OXIDATIVE PHOSPHORYLATION CHAPTER 14 TEST
QUESTIONS AND CORRECT ANSWERS
Which phases of the electron-transport chain are carried by Coenzyme Q?
Verify anything that applies. ANSWER Complexes I and III Complex II and III
Analyze the yeast ATP synthase partial structure molecular model. What
portion of the molecule crosses the inner membrane of the mitochondria?
ANSWER F0 rotor
The proton gradient drives the F0 rotor's rotation. Examine the E. Coli F0 rotor.
The protons are transferred to a(n) __________ after entering via the input half-
channel. ANSWER unprotonated Asp residue on the C-terminal helix of one "c"
subunit
The rotation of the c-ring in the E. coli F0 rotor molecular model is directly
caused by __________. ANSWER When Asp61 deprotonates, the C-terminal
helix in a "c" subunit twists.
Analyze the ATP synthase's F1 complex from the mitochondria of the cow's
heart. What stops the F0 c-ring complex from rotating with this F1 complex?
ANSWER The stator, which is attached to the stationary "a" component of F0,
binds it.
As shown in the tutorial's part II on the molecular model of the E. coli enzyme,
consider an ATP synthase complex with 12 "c" subunits in its F0 rotor. To
produce ten ATP molecules, how many protons need to move through the F0
complex? ANSWER 40
Dinitrophenol and other uncouplers were considered as weight-control drugs
years ago. It is conceivable that fat might be burned without ATP synthesis
occurring simultaneously, allowing for the re-formation of fat or carbohydrates.
, Why was this a terrible idea—that is, a deadly one?
Fill in the blanks in the sentence on the right with the objects in the left column.
ANSWER When dinitrophenol is present, ATP production is inhibited, causing
energy to be released as heat and the victims to have uncontrollable fevers.
At first glance, the sheer volume of intermediate metabolism's reactions,
intermediates, and routes may seem daunting. All of these, nevertheless, can be
made simpler by thinking of metabolism as a road map. This makes it possible
to concentrate on the strategy as a whole or on a specific area of the map. Fill in
the blanks to provide a summary of metabolism in this activity.
In the sentences on the right, match the words in the left column to the
corresponding blanks. ANSWER Catabolism and anabolism are the two
subcategories of metabolism. Complex organic compounds are broken down
into simpler ones by catabolism, which frequently results in the release of
energy. Anabolism, which frequently involves energy consumption, creates
macromolecules from simpler chemicals.
Acetyl-CoA and other simple two-carbon chemical compounds are used as
frequent intermediates in both routes. Following their entry into the citric acid
cycle, these intermediates undergo further oxidation to produce carbon dioxide,
reduced electron carriers, and trace amounts of ATP. In order to produce a
proton (H+) gradient, the electrons on these carriers are ultimately transferred
via the electron transport chain. ATP is produced when this proton gradient is
released.
There are three distinct phases to the citric acid cycle. The first step transforms
metabolic resources (fats, carbohydrates, and amino acids) into a common
currency that the second stage uses. The citric acid cycle proper is the second
step, which is a substrate cycle in the mitochondrial matrix. The primary source
of cellular energy is the third step, which takes place in the inner mitochondrial
membrane. Assign the following characteristics to the appropriate level.
Stage 1 involves processes that take place prior to the citric acid cycle, Stage 2
involves the citric acid cycle, and Stage 3 involves processes that take place
following the citric acid cycle.
Drag the relevant objects into the corresponding containers. ANSWER Stage 1:
Pyruvate conversion to acetyl-CoA
QUESTIONS AND CORRECT ANSWERS
Which phases of the electron-transport chain are carried by Coenzyme Q?
Verify anything that applies. ANSWER Complexes I and III Complex II and III
Analyze the yeast ATP synthase partial structure molecular model. What
portion of the molecule crosses the inner membrane of the mitochondria?
ANSWER F0 rotor
The proton gradient drives the F0 rotor's rotation. Examine the E. Coli F0 rotor.
The protons are transferred to a(n) __________ after entering via the input half-
channel. ANSWER unprotonated Asp residue on the C-terminal helix of one "c"
subunit
The rotation of the c-ring in the E. coli F0 rotor molecular model is directly
caused by __________. ANSWER When Asp61 deprotonates, the C-terminal
helix in a "c" subunit twists.
Analyze the ATP synthase's F1 complex from the mitochondria of the cow's
heart. What stops the F0 c-ring complex from rotating with this F1 complex?
ANSWER The stator, which is attached to the stationary "a" component of F0,
binds it.
As shown in the tutorial's part II on the molecular model of the E. coli enzyme,
consider an ATP synthase complex with 12 "c" subunits in its F0 rotor. To
produce ten ATP molecules, how many protons need to move through the F0
complex? ANSWER 40
Dinitrophenol and other uncouplers were considered as weight-control drugs
years ago. It is conceivable that fat might be burned without ATP synthesis
occurring simultaneously, allowing for the re-formation of fat or carbohydrates.
, Why was this a terrible idea—that is, a deadly one?
Fill in the blanks in the sentence on the right with the objects in the left column.
ANSWER When dinitrophenol is present, ATP production is inhibited, causing
energy to be released as heat and the victims to have uncontrollable fevers.
At first glance, the sheer volume of intermediate metabolism's reactions,
intermediates, and routes may seem daunting. All of these, nevertheless, can be
made simpler by thinking of metabolism as a road map. This makes it possible
to concentrate on the strategy as a whole or on a specific area of the map. Fill in
the blanks to provide a summary of metabolism in this activity.
In the sentences on the right, match the words in the left column to the
corresponding blanks. ANSWER Catabolism and anabolism are the two
subcategories of metabolism. Complex organic compounds are broken down
into simpler ones by catabolism, which frequently results in the release of
energy. Anabolism, which frequently involves energy consumption, creates
macromolecules from simpler chemicals.
Acetyl-CoA and other simple two-carbon chemical compounds are used as
frequent intermediates in both routes. Following their entry into the citric acid
cycle, these intermediates undergo further oxidation to produce carbon dioxide,
reduced electron carriers, and trace amounts of ATP. In order to produce a
proton (H+) gradient, the electrons on these carriers are ultimately transferred
via the electron transport chain. ATP is produced when this proton gradient is
released.
There are three distinct phases to the citric acid cycle. The first step transforms
metabolic resources (fats, carbohydrates, and amino acids) into a common
currency that the second stage uses. The citric acid cycle proper is the second
step, which is a substrate cycle in the mitochondrial matrix. The primary source
of cellular energy is the third step, which takes place in the inner mitochondrial
membrane. Assign the following characteristics to the appropriate level.
Stage 1 involves processes that take place prior to the citric acid cycle, Stage 2
involves the citric acid cycle, and Stage 3 involves processes that take place
following the citric acid cycle.
Drag the relevant objects into the corresponding containers. ANSWER Stage 1:
Pyruvate conversion to acetyl-CoA
