Griffith's experiments with heat killed
pathogenic bacteria and live non-patho-
genic bacteria indicated something from When the heat-killed S cells were mixed
the dead bacteria could do something to with the living R cells they combined and
the living, non-pathogenic bacteria that formed living S cells- smooth encapsu-
he called transformation. What changes lated cells
were observed in colony appearance
and pathogenicity?
What biological macromolecule did Av-
ery, McCarty, and MacLeod identify as DNA
Griffith's transforming factor
Chromatin is composed of protein and
DNA. Likewise bacterial viruses (bacte-
riophage) are composed of DNA and
S-protein stays outside cells
protein. In the Hershey and Chase exper-
P-DNA enters cells
iments which of these two molecules en-
tered the bacterial cell and which stayed
on the outside?
In the structure of DNA, what part of DNA
synthesis (replication) was obvious from Semi-conservative
the double-stranded structure?
Replication is involved in which of the two
Hereditary Function
major DNA functions?
Transcription and translation are in-
volved in which of the two major DNA Cell Regulation Function
functions
1. Helicase
2. DNA polymerase III
Name the 5 enzymes needed for DNA
3. DNA polymerase I
replication
4. Primase
5. Ligase
Single-Stranded DNA Binding Protein =
Name the DNA binding protein
SSBP
1. Binds single-stranded DNA
What is the function of SSBP 2. Permits replication to copy single
strands
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, Portage learning genetics BIOD 210 module 5 exam questions and correct answers
1. Unwinds DNA
What is the function of Helicase
2. Permits DNA strand separation
What is the function of DNA polymerase 1. Replicates DNA
III 2. Synthesizes long strands of DNA
1. Repairs DNA
2. Cuts out mismatched DNA
What is the function of DNA polymerase 3. Cuts out RNA
I 4. Synthesizes short strands of DNA
5. Replaces RNA primer with DNA
6. Proof-reads DNA
Because they have two different func-
Why are two DNA polymerases neces-
tions and we need one for each direction
sary
of replication
1. Special RNA polymerase
What is the function of primase 2. Initiates DNA replication
3. Synthesizes short RNA primer
What is the function of ligase Repairs breaks in DNA strands
Why are 8 nucleotide triphosphates 4 are needed for RNA priming and 4 are
needed for DNA synthesis needed for DNA synthesis
At the 5' end of an Okazaki fragment,
Primase
what nucleotide polymer is present
At the 3' end of an Okazaki fragment,
DNA polymerase III
what nucleotide polymer is present
What enzyme converts Okazaki frag-
DNA polymerase I
ments in to exclusively DNA molecules
What enzyme links Okazaki fragments
Ligase
together
At the ori in a bacterial chromosome,
what is the region of replicating DNA
oriC
consisting of two replication forks, which
are moving in opposite directions
In a replication fork there are two tem-
Leading strand
plate strands, the leading strand and the
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