Mlpao Exam Review Questions With
Complete Solutions
Source of blood for blood agar plates - Correct Ans-● 5% sheep's blood or rabbits blood
Length of lancets used for skin punctures - Correct Ans-● 2.4mm on infants: (birth-1 year) the
skin puncture should be performed on the planter surface of the heal.
● 2.0mm on children
● 3.1mm on adult: the skin puncture is performed on the finger.
Common stains in microbiology - Correct Ans-● Gram stain - crystal violet - grams iodine -
alcohol - safrinan
● Ziehl - nelson - acid fast bacilli - tuberculosis
● Albert's stain - corynebacteria
Growth requirements for microorganisms - Correct Ans-● Proper nutrition: Autotrophs (feed
on non-living) and Heterotroph (feed on living)
● Oxygen: Aerobes (need oxygen) and Anaerobes (do not need oxygen)
● Optimum temp: the temp at which it grows best
,● Darkness
● Moisture
● pH most are neutral 7
Response to finding clots in an anticoagulated blood tube - Correct Ans-● autoclavable box
Components found in transport media - Correct Ans-● Stuarts medium
● Amies medium - similar to Stewart's
● Trans grow - reg for Neisseria
● Components -
o sodium thioglycollate
o sodium glycerophosphate
o calcium chloride
o methylene blue
o agar
Specimen requirements in chemistry - Correct Ans-● specimens analyzed in clinical chemistry
include - blood, urine, CSF, synovial fluid, pleural fluid, Pericardial fluid, sweat
,Factors involved in the collections of a 24-hour urine specimen - Correct Ans-● Upon rising
in the morning urinate emptying your bladder completely. DO NOT collect this specimen and
note the exact time it occurred and write it down on the container
● Collect all urine voided after this time for 24 hours in the container provided by the physician
or lab. All urine passed during the 24-hour period must be saved
● Refrigerate the collected urine between all voiding's or keep in a cool place ● At exactly the
same time the following morning void completely again and add sample to collection container.
This completes your 24 hour collection
● Take the container to the lab as soon as possible, maintaining the cool temp in transit by
placing the specimen in a portable cooler or insulating bag.
Advantages of the vacuum tube system for phlebotomy - Correct Ans-● Blood goes directly
from the patient's vein into the appropriate test tube and to the right dept
● Best blood samples for analysis
● Fixative to tissue - 15 - 20 times the size of the tissue Correct Ans-- 15 - 20 times the size of
the tissue
● Clearing fluid e of the tissue Correct Ans-- 50 - 100 times the size
, ● Volume of wax Correct Ans-25 -50 times volume of tissue
Proper technique for opening blood tubes - Correct Ans-● To reduce aerosol open with
absorbent paper or towel
Parts of a pH meter - Correct Ans-o two electrodes contained within two glass tubes which are
both immersed in the test solution of unknown concentration
o the glass electrode contains a platinum wire in contact with silver chloride immersed in a dilute
hydrochloric acid solution contained in a thin bulb of soda lime glass. o The calomel reference
electrode contains a platinum wire in contact with mercury and calomel paste immersed in a
saturated potassium chloride solution
Tests that indicate an MI - Correct Ans-● Ck
● Ast
● Cholesterol
The effects of hepatitis on urine samples - Correct Ans-● Urine will be a dark yellow
Complete Solutions
Source of blood for blood agar plates - Correct Ans-● 5% sheep's blood or rabbits blood
Length of lancets used for skin punctures - Correct Ans-● 2.4mm on infants: (birth-1 year) the
skin puncture should be performed on the planter surface of the heal.
● 2.0mm on children
● 3.1mm on adult: the skin puncture is performed on the finger.
Common stains in microbiology - Correct Ans-● Gram stain - crystal violet - grams iodine -
alcohol - safrinan
● Ziehl - nelson - acid fast bacilli - tuberculosis
● Albert's stain - corynebacteria
Growth requirements for microorganisms - Correct Ans-● Proper nutrition: Autotrophs (feed
on non-living) and Heterotroph (feed on living)
● Oxygen: Aerobes (need oxygen) and Anaerobes (do not need oxygen)
● Optimum temp: the temp at which it grows best
,● Darkness
● Moisture
● pH most are neutral 7
Response to finding clots in an anticoagulated blood tube - Correct Ans-● autoclavable box
Components found in transport media - Correct Ans-● Stuarts medium
● Amies medium - similar to Stewart's
● Trans grow - reg for Neisseria
● Components -
o sodium thioglycollate
o sodium glycerophosphate
o calcium chloride
o methylene blue
o agar
Specimen requirements in chemistry - Correct Ans-● specimens analyzed in clinical chemistry
include - blood, urine, CSF, synovial fluid, pleural fluid, Pericardial fluid, sweat
,Factors involved in the collections of a 24-hour urine specimen - Correct Ans-● Upon rising
in the morning urinate emptying your bladder completely. DO NOT collect this specimen and
note the exact time it occurred and write it down on the container
● Collect all urine voided after this time for 24 hours in the container provided by the physician
or lab. All urine passed during the 24-hour period must be saved
● Refrigerate the collected urine between all voiding's or keep in a cool place ● At exactly the
same time the following morning void completely again and add sample to collection container.
This completes your 24 hour collection
● Take the container to the lab as soon as possible, maintaining the cool temp in transit by
placing the specimen in a portable cooler or insulating bag.
Advantages of the vacuum tube system for phlebotomy - Correct Ans-● Blood goes directly
from the patient's vein into the appropriate test tube and to the right dept
● Best blood samples for analysis
● Fixative to tissue - 15 - 20 times the size of the tissue Correct Ans-- 15 - 20 times the size of
the tissue
● Clearing fluid e of the tissue Correct Ans-- 50 - 100 times the size
, ● Volume of wax Correct Ans-25 -50 times volume of tissue
Proper technique for opening blood tubes - Correct Ans-● To reduce aerosol open with
absorbent paper or towel
Parts of a pH meter - Correct Ans-o two electrodes contained within two glass tubes which are
both immersed in the test solution of unknown concentration
o the glass electrode contains a platinum wire in contact with silver chloride immersed in a dilute
hydrochloric acid solution contained in a thin bulb of soda lime glass. o The calomel reference
electrode contains a platinum wire in contact with mercury and calomel paste immersed in a
saturated potassium chloride solution
Tests that indicate an MI - Correct Ans-● Ck
● Ast
● Cholesterol
The effects of hepatitis on urine samples - Correct Ans-● Urine will be a dark yellow
