BIO 175 EXAM LAB PRACTICAL 1 QUESTIONS
WITH 100%RATED ANSWERS 2024/2025 LATEST
UPDATED/GET A+
Slide label - One side, in corner with name of bacteria, initials
Plate label - On side with media, not on bottom, with initials, date, class section and name of
bacteria
Inoculation - Producing a culture- intro of sample to nutrient media (agar) ex: TSA
Incubation - Inoculated sample placed in an incubator to encourage growth
Incubation temperature - 20-40 Celsius (because body temp is about 37)
Upside down (media side on top) - How plates are placed in incubator to prevent condensation
from affecting sample
5 I's - Inoculation
Incubation
Isolation
Inspection
Identification
Ocular lens - Multiplies the image from the objective lens by 10
Nosepiece - Revolving turret that houses objective lens
Objective lens - To lens closest to the specimen - 4x, 10x, 40x, 100x
Mechanical stage - Supports the specimen slide and holds it in place with the slide holder
Control lever - The opening in iris diaphragm that controls contrast
Substage condenser - Focuses the light from the light source
Fine focus knob - Fine tuning focus- only objective used for oil immersion
Coarse focus - Initial focusing- only focus control used with 4x
Light source - Light provides illumination of specimen
Coaxial stage controls - Used to move slide holder on mechanical stage- left-right, front-back
WITH 100%RATED ANSWERS 2024/2025 LATEST
UPDATED/GET A+
Slide label - One side, in corner with name of bacteria, initials
Plate label - On side with media, not on bottom, with initials, date, class section and name of
bacteria
Inoculation - Producing a culture- intro of sample to nutrient media (agar) ex: TSA
Incubation - Inoculated sample placed in an incubator to encourage growth
Incubation temperature - 20-40 Celsius (because body temp is about 37)
Upside down (media side on top) - How plates are placed in incubator to prevent condensation
from affecting sample
5 I's - Inoculation
Incubation
Isolation
Inspection
Identification
Ocular lens - Multiplies the image from the objective lens by 10
Nosepiece - Revolving turret that houses objective lens
Objective lens - To lens closest to the specimen - 4x, 10x, 40x, 100x
Mechanical stage - Supports the specimen slide and holds it in place with the slide holder
Control lever - The opening in iris diaphragm that controls contrast
Substage condenser - Focuses the light from the light source
Fine focus knob - Fine tuning focus- only objective used for oil immersion
Coarse focus - Initial focusing- only focus control used with 4x
Light source - Light provides illumination of specimen
Coaxial stage controls - Used to move slide holder on mechanical stage- left-right, front-back
