PORTAGE MICROBIOLOGY EXAM #5| Q & VERIFIED CORRECT
VERIFIED SOLUTION LATEST 2025 UPDATE
1. Define the concept of universal precautions (short answer) >> Universal
precautions means any and all samples, whether known or unknown, are to be treated as potentially hazardous
(or pathogenic) materials
2. List at lease 3 observations a researcher would be sure to note while assess-
ing and unknown microbial sample (short answer) >> Need to note >>
1. Size and shape
2. Any observable motility
3. Gram status (positive or negative)
3. While observing an unknown sample of limited amounts, a researcher must
determine the following observations >> 1) the presence of any motility and
2) its Gram status using the same sample- the liquid sample cannot be
divided. Which would you determine first and why? (short answer) >> You must
determine motility before determining Gram status. Motility requires a wet mount, while gram staining
requires heat fixing the sample. If one were to begin with the gram stain, the heat fixation process would kill the
organism, making any observations regarding motility impossible. The correct approach would be to place the liquid
culture on a glass slide and determine its motility status. Next, the same liquid culture can be heat fixed and gram
stained.
4. A facultative anaerobe is a microorganism capable of growth under what
conditions? (short answer) >> A facultative anaerobe is capable of growth under aerobic (with oxygen) and
anaerobic (without oxygen) conditions
5. True or False >> The Lancefield groups are used to subdivide antigenic
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, groups of alpha-hemolytic Streptococcus >> False. The Lancefield groups are used to subdivide
beta-hemolytic strep
6. The distinctions for Lancefield subgroups lie in its >> (select all that apply)
A. Catalase activity
B. Carbohydrate composition of antigens
C. Hemolytic activity
D. All of the above >> B. Carbohydrate composition of antigens
7. Rheumatic fever displays hemolytic activity and occurs when if left
untreated >> Beta >> strep. Pharyngitis
8. True or False >> Under most circumstances, staphylococcus can be found in
~80% of human population where it remains non-symptomatic >> False.
Staphylococcus in only found (non-symptomatic) in ~30% of the human population
2/
11
VERIFIED SOLUTION LATEST 2025 UPDATE
1. Define the concept of universal precautions (short answer) >> Universal
precautions means any and all samples, whether known or unknown, are to be treated as potentially hazardous
(or pathogenic) materials
2. List at lease 3 observations a researcher would be sure to note while assess-
ing and unknown microbial sample (short answer) >> Need to note >>
1. Size and shape
2. Any observable motility
3. Gram status (positive or negative)
3. While observing an unknown sample of limited amounts, a researcher must
determine the following observations >> 1) the presence of any motility and
2) its Gram status using the same sample- the liquid sample cannot be
divided. Which would you determine first and why? (short answer) >> You must
determine motility before determining Gram status. Motility requires a wet mount, while gram staining
requires heat fixing the sample. If one were to begin with the gram stain, the heat fixation process would kill the
organism, making any observations regarding motility impossible. The correct approach would be to place the liquid
culture on a glass slide and determine its motility status. Next, the same liquid culture can be heat fixed and gram
stained.
4. A facultative anaerobe is a microorganism capable of growth under what
conditions? (short answer) >> A facultative anaerobe is capable of growth under aerobic (with oxygen) and
anaerobic (without oxygen) conditions
5. True or False >> The Lancefield groups are used to subdivide antigenic
1/
11
, groups of alpha-hemolytic Streptococcus >> False. The Lancefield groups are used to subdivide
beta-hemolytic strep
6. The distinctions for Lancefield subgroups lie in its >> (select all that apply)
A. Catalase activity
B. Carbohydrate composition of antigens
C. Hemolytic activity
D. All of the above >> B. Carbohydrate composition of antigens
7. Rheumatic fever displays hemolytic activity and occurs when if left
untreated >> Beta >> strep. Pharyngitis
8. True or False >> Under most circumstances, staphylococcus can be found in
~80% of human population where it remains non-symptomatic >> False.
Staphylococcus in only found (non-symptomatic) in ~30% of the human population
2/
11
