BIOD 171 Exam 3 Study UPDATED
ACTUAL Exam Questions and CORRECT
Answers
True or False: A nanometer (10-9) is longer than a micrometer (10-6). - CORRECT
ANSWER - False; A nanometer is 1,000 times smaller than a micrometer.
Resolution and contrast are two critical factors that influence your ability to see an object.
Explain each. - CORRECT ANSWER - Resolution is the difference in distance between
two objects that appear as two separate objects.
Contrast is the difference in light absorption by an object.
Assuming a constant (non-adjustable) light source power, identify the part of the microscope you
would adjust to limit the amount of light entering the microscope. - CORRECT
ANSWER - Iris diaphragm
What is the total magnification (relative to your eye) of a sample imaged with a 60x objective
and a 10x eyepiece? Show your math. - CORRECT ANSWER - 60 x 10 = 600x
magnification
True or False: Staining is often required to image a cell that is adherent and flat (thin). -
CORRECT ANSWER - True
Which of the following could NOT be seen clearly by the unaided eye? Select all that apply. -
CORRECT ANSWER - Bacteria with diameter of 24 μm
Virus with a diameter of 0.2 μm
This type of microscope utilizes ultraviolet (UV) light to illuminate stained objects. - CORRECT
ANSWER - Fluorescence
ACTUAL Exam Questions and CORRECT
Answers
True or False: A nanometer (10-9) is longer than a micrometer (10-6). - CORRECT
ANSWER - False; A nanometer is 1,000 times smaller than a micrometer.
Resolution and contrast are two critical factors that influence your ability to see an object.
Explain each. - CORRECT ANSWER - Resolution is the difference in distance between
two objects that appear as two separate objects.
Contrast is the difference in light absorption by an object.
Assuming a constant (non-adjustable) light source power, identify the part of the microscope you
would adjust to limit the amount of light entering the microscope. - CORRECT
ANSWER - Iris diaphragm
What is the total magnification (relative to your eye) of a sample imaged with a 60x objective
and a 10x eyepiece? Show your math. - CORRECT ANSWER - 60 x 10 = 600x
magnification
True or False: Staining is often required to image a cell that is adherent and flat (thin). -
CORRECT ANSWER - True
Which of the following could NOT be seen clearly by the unaided eye? Select all that apply. -
CORRECT ANSWER - Bacteria with diameter of 24 μm
Virus with a diameter of 0.2 μm
This type of microscope utilizes ultraviolet (UV) light to illuminate stained objects. - CORRECT
ANSWER - Fluorescence
