GCD Exam 3
Which of the following may be used as a vector in a gene-cloning experiment?
a) mRNA
b) Plasmid
c) Virus
d) c & d - answer
The restriction enzymes used in gene-cloning experiments ___________, which
generates sticky ends that can _________.
a. cut the DNA, enter bacterial cells
b. cut the DNA, hydrogen bond with complementary sticky ends
c. methylate DNA, enter bacterial cells
d. methylate DNA, hydrogen bond with complementary sticky ends - answerb
Which of the following is the proper order of steps in a gene-cloning experiment
involving vectors?
1. Add DNA ligase
2. Incubate the chromosomal DNA and the vector DNA with a restriction enzyme.
3. Introduce the DNA into living cells
4. Mix the chromosomal DNA
a. 1,2,3,4
b. 2,3,1,4
c. 2,4,1,3
d. 1,2,4,3 - answerc
the function of reverse transcriptase is to:
a. copy RNA into DNA
b. copy DNA into RNA
c. translate RNA into proteins
d. translate DNA into protein - answera
In one PCR cycle, the correct order of steps is:
a. primer annealing, primer extension, denaturation
b. primer annealing, denaturation, primer extension
c. denaturation, primer annealing, primer extension
d. denaturation, primer extension, primer annealing - answerc
In reverse transcriptase PCR, the starting biological material is
a. chromosomal DNA
b. mRNA
c. proteins
, d. all of the above - answerb
During real time PCR, the synthesis of PCR products is analyzed
a. at the very end of the reaction by gel electrophoresis
b. at the very end of the reaction by fluorescence that is emitted within the thermocycler
c. during the PCR cycles by gel electrophoresis
d. during the PCR cycles by fluorescence that is emitted within the thermocycler -
answerd
When a dideoxyribonucleotide is incorporated into a growing strand,
a. the strand elongates faster
b. the strand cannot elongate
c. the stand becomes more susceptible to DNase I cleavage.
d. none of the above happens - answerb
Why is Taq polymerase used in PCR as opposed to DNA polymerase from another
source such as E. coli?
a. Taq polymerase makes DNA faster.
b. Taq polymerase is resistant to heat denaturation. Correct
c. Taq polymerase is less likely to make errors.
d. All of the above would be reasons for using Taq polymerase. - answerb
A primer used in dideoxy DNA sequencing is 20 nucleotides long and has the following
sequence: 5'-GGATCCATGACTAGTCCGAC-3'. A segment of DNA is cloned into a
vector and then the vector DNA is denatured and subjected to dideoxy DNA sequencing
method. Below is the DNA sequence from a region of the vector. The primer annealing
site is shown in bold and underlined.
3-CCCGATCGGCCTAGGTACTGATCAGGCTGAATGACT-5'
Based on the sequence above, what would be the size(s) of the band(s) (i.e., the
number of nucleotides in each band) in the lane in which dideoxyT had been added to
the sequencing reaction? - answer21, 22, 25
During dideoxy sequencing, dideoxy nucleotides - answera. can only be incorporated
into newly made DNA strands via Taq polymerase.
b. are incorporated into DNA strands but prevent further strand growth. Correct
c. are incorporated into DNA strands more easily than deoxy nucleotides.
d. cannot be incorporated into newly made DNA strands.
How do bacteria make insulin? - answer*the coding sequence of either A or B chain of
insulin is placed next to coding sequence of Ecoli's protein, Beta-galactosidase to create
a fusion protein (bc otherwise, they would be rapidly degraded by themselves in
bacteria cell)
*purified and treated iwth CNBr which cleaves B-galactosidase from A or B chain
Which of the following may be used as a vector in a gene-cloning experiment?
a) mRNA
b) Plasmid
c) Virus
d) c & d - answer
The restriction enzymes used in gene-cloning experiments ___________, which
generates sticky ends that can _________.
a. cut the DNA, enter bacterial cells
b. cut the DNA, hydrogen bond with complementary sticky ends
c. methylate DNA, enter bacterial cells
d. methylate DNA, hydrogen bond with complementary sticky ends - answerb
Which of the following is the proper order of steps in a gene-cloning experiment
involving vectors?
1. Add DNA ligase
2. Incubate the chromosomal DNA and the vector DNA with a restriction enzyme.
3. Introduce the DNA into living cells
4. Mix the chromosomal DNA
a. 1,2,3,4
b. 2,3,1,4
c. 2,4,1,3
d. 1,2,4,3 - answerc
the function of reverse transcriptase is to:
a. copy RNA into DNA
b. copy DNA into RNA
c. translate RNA into proteins
d. translate DNA into protein - answera
In one PCR cycle, the correct order of steps is:
a. primer annealing, primer extension, denaturation
b. primer annealing, denaturation, primer extension
c. denaturation, primer annealing, primer extension
d. denaturation, primer extension, primer annealing - answerc
In reverse transcriptase PCR, the starting biological material is
a. chromosomal DNA
b. mRNA
c. proteins
, d. all of the above - answerb
During real time PCR, the synthesis of PCR products is analyzed
a. at the very end of the reaction by gel electrophoresis
b. at the very end of the reaction by fluorescence that is emitted within the thermocycler
c. during the PCR cycles by gel electrophoresis
d. during the PCR cycles by fluorescence that is emitted within the thermocycler -
answerd
When a dideoxyribonucleotide is incorporated into a growing strand,
a. the strand elongates faster
b. the strand cannot elongate
c. the stand becomes more susceptible to DNase I cleavage.
d. none of the above happens - answerb
Why is Taq polymerase used in PCR as opposed to DNA polymerase from another
source such as E. coli?
a. Taq polymerase makes DNA faster.
b. Taq polymerase is resistant to heat denaturation. Correct
c. Taq polymerase is less likely to make errors.
d. All of the above would be reasons for using Taq polymerase. - answerb
A primer used in dideoxy DNA sequencing is 20 nucleotides long and has the following
sequence: 5'-GGATCCATGACTAGTCCGAC-3'. A segment of DNA is cloned into a
vector and then the vector DNA is denatured and subjected to dideoxy DNA sequencing
method. Below is the DNA sequence from a region of the vector. The primer annealing
site is shown in bold and underlined.
3-CCCGATCGGCCTAGGTACTGATCAGGCTGAATGACT-5'
Based on the sequence above, what would be the size(s) of the band(s) (i.e., the
number of nucleotides in each band) in the lane in which dideoxyT had been added to
the sequencing reaction? - answer21, 22, 25
During dideoxy sequencing, dideoxy nucleotides - answera. can only be incorporated
into newly made DNA strands via Taq polymerase.
b. are incorporated into DNA strands but prevent further strand growth. Correct
c. are incorporated into DNA strands more easily than deoxy nucleotides.
d. cannot be incorporated into newly made DNA strands.
How do bacteria make insulin? - answer*the coding sequence of either A or B chain of
insulin is placed next to coding sequence of Ecoli's protein, Beta-galactosidase to create
a fusion protein (bc otherwise, they would be rapidly degraded by themselves in
bacteria cell)
*purified and treated iwth CNBr which cleaves B-galactosidase from A or B chain
