Pipetting: master the technique || 100% VERIFIED!!!
The Bradford assay only works at low protein concentrations from .05 2.5 mg/mL proteins.
How can I reduce the concentration of protein in our sample?
A.Evaporate the sample
B.Dilute the sample
C. Concentrate the sample
D. Purify the sample correct answers B. Dilute the sample
We will make a dilution series of our protein, to find a concentration that falls into the range
of measurable concentrations
Why are only sterile Pipette tips in the lab?
A. To prevent reproduction of proteins
B. To avoid contamination of the sample
C. To make sure that the tips can be reused
D. To make pipetting more precise correct answers B. To avoid contamination of the sample
(To sterilize pipette tips, they are heated to 120 Celsius under high pressure which kills most
germs
what is the upper limit of a p1000 pipette correct answers 1000 microliters
what is the lower limit of a pipette p1000 correct answers 200 micro-liters
What is the volume range of the P1000
a. 20-200 ul
b. 2-20 ul
c. .5-2 ul
d. 200-1000ul correct answers d.. 200-1000ul
What are the ranges for the 3 pipettes? correct answers P1000: 200-1000ul
P200: 20-200ul
P20: 2-20ul
How would you set the volume to pipette 20 ul with the P20
a. 200
b. 2
c. 20
d. 2000 correct answers a. 200
why did you press the plunger all the way down to the second stop?
a. to mix the contents in the tube
b. to push out the remaining fluid from the tip
c, to avoid contaminations
d. to draw up liquid correct answers b. to make sure that every drop of liquid is transferred to
the micro centrifuge to if we press the plunger to the second stop
What do you need to do next before pipetting a different sample?
a. discard the pipette
b. sterilize the pipette
c. adjust the volume
The Bradford assay only works at low protein concentrations from .05 2.5 mg/mL proteins.
How can I reduce the concentration of protein in our sample?
A.Evaporate the sample
B.Dilute the sample
C. Concentrate the sample
D. Purify the sample correct answers B. Dilute the sample
We will make a dilution series of our protein, to find a concentration that falls into the range
of measurable concentrations
Why are only sterile Pipette tips in the lab?
A. To prevent reproduction of proteins
B. To avoid contamination of the sample
C. To make sure that the tips can be reused
D. To make pipetting more precise correct answers B. To avoid contamination of the sample
(To sterilize pipette tips, they are heated to 120 Celsius under high pressure which kills most
germs
what is the upper limit of a p1000 pipette correct answers 1000 microliters
what is the lower limit of a pipette p1000 correct answers 200 micro-liters
What is the volume range of the P1000
a. 20-200 ul
b. 2-20 ul
c. .5-2 ul
d. 200-1000ul correct answers d.. 200-1000ul
What are the ranges for the 3 pipettes? correct answers P1000: 200-1000ul
P200: 20-200ul
P20: 2-20ul
How would you set the volume to pipette 20 ul with the P20
a. 200
b. 2
c. 20
d. 2000 correct answers a. 200
why did you press the plunger all the way down to the second stop?
a. to mix the contents in the tube
b. to push out the remaining fluid from the tip
c, to avoid contaminations
d. to draw up liquid correct answers b. to make sure that every drop of liquid is transferred to
the micro centrifuge to if we press the plunger to the second stop
What do you need to do next before pipetting a different sample?
a. discard the pipette
b. sterilize the pipette
c. adjust the volume
