, -Oxygen is the significant intermediate product consumed in the pathway
Anaerobic methane oxidizing consortium - ANS✅✅-methane oxidation is coupled with sulfate
reduction
Reaction Center: role and components - ANS✅✅-the reaction center is the site of electron
excitation
-contains multiple proteins and light-reactive pigments (chlorophylls)
Purple Sulfur Bacteria - ANS✅✅-PSII type photosystem
-Uses H2S as e- donor for photosystem
-photoautotrophs: fix CO2
Purple Nonsulfur Bacteria - ANS✅✅-PSII type photosystem
-can use low levels of H2S, other e- donors
-facultative photoautotrophs, can also grow as photoheterotroph or via aerobic respiration
-metabolically flexible: heterotrophs or autotrophs
-are often also able to grow with aerobic or anaerobic metabolism, or fermentation
Cyanobacteria - ANS✅✅-produce oxygen
-also known as blue-green algae
- originated oxygenic photosynthesis
- responsible for the oxygenation of Earth's atmosphere
Green Sulfur Bacteria - ANS✅✅-PSI type photosystem
-obligate anaerobes, use H2S as e- donor for photosystem
-photoautotrophs: Fix CO2
Green non-sulfur bacteria - ANS✅✅-Filamentous anoxygenic phototrophs
-PSII type photosystem
-Facultative photoautotrophs, can also grow as photoheterotroph or via aerobic respiration
*green bacteria use chlorosomes for enhanced light gathering
answers graded A+
Glucose Respiration in E. coli - ANS✅✅-Glucose is the electron donor
-oxidized to pyruvate
-electrons are transferred to NAD+
-pyruvate is further oxidized to carbon dioxide
- electrons are transferred to NAD+
- NADH transfers e- to ETC
-Electrons travel through ETC to generate PMF
- PMF is used to make ATP
- Electrons travel to electron acceptor
Glycolysis: The Emden Meyerhof Pathway - ANS✅✅-glucose is oxidized to 2 pyruvate in ten steps
-NAD+/NADH is the electron carrier
-2 ATP is made
Glucose Fermentation in E.Coli - ANS✅✅-uses pyruvate as electron acceptor
- creates byproducts such as lactate, succinate, ethanol, acetate, CO2, and H2
- Need to reoxidixe NADH
- ATP is made by SLP
Methanotrophs - ANS✅✅-Use Methane as Carbon source
-CO2 is electron acceptor
*This is respiration
-produces water
Methane monooxygenase - ANS✅✅-uses oxygen in the environment and methane to produce a
reduced form of methane which then goes on to create NADH for the cell.
Anaerobic Methane oxidation - ANS✅✅-uses nitrite as electron acceptor
, -Oxygen is the significant intermediate product consumed in the pathway
Anaerobic methane oxidizing consortium - ANS✅✅-methane oxidation is coupled with sulfate
reduction
Reaction Center: role and components - ANS✅✅-the reaction center is the site of electron
excitation
-contains multiple proteins and light-reactive pigments (chlorophylls)
Purple Sulfur Bacteria - ANS✅✅-PSII type photosystem
-Uses H2S as e- donor for photosystem
-photoautotrophs: fix CO2
Purple Nonsulfur Bacteria - ANS✅✅-PSII type photosystem
-can use low levels of H2S, other e- donors
-facultative photoautotrophs, can also grow as photoheterotroph or via aerobic respiration
-metabolically flexible: heterotrophs or autotrophs
-are often also able to grow with aerobic or anaerobic metabolism, or fermentation
Cyanobacteria - ANS✅✅-produce oxygen
-also known as blue-green algae
- originated oxygenic photosynthesis
- responsible for the oxygenation of Earth's atmosphere
Green Sulfur Bacteria - ANS✅✅-PSI type photosystem
-obligate anaerobes, use H2S as e- donor for photosystem
-photoautotrophs: Fix CO2
Green non-sulfur bacteria - ANS✅✅-Filamentous anoxygenic phototrophs
-PSII type photosystem
-Facultative photoautotrophs, can also grow as photoheterotroph or via aerobic respiration
*green bacteria use chlorosomes for enhanced light gathering
