Hematology: Manual Cell Counting &
RBC indices (Exam 1)
What are the reagents used for a manual RBC count? - ✔️✔️Hayem's
Gower's
Isotonic saline (5.8% NaCl)
What are the physiological reasons for variations in white counts? - ✔️✔️1. Exercise
2. Stress and Anxiety
3. After eating
4. Race (AAs and Asians are slightly lower than Caucasians)
5. Time of day (WBC lower in morning)
What are the physiological reasons for variations in red cell counts? - ✔️✔️1. Age
2. Exercise
3. Gender
4. Altitude
5. Dehydration
6. Time of Day
What is the general formula for manual cell counting? - ✔️✔️#cells counted x dilution x
depth factor (10) / # of 1mm squares counted.
(this will equal the # of cells /mm^3 or uL)
What are the reagents used for manual WBC counts? - ✔️✔️THINK: I Always Have
Time for WBCs:
Acetic Acid
Hydrochloric Acid
Turks Diluting fluid (glacial acetic acid, aqueous gentian violet, distilled water)
How do you calculate dilutions for manual white or red blood cell counts? - ✔️✔️Total
volume/Blood volume = Dilution
Compare the dilutions that can be made with a white and red blood cell unopette
pipette: - ✔️✔️WBC-- 1:100
RBC-- 1:200
What are the normal ranges for adult and newborn hemoglobin and hematocrit levels? -
✔️✔️Hemoglobin
Males: 13-17g/dL
RBC indices (Exam 1)
What are the reagents used for a manual RBC count? - ✔️✔️Hayem's
Gower's
Isotonic saline (5.8% NaCl)
What are the physiological reasons for variations in white counts? - ✔️✔️1. Exercise
2. Stress and Anxiety
3. After eating
4. Race (AAs and Asians are slightly lower than Caucasians)
5. Time of day (WBC lower in morning)
What are the physiological reasons for variations in red cell counts? - ✔️✔️1. Age
2. Exercise
3. Gender
4. Altitude
5. Dehydration
6. Time of Day
What is the general formula for manual cell counting? - ✔️✔️#cells counted x dilution x
depth factor (10) / # of 1mm squares counted.
(this will equal the # of cells /mm^3 or uL)
What are the reagents used for manual WBC counts? - ✔️✔️THINK: I Always Have
Time for WBCs:
Acetic Acid
Hydrochloric Acid
Turks Diluting fluid (glacial acetic acid, aqueous gentian violet, distilled water)
How do you calculate dilutions for manual white or red blood cell counts? - ✔️✔️Total
volume/Blood volume = Dilution
Compare the dilutions that can be made with a white and red blood cell unopette
pipette: - ✔️✔️WBC-- 1:100
RBC-- 1:200
What are the normal ranges for adult and newborn hemoglobin and hematocrit levels? -
✔️✔️Hemoglobin
Males: 13-17g/dL
