1
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MB (ASCP) Questions and Answers (100%
Correct Answers) Already Graded A+
Consider the table below where a child and a possible father (PF)
share the listed paternity indices for each locus listed (LOC-A1,
LOC-B2, LOC-C3, LOC-D4).
Locus Tested PF Child Paternity Index
© 2025 Assignment Expert
LOC-A1 3 2/3 2.18
LOC-B2 7/5 5 0.798
LOC-C3 15/17 9/17 5.21
Guru01 - Stuvia
LOC-D4 12 12 1.37
Based on the data presented in the table, what is the combined
paternity index, CPI, from the loci tested: LOC-A1, LOC-B2, LOC-
C3 and LOC-D4?
12.42
9.558
15.56
2.38 [ ANS: ] 12.42 ( CPI is calculated by multiplying all Paternity
index tgt)
Best way to avoid PCR contamination [ ANS: ] Separate Pre-PCR
(clean area) and post PCR (dirty area)
A260=1.4, A280=1.1 . protein contamination or Rna contam or
useable RNA [ ANS: ] Protein contamination . A260/280 = 1.3
, 2
For Expert help and assignment solutions, +254707240657
<1.6 = protein contamination, 1.7-2.0 = good DNA, 1.9-2.0 = good
RNA
Why is Fluorometry a better technique that Spectroscopy [ ANS: ]
Increased sensitivity and more importantly can accurately
measure DNA concentration of a structurally intact double
stranded DNA molecule
Linkage Disequilibrium [ ANS: ] LD is the non random association of
alleles at 2 or more loci in a general population. When alleles are
in LD, haplotypes do not occur at the expected frequencies.
Specificity [ ANS: ] Specificity = TN / (TN + FP)
© 2025 Assignment Expert
Limit of Detection (LOD) [ ANS: ] lowest concentration of analyte
that can be detected
Guru01 - Stuvia
mRNA Sequence provided - Convert it into DNA [ ANS: ]
(Template/Anti-sense Strand) [Replace U with T]
Correct order of HIV Sample Preparation Workflow [ ANS: ] Plasma
Preparation
RNA Extraction
RNA Amplification/Modification Procedure
If a flask of cells is provided for the intended purpose of DNA
isolation, how would you proceed further [ ANS: ] Trypsinize and/or
scrape the cells inside the provided flask, collect the cells
suspended in the media, spin, and then proceed further
Which end of the primer is critical for the increased specification
of PCR Amplification process [ ANS: ] 3'End of the Primer
Primer Dimer Complementarity [ ANS: ] 5' End of the Primers (3-3'
Was not an option)
Mechanism of Action of MecA Gene in MRSA [ ANS: ] Altered Drug
Target (PBP2A)
, 3
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Technique to be used for FFPE Cervical Tissue Provided [ ANS: ]
Invader Assay (Non-Amplification Method)
Liquid Based-Cytology Useful for what kind of virus [ ANS: ] HPV is
the correct answer (Options Provided for: HIV, HBV, HCV, HPV)
Isothermal Method that utilizes Primes and Polymerase to Create
Synthetic dsDNA [ ANS: ] SDA (Strand Displacement Amplification)
Polymerase used in Isothermal LAMP Procedure [ ANS: ] BST
(Bacillus Stearothermophilus)
BRAF V600E Mutation is Associated with [ ANS: ] Lynch Syndrome
© 2025 Assignment Expert
(Also Known as HNPCC)
CAP Requires Discontinued SOP/Procedures to be stored for how
long [ ANS: ] At least 2 years from the date of discontinuation
Guru01 - Stuvia
CAP Requires Laboratory Personnel (Technologist) Proficiency
Testing [ ANS: ] Once a Year
Most Important Component of Clinical Laboratory Completed
Validation Studies [ ANS: ] Final Test Validation Summary Signed off
