BIOD171 COMPLETE LATEST MDULE 4
BASED ACTUAL WITH QUESTIONS AND
ANSWERS
necessary for growth and survival - ANS-✅nutrients, temp, pH
CO2 and Light - ANS-✅photoautotroph
CO2 and Chemicals - ANS-✅chemoautotroph
Organic Compounds and Light - ANS-✅photoheterotroph
Organic Compounds and Chemicals - ANS-✅chemoheterotroph
obligate aerobes - ANS-✅require O2
settle at the top of test tube
Obligate anaerobes - ANS-✅killed by O2
settle at the bottom of test tube
faculative anaerobes - ANS-✅grows better with O2 but can live without O2
more concentrated at the top of test tube
nirtogen is a... - ANS-✅growth-inhibiting nutrient
trace elements - ANS-✅need in minuscule amounts to support metabolism
growth factors - ANS-✅organic chemicals needed in small quantities to support metabolism
ex: AA, cholesterol, heme, NADH
thermophile - ANS-✅HOT
>45 C
mesophile - ANS-✅medium
20-40 C
cause most human diseases and infections bc body temp is 37 C
psychophile - ANS-✅cold!!
<15 C
can survive refrigeration and spoil food
more H+ = more ___ - ANS-✅acidic
acidophile - ANS-✅0-6 pH
neutrophile - ANS-✅6.5-7.5 pH
alkalinophile - ANS-✅7-11.5 pH
inoculum - ANS-✅sample of the microbe
medium - ANS-✅liquid or solid
culture - ANS-✅microbes grown in the media or the process
colonies - ANS-✅cluster of cells arising from 1 parent cell
incubation period - ANS-✅the time microbes are being cultured
growth media - ANS-✅nutrient-rich broth for general purpose growth. defines or undefined.
selective media - ANS-✅grows specific bacteria while restricting others. does this by limiting nutrients,
varying pH or temp, or adding ingredients that inhibit the growth of specific bacteria
differential media - ANS-✅distinguishes between 2 microbes that are related
enriched media - ANS-✅used to grow fastidious microorganisms - organisms with complex
requirements such that if absent it will not grow
LB Agar - ANS-✅multi-purpose
non-selective
non-differential
used to grow E. Coli
BASED ACTUAL WITH QUESTIONS AND
ANSWERS
necessary for growth and survival - ANS-✅nutrients, temp, pH
CO2 and Light - ANS-✅photoautotroph
CO2 and Chemicals - ANS-✅chemoautotroph
Organic Compounds and Light - ANS-✅photoheterotroph
Organic Compounds and Chemicals - ANS-✅chemoheterotroph
obligate aerobes - ANS-✅require O2
settle at the top of test tube
Obligate anaerobes - ANS-✅killed by O2
settle at the bottom of test tube
faculative anaerobes - ANS-✅grows better with O2 but can live without O2
more concentrated at the top of test tube
nirtogen is a... - ANS-✅growth-inhibiting nutrient
trace elements - ANS-✅need in minuscule amounts to support metabolism
growth factors - ANS-✅organic chemicals needed in small quantities to support metabolism
ex: AA, cholesterol, heme, NADH
thermophile - ANS-✅HOT
>45 C
mesophile - ANS-✅medium
20-40 C
cause most human diseases and infections bc body temp is 37 C
psychophile - ANS-✅cold!!
<15 C
can survive refrigeration and spoil food
more H+ = more ___ - ANS-✅acidic
acidophile - ANS-✅0-6 pH
neutrophile - ANS-✅6.5-7.5 pH
alkalinophile - ANS-✅7-11.5 pH
inoculum - ANS-✅sample of the microbe
medium - ANS-✅liquid or solid
culture - ANS-✅microbes grown in the media or the process
colonies - ANS-✅cluster of cells arising from 1 parent cell
incubation period - ANS-✅the time microbes are being cultured
growth media - ANS-✅nutrient-rich broth for general purpose growth. defines or undefined.
selective media - ANS-✅grows specific bacteria while restricting others. does this by limiting nutrients,
varying pH or temp, or adding ingredients that inhibit the growth of specific bacteria
differential media - ANS-✅distinguishes between 2 microbes that are related
enriched media - ANS-✅used to grow fastidious microorganisms - organisms with complex
requirements such that if absent it will not grow
LB Agar - ANS-✅multi-purpose
non-selective
non-differential
used to grow E. Coli
