ASCP CG Exam (Classic Cytogenetics)
When should samples be logged in? - CORRECT ANSWER-As soon as they arrive in
the laboratory.
How long do you wait before requesting an amniotic fluid if no cell attachment is
observed? - CORRECT ANSWER-10 days.
All of the following will result in culture failure except: - CORRECT ANSWER-Transport
at room temp.
Which cells would you want to use for long term serial cytogenetic studies? - CORRECT
ANSWER-Fibroblasts.
The following enzymes can be used to dissociate chorionic villus samples except for: -
CORRECT ANSWER-RNAse.
Which of the following is not a necessary ingredient which is added to modified
medium? - CORRECT ANSWER-Antibiotics.
All of the following have buffering capability except: - CORRECT ANSWER-L-
Glutamine.
What type of media is necessary for the culture of Fragile X cases? - CORRECT
ANSWER-Folic acid deficient media.
What are the basics of in situ hybridization? - CORRECT ANSWER-Pretreatment of
slides with acid, extraction of DNA with barium hydroxide, and incubation in 2xSSC to
promote denaturation.
The following are indicators for amniocentesis except: - CORRECT ANSWER-A family
history of an autosomal recessive disorder.
When a bone marrow sample cannot be delivered to the lab within 4 hrs, the sample
should: - CORRECT ANSWER-Be put in media and kept at room temp.
A PB sample is clotted. What do you do? - CORRECT ANSWER-Break up the clot.
All of the following information is required during the specimen set up procedure except:
- CORRECT ANSWER-Infectious agent.
,The most important reason why Biological Safety Caninets are used instead of
Horizontal Laminar Flow Hoods in the handling of human tissues is: - CORRECT
ANSWER-To protect the technologist who is processing the specimen.
In which of the following situations would one suspect bacterial contamination? -
CORRECT ANSWER-The culture media is turbid in appearance.
Which of the following can be used for serial culture from amniotic fluid? - CORRECT
ANSWER-Fibroblasts.
Which of the following chemicals is used for cryopreservation? - CORRECT ANSWER-
Dimethyl sulfoxide (DMSO).
Adding the drug Colcemid to cell cultures can cause all of the following phenomenons to
occur except: - CORRECT ANSWER-Cell swelling.
During slidemaking all of the following factors are important except for: - CORRECT
ANSWER-Slides are 1st being dipped in acetic acid.
A good banding pattern will be expected when we observe chromosomes before
banding under the phase scope with the following appearance: - CORRECT ANSWER-
Dark.
Which of the following factors are not important for chromosome spreading? -
CORRECT ANSWER-Mitotic index.
The best method to process slides for G-banding on the same day that they are
dropped is: - CORRECT ANSWER-Incubate the slide in 90°C for 1-3 hrs before
banding.
You are analyzing a clinical case and discover a small extra marker with satellites. What
staining technique would be most useful in determining the chromosomal origin of this
marker? - CORRECT ANSWER-DAPI/DA.
When using a fluorescein labeled probe during the FISH procedure, the appropriate
counter stain used should be: - CORRECT ANSWER-Propidium iodide.
When doing sequential staining which of the following is used to remove oil from slides?
- CORRECT ANSWER-Xylene.
What is the best banding technique to distinguish between 47,XY,+18 and 47,XYY? -
CORRECT ANSWER-QFQ.
When the identification of paired chromosomes is the primary goal, which if the
following banding techniques should be used? - CORRECT ANSWER-G-banding.
, When the addition of non homologous material to the q-arm of chromosome 16 is
suspected after G-banding, which if the following banding techniques should be used? -
CORRECT ANSWER-C-banding.
Which chromosome is most different between C- and G-banding? - CORRECT
ANSWER-9.
What is not a criteria for G-band identification? - CORRECT ANSWER-Location of the
telomere.
What do quinacrine and acridine orange dyes stain? - CORRECT ANSWER-AT rich
DNA.
What type of container should be used for collecting peripheral blood and bone marrow
specimens? - CORRECT ANSWER-Green top tube coated with sodium heparin.
What type of container should be used for collecting solid tissue samples? - CORRECT
ANSWER-A sterile container containing media, supplemented with antibiotics.
How do you avoid microbial contamination? - CORRECT ANSWER-By using aseptic
technique, sterile containers and antibiotics/fungicides.
How do you avoid cellular contamination of prenatal specimens? - CORRECT
ANSWER-Discard the 1st 1-2 cc of amniotic fluid drawn.
How do you avoid cellular contamination of solid tumor specimens? - CORRECT
ANSWER-Dissect away normal parenchyma and fatty tissue.
How much specimen is required for the successful analysis of a peripheral blood
specimen? - CORRECT ANSWER-5-10 ml for adults and 3-5 ml for children.
How much specimen is required for the successful analysis of a bone marrow
specimen? - CORRECT ANSWER-1-3 ml.
How much specimen is required for the successful analysis of a prenatal specimen? -
CORRECT ANSWER-15-30 ml of amniotic fluid collected in two separate containers.
How much specimen is required for the successful analysis of solid tissues? -
CORRECT ANSWER-5-25 mg of CVS, 1 mm^3 of skin biopsy and 1cm^3 of solid
tumor.
What should the label of each collection container always have? - CORRECT
ANSWER-Patient name and identification number.
What does penicillin/streptomycin treat? - CORRECT ANSWER-Bacterial
contamination.