by Laboratory Director
STR Electropherogram provided and select the most informative
loci [ ANS: ] Two different alleles each was both recipient vs donor
the most informative one
STR makes use of which of the following [ ANS: ] Variable
Nucleotide
Experiment Performed (RFLP + PCR + Gel Electrophoresis): three
bands observed in the mutant sequence as compared to the
normal sequence [ ANS: ] Insertion of New Restriction Site
Mutation in what gene decreases metabolism of Irinotecan Drug [
ANS: ] UGT1A1
CYP2D6 Enzyme Metabolizes what drug [ ANS: ] Codeine
, 4
For Expert help and assignment solutions, +254707240657
Determine Melting Temperature of a given DNA Sequence [ ANS: ]
2(A+T) + 4(G+C)
Specimen Collection for Testing of Cystic Fibrosis [ ANS: ] Either 1 ml
EDTA Lavender Tube/5 ml ACD Tube (Not Sure: I think I answered 1
ml EDTA, however the volume seems to be too low)
Fish is used in [ ANS: ] Prader Willi Syndrome
Defects associated with Prader Willi Syndrome [ ANS: ] Uniparental
Disomy and Genomic Imprinting - Paternal Chromsome Deletion
15
© 2025 Assignment Expert
Which of the Following is Trinucleotide Repeat Expansion Disorder [
ANS: ] Huntington's Disease, Fragile X
HER2/EGFR2 Overexpression [ ANS: ] Trastuzumab Therapy
Guru01 - Stuvia
Overexpression of estrogen, so treat with Herceptin/Tamoxifin
Trastuzumab therapy
T-Cell Receptor Gene Rearrangement [ ANS: ] Hodgkin
Lymphoma (PS: Don't get confused with T-Cell Lymphoma that
corresponds to Sezary Syndrome, both options will be present for
confusion)
Genes Involved in the Cell Cycle Process are termed as [ ANS: ]
Cycle-Dependent
What is TP53 [ ANS: ] Tumor Suppressor Gene
PML/RARA Fusion occurs in which Leukemia [ ANS: ] AP (Bit Strange
2 questions on the same leukemia)
Acute Promyleocytic Lekemia (APL) Translocation Corresponds to
[ ANS: ] t(15;17)
BAM/SAM Files Purpose [ ANS: ] Contains the Sequenced Aligned
Data
For Expert help and assignment solutions, +254707240657
MB (ASCP) Questions and Answers (100%
Correct Answers) Already Graded A+
Consider the table below where a child and a possible father (PF)
share the listed paternity indices for each locus listed (LOC-A1,
LOC-B2, LOC-C3, LOC-D4).
Locus Tested PF Child Paternity Index
© 2025 Assignment Expert
LOC-A1 3 2/3 2.18
LOC-B2 7/5 5 0.798
LOC-C3 15/17 9/17 5.21
Guru01 - Stuvia
LOC-D4 12 12 1.37
Based on the data presented in the table, what is the combined
paternity index, CPI, from the loci tested: LOC-A1, LOC-B2, LOC-
C3 and LOC-D4?
12.42
9.558
15.56
2.38 [ ANS: ] 12.42 ( CPI is calculated by multiplying all Paternity
index tgt)
Best way to avoid PCR contamination [ ANS: ] Separate Pre-PCR
(clean area) and post PCR (dirty area)
A260=1.4, A280=1.1 . protein contamination or Rna contam or
useable RNA [ ANS: ] Protein contamination . A260/280 = 1.3
, 2
For Expert help and assignment solutions, +254707240657
<1.6 = protein contamination, 1.7-2.0 = good DNA, 1.9-2.0 = good
RNA
Why is Fluorometry a better technique that Spectroscopy [ ANS: ]
Increased sensitivity and more importantly can accurately
measure DNA concentration of a structurally intact double
stranded DNA molecule
Linkage Disequilibrium [ ANS: ] LD is the non random association of
alleles at 2 or more loci in a general population. When alleles are
in LD, haplotypes do not occur at the expected frequencies.
