Rutgers Bio 117 final/ Rutgers bio lab final
UPDATED ACTUAL Exam Questions and
CORRECT Answers
Blast stands for - CORRECT ANSWER -Basic Local Alignment search tool
BOLD stands for - CORRECT ANSWER -Barcode of Life Database
What does DNA barcoding do - CORRECT ANSWER -use of a DNA Sequence or DNA
marker to identify a unknown species(or find new ones)
Conserved DNA/Conserved Genes - CORRECT ANSWER -Area of genes that show a
slow rate of change through out evolutionary history
Cytochrome C oxidase subunit 1 gene(COI) is the conserved gene for... - CORRECT
ANSWER -Animals
RBCL is the conserved gene for... - CORRECT ANSWER -plants
16s is the conserved gene for... - CORRECT ANSWER -bacteria
Why is COI gene used for DNA Barcoding of Animals - CORRECT ANSWER -it is fairly
similar with a certain species but contains DNA differences between species
What is GenBank - CORRECT ANSWER -the sequence data base at the National institute
of health
Sanger method/ Dideoxy chain termination sequencing - CORRECT ANSWER -1.
oligonucleotide primer binds to DNA template strand
, 2. DNA polymerase extends the strand in the 5' to 3' direction
4 the new strand is complementary to the template and contains the original dNTPS but also the
ddNTPS( which are missing a hydroxyl at the 3' end)
5. Where the ddNTPs are inserted is where the chains terminate and eventually with multiple
chains you will get every single size since the insertion of ddNTPs are random
6. they are then put in a gel and separated by size
Bioinformatic - CORRECT ANSWER -the application of computer science and IT
when viewing a wave from Ns represent - CORRECT ANSWER -mistakes and
ambiguities in the DNA
If the sequence quality is bad - CORRECT ANSWER -1. predict the problem
2. submit data
if the sequence good quality - CORRECT ANSWER -1. identify quality regions and crop
ends
2. search for DNA Matches(BLASTN)
3. compare mismatches and gaps
4. use barcode of life database to identify the organism
5. perform a multiple sequence alignment
finch tv uses green for... - CORRECT ANSWER -Adenine
finch tv uses red for... - CORRECT ANSWER -thymine
finch tv uses black for... - CORRECT ANSWER -guanine
finch tv uses blue for... - CORRECT ANSWER -cytosine
UPDATED ACTUAL Exam Questions and
CORRECT Answers
Blast stands for - CORRECT ANSWER -Basic Local Alignment search tool
BOLD stands for - CORRECT ANSWER -Barcode of Life Database
What does DNA barcoding do - CORRECT ANSWER -use of a DNA Sequence or DNA
marker to identify a unknown species(or find new ones)
Conserved DNA/Conserved Genes - CORRECT ANSWER -Area of genes that show a
slow rate of change through out evolutionary history
Cytochrome C oxidase subunit 1 gene(COI) is the conserved gene for... - CORRECT
ANSWER -Animals
RBCL is the conserved gene for... - CORRECT ANSWER -plants
16s is the conserved gene for... - CORRECT ANSWER -bacteria
Why is COI gene used for DNA Barcoding of Animals - CORRECT ANSWER -it is fairly
similar with a certain species but contains DNA differences between species
What is GenBank - CORRECT ANSWER -the sequence data base at the National institute
of health
Sanger method/ Dideoxy chain termination sequencing - CORRECT ANSWER -1.
oligonucleotide primer binds to DNA template strand
, 2. DNA polymerase extends the strand in the 5' to 3' direction
4 the new strand is complementary to the template and contains the original dNTPS but also the
ddNTPS( which are missing a hydroxyl at the 3' end)
5. Where the ddNTPs are inserted is where the chains terminate and eventually with multiple
chains you will get every single size since the insertion of ddNTPs are random
6. they are then put in a gel and separated by size
Bioinformatic - CORRECT ANSWER -the application of computer science and IT
when viewing a wave from Ns represent - CORRECT ANSWER -mistakes and
ambiguities in the DNA
If the sequence quality is bad - CORRECT ANSWER -1. predict the problem
2. submit data
if the sequence good quality - CORRECT ANSWER -1. identify quality regions and crop
ends
2. search for DNA Matches(BLASTN)
3. compare mismatches and gaps
4. use barcode of life database to identify the organism
5. perform a multiple sequence alignment
finch tv uses green for... - CORRECT ANSWER -Adenine
finch tv uses red for... - CORRECT ANSWER -thymine
finch tv uses black for... - CORRECT ANSWER -guanine
finch tv uses blue for... - CORRECT ANSWER -cytosine
