ACS Instrumental Analysis Exam 2026
Questions and Answers
UV-Vis Source (Mono or Poly) - Correct answer-W-Halogen lamp; D2 or
H2Lamp; Xenon Lamp:(poly)
IR Source (Mono or Poly) - Correct answer-Nernst Glower; Globular;
Incandescent: (Poly)
Laser(Mono)
AA Source (mono or Poly) - Correct answer-HCl(mono)
AE Source (Mono or Poly) - Correct answer-Plasma Falme(poly)
Raman Source (mono or Poly) - Correct answer-He/Ne Laser;Ar Laser;Yag
Laser:(mono)
UV-Vis Sample Holder - Correct answer-Quartz, Glass, Plastic Cuvettes
IR Sample Holder - Correct answer-salt plat; gas chamber
AA Sample Holder - Correct answer-Graphite Tube; Flame
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,AE Sample Holder - Correct answer-inductively coupled plasma (ICP)
Raman Sample Holder - Correct answer-Cuvette
UV-Vis Wavelength Selector - Correct answer-Grating, Prism, Filter
IR Wavelength Selector - Correct answer-Interferometer; monochromator
AA Wavelength Selector - Correct answer-Monochrom. w/ grating
AE Wavelength Selector - Correct answer-Monochrom.
Raman Wavelength Selector - Correct answer-Monochrom. w/ grating; Infermom.
w/ laser
UV-Vis Detector - Correct answer-Photomultiplier Tube(PMT); Photodiode Array
IR Detector - Correct answer-Thermal(dispersion); pyroelectric; photoconducting
AA Detector - Correct answer-PMT
AE Detector - Correct answer-PMT; Photodiode Array
Raman Detector - Correct answer-PMT
HPLC Mobile Phase - Correct answer-Rev Phase: Polar Solvent
Norm. Phase: Nonpolar Solvent
GC Mobile Phase - Correct answer-He Gas
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,HPLC Column Length - Correct answer-10-30 cm
GC Column length - Correct answer-30-50 m
HPLC Modes of Operation - Correct answer-1. Isocratic
2. Gradient
GC Modes of Operation - Correct answer-1.Isothermal
2. Temp program
HPLC Injection System and Amount - Correct answer-Loop System (10uL)
GC Injection Syst. and Amount - Correct answer-Syringe (3-5uL)
HPLC Sationary Phase - Correct answer-NP=Polar
RP=Nonpolar
HPLC Detector - Correct answer-Z cell (UV)
GC Detector - Correct answer-FID (flame ionization detector)
HPLC Pump - Correct answer-Reciprocating
Displacement
HPLC Chromotography - Correct answer-1. Partition
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, 2. Adsorption
3. Ion Exchange
4. Size Exclusion
5. Affinity
what are the difference between calibration sensitivity and analytical sensitivity -
Correct answer-calibration sensitivity is the slope of a calibration curve at the
concentration of interest while analytical sensitivity is a response to the noise ratio
how to calculate calibration sensitivity - Correct answer-y=mx+b (m is the
calibration sensitivity)
graph concentration vs mean analytical signal
how to find the analytical sensitivity - Correct answer-As (concentation) =
calibration sensitivity/SD [ ]
how to find the coefficient of variation for the mean of each of the replicate sets -
Correct answer-CV = SD/Average x 100
how to find the detection limit for method? - Correct answer-
3.3*SD(blank)/calibration sensitivity
voltage in a series - Correct answer-Vt = Vc + Vb + Va
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Questions and Answers
UV-Vis Source (Mono or Poly) - Correct answer-W-Halogen lamp; D2 or
H2Lamp; Xenon Lamp:(poly)
IR Source (Mono or Poly) - Correct answer-Nernst Glower; Globular;
Incandescent: (Poly)
Laser(Mono)
AA Source (mono or Poly) - Correct answer-HCl(mono)
AE Source (Mono or Poly) - Correct answer-Plasma Falme(poly)
Raman Source (mono or Poly) - Correct answer-He/Ne Laser;Ar Laser;Yag
Laser:(mono)
UV-Vis Sample Holder - Correct answer-Quartz, Glass, Plastic Cuvettes
IR Sample Holder - Correct answer-salt plat; gas chamber
AA Sample Holder - Correct answer-Graphite Tube; Flame
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,AE Sample Holder - Correct answer-inductively coupled plasma (ICP)
Raman Sample Holder - Correct answer-Cuvette
UV-Vis Wavelength Selector - Correct answer-Grating, Prism, Filter
IR Wavelength Selector - Correct answer-Interferometer; monochromator
AA Wavelength Selector - Correct answer-Monochrom. w/ grating
AE Wavelength Selector - Correct answer-Monochrom.
Raman Wavelength Selector - Correct answer-Monochrom. w/ grating; Infermom.
w/ laser
UV-Vis Detector - Correct answer-Photomultiplier Tube(PMT); Photodiode Array
IR Detector - Correct answer-Thermal(dispersion); pyroelectric; photoconducting
AA Detector - Correct answer-PMT
AE Detector - Correct answer-PMT; Photodiode Array
Raman Detector - Correct answer-PMT
HPLC Mobile Phase - Correct answer-Rev Phase: Polar Solvent
Norm. Phase: Nonpolar Solvent
GC Mobile Phase - Correct answer-He Gas
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,HPLC Column Length - Correct answer-10-30 cm
GC Column length - Correct answer-30-50 m
HPLC Modes of Operation - Correct answer-1. Isocratic
2. Gradient
GC Modes of Operation - Correct answer-1.Isothermal
2. Temp program
HPLC Injection System and Amount - Correct answer-Loop System (10uL)
GC Injection Syst. and Amount - Correct answer-Syringe (3-5uL)
HPLC Sationary Phase - Correct answer-NP=Polar
RP=Nonpolar
HPLC Detector - Correct answer-Z cell (UV)
GC Detector - Correct answer-FID (flame ionization detector)
HPLC Pump - Correct answer-Reciprocating
Displacement
HPLC Chromotography - Correct answer-1. Partition
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, 2. Adsorption
3. Ion Exchange
4. Size Exclusion
5. Affinity
what are the difference between calibration sensitivity and analytical sensitivity -
Correct answer-calibration sensitivity is the slope of a calibration curve at the
concentration of interest while analytical sensitivity is a response to the noise ratio
how to calculate calibration sensitivity - Correct answer-y=mx+b (m is the
calibration sensitivity)
graph concentration vs mean analytical signal
how to find the analytical sensitivity - Correct answer-As (concentation) =
calibration sensitivity/SD [ ]
how to find the coefficient of variation for the mean of each of the replicate sets -
Correct answer-CV = SD/Average x 100
how to find the detection limit for method? - Correct answer-
3.3*SD(blank)/calibration sensitivity
voltage in a series - Correct answer-Vt = Vc + Vb + Va
©COPYRIGHT 2025, ALL RIGHTS RESERVED 4
