CLM Exam 2: Immunology Testing
QUESTIONS AND CORRECT
DETAILED ANSWERS WITH
RATIONALES|AGRADE
What is serology testing? - CORRECT ANSWERS Involves the binding of specific Ab to the
corresponding Ag.
Results in either precipitation or agglutination.
What is precipitation vs agglutination - CORRECT ANSWERS Precipitation: Ag is a small, soluble
molecule
Agglutination: Ab are binding large particles.
How do we perform serology testing?
- Prozone
- Zone of equivalence
- Postzone - CORRECT ANSWERS - We fill 11 tubes with 1mL of patient's serum
- In each tube, we put differing levels of Ag
- In prozone, there is excess Ab
- In the zone of equivalence, there are optimal amounts of both Ab and Ag
- In postzone, there is excess Ag
, What are the applications of agglutination and hemagglutination? - CORRECT ANSWERS - Blood
typing
- Detecting Ab against bacteria causing infections (fourfold rise in titer)
What are the practical considerations of agglutination and hemagglutination? - CORRECT ANSWERS
- Easy
- Semi-quantitative
What is an agglutinin-titer? - CORRECT ANSWERS Reciprocal of the greatest serum dilution that
elicits positive agglutination reaction.
If an agglutination titer is <2 what does that mean? - CORRECT ANSWERS No reaction, no
detectable Ab.
As an agglutination titer increases, what does that mean? - CORRECT ANSWERS Number of
detectable Ab increases.
How can latex be used in agglutination studies? - CORRECT ANSWERS - Attach Ag to latex bead
- Attach Ab to latex bead
What does ELISA stand for? - CORRECT ANSWERS Enzyme-Linked Immunosorbent Assay
What is one type of ELISA? - CORRECT ANSWERS Sandwich ELISA
How does sandwich ELISA work? - CORRECT ANSWERS - 96 well plate with monoclonal Ab
permanently fixed to the sides
- Add patient's serum
- If Ag is present, it will bind
QUESTIONS AND CORRECT
DETAILED ANSWERS WITH
RATIONALES|AGRADE
What is serology testing? - CORRECT ANSWERS Involves the binding of specific Ab to the
corresponding Ag.
Results in either precipitation or agglutination.
What is precipitation vs agglutination - CORRECT ANSWERS Precipitation: Ag is a small, soluble
molecule
Agglutination: Ab are binding large particles.
How do we perform serology testing?
- Prozone
- Zone of equivalence
- Postzone - CORRECT ANSWERS - We fill 11 tubes with 1mL of patient's serum
- In each tube, we put differing levels of Ag
- In prozone, there is excess Ab
- In the zone of equivalence, there are optimal amounts of both Ab and Ag
- In postzone, there is excess Ag
, What are the applications of agglutination and hemagglutination? - CORRECT ANSWERS - Blood
typing
- Detecting Ab against bacteria causing infections (fourfold rise in titer)
What are the practical considerations of agglutination and hemagglutination? - CORRECT ANSWERS
- Easy
- Semi-quantitative
What is an agglutinin-titer? - CORRECT ANSWERS Reciprocal of the greatest serum dilution that
elicits positive agglutination reaction.
If an agglutination titer is <2 what does that mean? - CORRECT ANSWERS No reaction, no
detectable Ab.
As an agglutination titer increases, what does that mean? - CORRECT ANSWERS Number of
detectable Ab increases.
How can latex be used in agglutination studies? - CORRECT ANSWERS - Attach Ag to latex bead
- Attach Ab to latex bead
What does ELISA stand for? - CORRECT ANSWERS Enzyme-Linked Immunosorbent Assay
What is one type of ELISA? - CORRECT ANSWERS Sandwich ELISA
How does sandwich ELISA work? - CORRECT ANSWERS - 96 well plate with monoclonal Ab
permanently fixed to the sides
- Add patient's serum
- If Ag is present, it will bind
