Please check the examination details below before entering your candidate information
Candidate surname Other names
Centre Number Candidate Number
Pearson Edexcel Level 3 GCE
Afternoon (Time: 1 hour 45 minutes) Paper
reference 9BI0/01
Biology B 2025 Predicted Paper
Advanced
PAPER 1: Advanced Biochemistry, Microbiology and
Genetics
You must have: Total Marks
Scientific calculator, HB pencil, ruler
Instructions
•• Use black ink or ball‑point pen.
Fill in the boxes at the top of this page with your name,
centre number and candidate number.
•• Answer all questions.
Answer the questions in the spaces provided
– there may be more space than you need.
Information
•• The total mark for this paper is 90.
The marks for each question are shown in brackets
– use this as a guide as to how much time to spend on each question.
• structure
In question(s) marked with an asterisk (*), marks will be awarded for your ability to
your answer logically showing how the points that you make are related
or follow on from each other where appropriate.
Advice
•• Read each question carefully before you start to answer it.
Try to answer every question.
• Check your answers if you have time at the end.
, 2
1 Diagram below shows how protein synthesis happen
(a) Describe the formation of a peptide bond during protein synthesis, including the role of ribosomes
and tRNA. (2)
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
(b) Explain the differences between secondary and tertiary protein structures, and discuss the
significance of these structures for protein function. (3)
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
Edexcel AL Biology Question Paper 1B 2025 Predicted
, 3
(c) Describe how protein misfolding can lead to diseases such as Alzheimer’s, and explain the role of
chaperone proteins in preventing misfolding. (2)
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
(d) Explain the molecular mechanisms by which ribosomal RNA (rRNA) acts as a catalyst in peptide
bond formation. Why is rRNA considered a ribozyme? (2)
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
(Total for Question 1 = 10 marks)
Edexcel AL Biology Question Paper 1B 2025 Predicted
Candidate surname Other names
Centre Number Candidate Number
Pearson Edexcel Level 3 GCE
Afternoon (Time: 1 hour 45 minutes) Paper
reference 9BI0/01
Biology B 2025 Predicted Paper
Advanced
PAPER 1: Advanced Biochemistry, Microbiology and
Genetics
You must have: Total Marks
Scientific calculator, HB pencil, ruler
Instructions
•• Use black ink or ball‑point pen.
Fill in the boxes at the top of this page with your name,
centre number and candidate number.
•• Answer all questions.
Answer the questions in the spaces provided
– there may be more space than you need.
Information
•• The total mark for this paper is 90.
The marks for each question are shown in brackets
– use this as a guide as to how much time to spend on each question.
• structure
In question(s) marked with an asterisk (*), marks will be awarded for your ability to
your answer logically showing how the points that you make are related
or follow on from each other where appropriate.
Advice
•• Read each question carefully before you start to answer it.
Try to answer every question.
• Check your answers if you have time at the end.
, 2
1 Diagram below shows how protein synthesis happen
(a) Describe the formation of a peptide bond during protein synthesis, including the role of ribosomes
and tRNA. (2)
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
(b) Explain the differences between secondary and tertiary protein structures, and discuss the
significance of these structures for protein function. (3)
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
Edexcel AL Biology Question Paper 1B 2025 Predicted
, 3
(c) Describe how protein misfolding can lead to diseases such as Alzheimer’s, and explain the role of
chaperone proteins in preventing misfolding. (2)
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
(d) Explain the molecular mechanisms by which ribosomal RNA (rRNA) acts as a catalyst in peptide
bond formation. Why is rRNA considered a ribozyme? (2)
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
……………………………………………………………………………………………………………
(Total for Question 1 = 10 marks)
Edexcel AL Biology Question Paper 1B 2025 Predicted
