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ASCP Blood Bank (Immunohematology) MLS Review 2025/2026 – 350+ High-Yield Questions with Explanations | ABO/Rh Typing, Antibody ID, Transfusion Reactions, HDFN, Compatibility Testing & More

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This ASCP Blood Bank Study Guide 2025/2026 is an in-depth, exam-focused resource containing 350+ multiple-choice questions with verified answers and explanations, built specifically for the Medical Laboratory Scientist (MLS) level of the ASCP BOC exam. It comprehensively covers all core and advanced topics in immunohematology, ensuring you're prepared for both routine blood bank procedures and complex serologic problem-solving. The material includes detailed coverage of: ABO/Rh blood grouping and discrepancies Antibody detection and identification including warm, cold, IgM, IgG, and clinically significant antibodies Transfusion reactions: recognition, testing protocols, and response strategies Hemolytic Disease of the Fetus and Newborn (HDFN): prenatal testing, RhIg administration, and fetal monitoring Compatibility testing and crossmatching procedures High-prevalence/low-prevalence antigens and advanced immunohematology panels Molecular testing and genotyping in blood bank practice Donor eligibility, component therapy, storage, and modifications (e.g., leukoreduction, irradiation) Explanations are provided to clarify not only the correct answers, but also why other options are incorrect—ideal for both content mastery and test-taking strategy. This study guide is perfect for: MLS (ASCP) exam candidates focusing on the blood bank section Clinical Laboratory Science and Medical Technology students ASCPi international applicants pursuing certification in immunohematology Laboratory professionals preparing for recertification or CE credits Anyone working in transfusion services, donor centers, or reference immunohematology labs Whether you're learning to interpret antibody panels or preparing for critical decision-making in transfusion reactions, this guide delivers a structured and exam-ready experience. Keywords: ASCP blood bank, immunohematology, antibody ID, ABO Rh typing, transfusion reaction, crossmatch, DAT, IAT, HDFN, RhIg, blood components, antigen phenotyping, weak D testing, elution, reference lab, Coombs test, MLS BOC exam, ASCP MLS 2025, transfusion services, compatibility testing

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ASCP MLS Blood Bank 2025/2026 Exam
Questions and Verified Answers |
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The minimum hemoglobin concentration in a fingerstick from a male blood

donor is:




a. 12.0 g/dL (120 g/L)

b. 12.5 g/dL (125 g/L)

c. 13.5 g/dL (135 g/L)


d. 15.0 g/dL (150 g/L) - 🧠ANSWER ✔✔b. All donors, regardless of sex,

require a minimum hemoglobin of 12.5 g/dL (125 g/L). The value must not

be performed on an earlobe stick.

A cause for permanent deferral of blood donation is:

,a. diabetes

b. residence in an endemic malaria region

c. history of jaundice of uncertain cause


d. history of therapeutic rabies vaccine - 🧠ANSWER ✔✔c. Jaundice is a

sign of liver impairment, which might be due to HBV or HCV. Infection with

HBV and HCV is a cause for indefinite deferral.

Which of the following prospective donors would be accepted for donation?




a. 32-year-old woman who received a transfusion in a complicated delivery

5 months previously




b. 19-year-old sailor who has been stateside for 9 months and stopped

taking his anti-malarial medication 9 months previously




c. 22-year-old college student who has a temperature of 99.2F (37.3C) and

states that he feels well, but is nervous about donating

,d. 45-year-old woman who has just recovered from a bladder infection and

is still taking antibiotics - 🧠ANSWER ✔✔c. The receipt of blood products is

a

6-month deferral, the deferral for travel to areas endemic for malaria is 12

months regardless of

antimalarial prophylaxis, and a person taking antibiotics may have

bacteremia. The requirement for temperature is not over 37.5°C or 99.5°F.

Which one of the following constitutes permanent rejection status of a

donor?




a. a tattoo 5 months previously

b. recent close contact with a patient with viral hepatitis

c. 2 units of blood transfused 4 months previously


d. confirmed positive test for HBsAg 10 years previously - 🧠ANSWER ✔✔d.

A positive test for HbsAg at any time is an indefinite deferral.

According to AABB standards, which of the following donors may be

accepted as a blood donor?



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, a. traveled to an area endemic for malaria 9 months previously

b. spontaneous abortion at 2 months of pregnancy, 3 months previously

c. resides with a known hepatitis patient


d. received a blood transfusion 22 weeks previously - 🧠ANSWER ✔✔b. A

woman who had a spontaneous abortion at 2 months of pregnancy, 3

months previously would be acceptable. A donor is acceptable if she has

not been pregnant in the previous 6 weeks.

Below are the results of the history obtained from a prospective female

blood donor:




Age: 16

Temperature: 99.0"F (37.2°C)

Hct: 36%

History: tetanus toxoid immunization 1 week previously

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