Portage Learning BIOD171 Microbiology Lab Exams 1-9

Portage Learning BIOD171
Microbiology Lab Exams 1-9
Answer the following questions
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1. What three elements are used in an autoclave to sterilize equipment?
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heat, pressure, and steam
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2. What is the minimum temperature an autoclave must be set at to achieve sterile
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m condition?


125°C


3. If you are working in a lab in which an autoclave is not available, and you
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are pressed for time,which would you chose to best sterilize your
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equipment? Hot steam or hot air? Explain why you chose your answer.
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Hot steam is the best choice as you can achieve a sterile environment in a matter
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m of minuteswhereas hot air will take several hours to achieve the same effect.
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4. What type of incubator is pictured below?
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Fixed incubator m

, Answer Key m




Answer the following questions.
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1. At what temperature is the fixed incubator set to, as presented in the lab video?
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37°C


2. At what temperature should you refrigerate bacterial samples? Explain why this is
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beneficial.
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4°C. This temperature slows bacterial growth and prolongs the life of the sample.
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3. What are the FOUR types of gloves presented in the lab video?
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Latex, Nitrile, Thermal cold, Thermal heat
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4. What THREE rules were discussed in regards to lab safety that would protect you
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and others from contamination?
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1. Never eat or drink in the lab
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2. Always wear appropriate PPE
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3. Never leave the lab wearing PPE
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5. What are the main sections that should be found in a lab notebook? Name at least 4.
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Objective, Procedure, Notes, Results and Deviations
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, Answer the following questions.
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1. You are a lab instructor and Sally Miller has turned in her lab notebook for you
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m to grade the lab experiment #1 on lab safety. Based upon what was covered in
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m the lab video, how should Sally haveentitled her experiment?
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SM01 Lab Safety m m




2. You arrive to your first day of work at a new lab. You are taking over for
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m someone who took a newjob at another lab. Your boss informs you that because
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m of time restraints, this person did not exactlyfollow the experimental protocol. In order
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m to proceed, you must know what he did differently. (1) According to the lab
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m lecture, under what section of his notebook would you look to find the experimental
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m steps? (2) As changes to the experimental steps were made, what are thesedifferences
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m called and how should it appear in the lab notebook?
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(1) Procedure—this is where the steps for the experimental protocol are recorded. m m m m m m m m m m




(2) Deviations. All deviations should be written in red to immediately
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m bring attention to thechanges in the protocol.
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LAB 2 m




1. Identify the part of the microscope indicated by the arrow.
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Oculars (or eyepiece) m m




2. Identify the part of the microscope indicated by the arrow.
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