BCH 5413 EXAM 2 QUESTIONS AND CORRECT
DETAILED ANSWERS (VERIFIED ANSWERS) |
ALREADY GRADED A+ | LATEST UPDATE 2025-
2026
How many subunits are there in RNA pol II? - ANSWER 12
How were the subunits of RNA pol II identified? (list both methods) - ANSWER
1. 10 subunits of RNA pol II were identified using IP or cells treated with
radiolabeled sulfur. The complex was IP'd by pulling down a known component of
pol II that was epitope tagged. After IP the sample was run on SDS-PAGE 10 clear
bands were found. (Rpb 1 - 10 ID'd)
2. the total 12 subunits of RNA pol II were identified by IP without an epitope tage
(which made it cleaner) Rpb 1 - 12 were ID'd
What is the role of hRPB1? - ANSWER it contains the CTD and binds DNA
What is the role of hRPB2? - ANSWER it contains the active, ctls elongation
rate and helps with TSS selection
What is the role of hRPB3? - ANSWER interacts with Rbp11
What is the role of hRPB4? - ANSWER forms sub complex with Rbp7. Involved
in stress response
DETAILED ANSWERS (VERIFIED ANSWERS) |
ALREADY GRADED A+ | LATEST UPDATE 2025-
2026
How many subunits are there in RNA pol II? - ANSWER 12
How were the subunits of RNA pol II identified? (list both methods) - ANSWER
1. 10 subunits of RNA pol II were identified using IP or cells treated with
radiolabeled sulfur. The complex was IP'd by pulling down a known component of
pol II that was epitope tagged. After IP the sample was run on SDS-PAGE 10 clear
bands were found. (Rpb 1 - 10 ID'd)
2. the total 12 subunits of RNA pol II were identified by IP without an epitope tage
(which made it cleaner) Rpb 1 - 12 were ID'd
What is the role of hRPB1? - ANSWER it contains the CTD and binds DNA
What is the role of hRPB2? - ANSWER it contains the active, ctls elongation
rate and helps with TSS selection
What is the role of hRPB3? - ANSWER interacts with Rbp11
What is the role of hRPB4? - ANSWER forms sub complex with Rbp7. Involved
in stress response