Specificity [ ANS: ] Specificity = TN / (TN + FP)
© 2025 Assignment Expert
Limit of Detection (LOD) [ ANS: ] lowest concentration of analyte
that can be detected
Guru01 - Stuvia
mRNA Sequence provided - Convert it into DNA [ ANS: ]
(Template/Anti-sense Strand) [Replace U with T]
Correct order of HIV Sample Preparation Workflow [ ANS: ] Plasma
Preparation
RNA Extraction
RNA Amplification/Modification Procedure
If a flask of cells is provided for the intended purpose of DNA
isolation, how would you proceed further [ ANS: ] Trypsinize and/or
scrape the cells inside the provided flask, collect the cells
suspended in the media, spin, and then proceed further
Which end of the primer is critical for the increased specification
of PCR Amplification process [ ANS: ] 3'End of the Primer
Primer Dimer Complementarity [ ANS: ] 5' End of the Primers (3-3'
Was not an option)
Mechanism of Action of MecA Gene in MRSA [ ANS: ] Altered Drug
Target (PBP2A)
, 3
For Expert help and assignment solutions, +254707240657
Technique to be used for FFPE Cervical Tissue Provided [ ANS: ]
Invader Assay (Non-Amplification Method)
Liquid Based-Cytology Useful for what kind of virus [ ANS: ] HPV is
the correct answer (Options Provided for: HIV, HBV, HCV, HPV)
Isothermal Method that utilizes Primes and Polymerase to Create
Synthetic dsDNA [ ANS: ] SDA (Strand Displacement Amplification)
Polymerase used in Isothermal LAMP Procedure [ ANS: ] BST
(Bacillus Stearothermophilus)
BRAF V600E Mutation is Associated with [ ANS: ] Lynch Syndrome
© 2025 Assignment Expert
(Also Known as HNPCC)
CAP Requires Discontinued SOP/Procedures to be stored for how
long [ ANS: ] At least 2 years from the date of discontinuation
Guru01 - Stuvia
CAP Requires Laboratory Personnel (Technologist) Proficiency
Testing [ ANS: ] Once a Year
Most Important Component of Clinical Laboratory Completed
Validation Studies [ ANS: ] Final Test Validation Summary Signed off
by Laboratory Director
STR Electropherogram provided and select the most informative
loci [ ANS: ] Two different alleles each was both recipient vs donor
the most informative one
STR makes use of which of the following [ ANS: ] Variable
Nucleotide
Experiment Performed (RFLP + PCR + Gel Electrophoresis): three
bands observed in the mutant sequence as compared to the
normal sequence [ ANS: ] Insertion of New Restriction Site
Mutation in what gene decreases metabolism of Irinotecan Drug [
ANS: ] UGT1A1
CYP2D6 Enzyme Metabolizes what drug [ ANS: ] Codeine
, 4
For Expert help and assignment solutions, +254707240657
Determine Melting Temperature of a given DNA Sequence [ ANS: ]
2(A+T) + 4(G+C)
Specimen Collection for Testing of Cystic Fibrosis [ ANS: ] Either 1 ml
EDTA Lavender Tube/5 ml ACD Tube (Not Sure: I think I answered 1
ml EDTA, however the volume seems to be too low)
Fish is used in [ ANS: ] Prader Willi Syndrome
Defects associated with Prader Willi Syndrome [ ANS: ] Uniparental
Disomy and Genomic Imprinting - Paternal Chromsome Deletion
15
© 2025 Assignment Expert
Which of the Following is Trinucleotide Repeat Expansion Disorder [
ANS: ] Huntington's Disease, Fragile X
HER2/EGFR2 Overexpression [ ANS: ] Trastuzumab Therapy
Guru01 - Stuvia
Overexpression of estrogen, so treat with Herceptin/Tamoxifin
Trastuzumab therapy
T-Cell Receptor Gene Rearrangement [ ANS: ] Hodgkin
Lymphoma (PS: Don't get confused with T-Cell Lymphoma that
corresponds to Sezary Syndrome, both options will be present for
confusion)
Genes Involved in the Cell Cycle Process are termed as [ ANS: ]
Cycle-Dependent
What is TP53 [ ANS: ] Tumor Suppressor Gene
PML/RARA Fusion occurs in which Leukemia [ ANS: ] AP (Bit Strange
2 questions on the same leukemia)
Acute Promyleocytic Lekemia (APL) Translocation Corresponds to
[ ANS: ] t(15;17)
BAM/SAM Files Purpose [ ANS: ] Contains the Sequenced Aligned
Data