When should samples be logged in? - CORRECT ANSWER-As soon as they arrive in
the laboratory.
How long do you wait before requesting an amniotic fluid if no cell attachment is
observed? - CORRECT ANSWER-10 days.
All of the following will result in culture failure except: - CORRECT ANSWER-Transport
at room temp.
Which cells would you want to use for long term serial cytogenetic studies? - CORRECT
ANSWER-Fibroblasts.
The following enzymes can be used to dissociate chorionic villus samples except for: -
CORRECT ANSWER-RNAse.
Which of the following is not a necessary ingredient which is added to modified
medium? - CORRECT ANSWER-Antibiotics.
All of the following have buffering capability except: - CORRECT ANSWER-L-
Glutamine.
What type of media is necessary for the culture of Fragile X cases? - CORRECT
ANSWER-Folic acid deficient media.
What are the basics of in situ hybridization? - CORRECT ANSWER-Pretreatment of
slides with acid, extraction of DNA with barium hydroxide, and incubation in 2xSSC to
promote denaturation.
The following are indicators for amniocentesis except: - CORRECT ANSWER-A family
history of an autosomal recessive disorder.
When a bone marrow sample cannot be delivered to the lab within 4 hrs, the sample
should: - CORRECT ANSWER-Be put in media and kept at room temp.
A PB sample is clotted. What do you do? - CORRECT ANSWER-Break up the clot.
All of the following information is required during the specimen set up procedure except:
- CORRECT ANSWER-Infectious agent.
,The most important reason why Biological Safety Caninets are used instead of
Horizontal Laminar Flow Hoods in the handling of human tissues is: - CORRECT
ANSWER-To protect the technologist who is processing the specimen.
In which of the following situations would one suspect bacterial contamination? -
CORRECT ANSWER-The culture media is turbid in appearance.
Which of the following can be used for serial culture from amniotic fluid? - CORRECT
ANSWER-Fibroblasts.
Which of the following chemicals is used for cryopreservation? - CORRECT ANSWER-
Dimethyl sulfoxide (DMSO).
Adding the drug Colcemid to cell cultures can cause all of the following phenomenons to
occur except: - CORRECT ANSWER-Cell swelling.
During slidemaking all of the following factors are important except for: - CORRECT
ANSWER-Slides are 1st being dipped in acetic acid.
A good banding pattern will be expected when we observe chromosomes before
banding under the phase scope with the following appearance: - CORRECT ANSWER-
Dark.
Which of the following factors are not important for chromosome spreading? -
CORRECT ANSWER-Mitotic index.
The best method to process slides for G-banding on the same day that they are
dropped is: - CORRECT ANSWER-Incubate the slide in 90°C for 1-3 hrs before
banding.
You are analyzing a clinical case and discover a small extra marker with satellites. What
staining technique would be most useful in determining the chromosomal origin of this
marker? - CORRECT ANSWER-DAPI/DA.
When using a fluorescein labeled probe during the FISH procedure, the appropriate
counter stain used should be: - CORRECT ANSWER-Propidium iodide.
When doing sequential staining which of the following is used to remove oil from slides?
- CORRECT ANSWER-Xylene.
What is the best banding technique to distinguish between 47,XY,+18 and 47,XYY? -
CORRECT ANSWER-QFQ.
When the identification of paired chromosomes is the primary goal, which if the
following banding techniques should be used? - CORRECT ANSWER-G-banding.
, When the addition of non homologous material to the q-arm of chromosome 16 is
suspected after G-banding, which if the following banding techniques should be used? -
CORRECT ANSWER-C-banding.
Which chromosome is most different between C- and G-banding? - CORRECT
ANSWER-9.
What is not a criteria for G-band identification? - CORRECT ANSWER-Location of the
telomere.
What do quinacrine and acridine orange dyes stain? - CORRECT ANSWER-AT rich
DNA.
What type of container should be used for collecting peripheral blood and bone marrow
specimens? - CORRECT ANSWER-Green top tube coated with sodium heparin.
What type of container should be used for collecting solid tissue samples? - CORRECT
ANSWER-A sterile container containing media, supplemented with antibiotics.
How do you avoid microbial contamination? - CORRECT ANSWER-By using aseptic
technique, sterile containers and antibiotics/fungicides.
How do you avoid cellular contamination of prenatal specimens? - CORRECT
ANSWER-Discard the 1st 1-2 cc of amniotic fluid drawn.
How do you avoid cellular contamination of solid tumor specimens? - CORRECT
ANSWER-Dissect away normal parenchyma and fatty tissue.
How much specimen is required for the successful analysis of a peripheral blood
specimen? - CORRECT ANSWER-5-10 ml for adults and 3-5 ml for children.
How much specimen is required for the successful analysis of a bone marrow
specimen? - CORRECT ANSWER-1-3 ml.
How much specimen is required for the successful analysis of a prenatal specimen? -
CORRECT ANSWER-15-30 ml of amniotic fluid collected in two separate containers.
How much specimen is required for the successful analysis of solid tissues? -
CORRECT ANSWER-5-25 mg of CVS, 1 mm^3 of skin biopsy and 1cm^3 of solid
tumor.
What should the label of each collection container always have? - CORRECT
ANSWER-Patient name and identification number.
What does penicillin/streptomycin treat? - CORRECT ANSWER-Bacterial
contamination.
