BIO 4400 Cellular Physiology - Test 3 With Complete
Solutions 100% Verified!!
1. Recombinant DNA technologies involve techniques that permit the creation of
custom-made DNA molecules that can be introduced back into living organisms. These
technologies were first developed in the - ANSWER c. 1970s
2. Which of the following statements about restriction nucleases is FALSE? - ANSWER
b. Restriction nucleases recognize specific sequences on single-stranded DNA.
3. You have purified DNA from your recently deceased goldfish. Which of the following
restriction nucleases would you use if you wanted to end up with DNA fragments with an
average size of 70 kilobase pairs (kb) after complete digestion of the DNA? The
recognition sequence for each enzyme is indicated in the right-hand column. -
ANSWER d. Xzal GAAGGATCCTTC
You have a circular plasmid that can be cut by the restriction nuclease Hindlil, as
diagrammed in
Figure 10-4.
If you were to cut this circular piece of DNA with Hindl, which of the answers below best
predict what you would get? - ANSWER a. one linear piece of DNA
You have a piece of circular DNA that can be cut by the restriction nucleases Xhol and
Smal, as indicated in Figure 10-5.
Figure 10-5
If you were to cut this circular piece of DNA with both Xhol and Smal, how many
fragments of DNA would you end up with? - ANSWER b. 2
You have a piece of circular DNA that can be cut by the restriction nucleases EcoRI,
HindlI, and Notl, as indicated in Figure 10-6.
,Figure 10-6
Which of the following statements is FALSE? - ANSWER d. Two DNA fragments of
unequal size will be created when this DNA is cut by both Hindlil and EcoRI
7. You have a linear piece of DNA that can be cut by the restriction nucleases HindIll and
EcoRI, as diagrammed in Figure 10-7.
If you were to cut this linear DNA with HindIll, what type of DNA fragments do you
predict you would obtain? - ANSWER b. two linear pieces of DNA, only one of which
can be cut by EcoRI
During gel electrophoresis, DNA fragments - ANSWER a. travel through a matrix
containing a microscopic network of pores.
You are interested in a single-stranded DNA molecule that contains the following
sequence:
5' GATTGCAT.... -3'
Which molecule will hybridize to your sequence of interest? - ANSWER d.
5'-ATGCAATC-3'
During DNA renaturation, two DNA strands will - ANSWER c. re-form a double helix if
the two strands have complementary sequences.
"DNA renaturation" refers to the process by which two DNA strands will re-form a
double helix if the two strands are complementary, DNA strands should not renature if
their sequences are identical. The breaking of the hydrogen bonds between two DNA
strands is the process of denaturation.
DNA ligase is an enzyme used when making recombinant DNA molecules in the lab. In
what normal cellular process is DNA ligase involved? - ANSWER d. DNA replication
12. Figure 10-12 shows the cleavage sites of several restriction nucleases.
You cut a vector using the Pcil restriction nuclease. Which of the following restriction
, nucleases will generate a fragment that can be ligated into this cut vector with the
addition of only ligase and ATP? - ANSWER b. Ncol
However, you will not be able to excise the fragment after ligation, because you will
destroy both the Poil site and the Ncol site, creating a new site with the sequence
5'-ACATGG-3'
3'-TGTACC-5'
Figure 10-13 depicts a strategy by which a DNA fragment produced by cutting with the
EcoRI restriction nuclease can be joined to a DNA fragment produced by cutting DNA
with the Haelll restriction nuclease.
Note that cutting DNA with Ecol produces a staggered end, whereas cutting DNA with
Haelll produces a blunt end. Why must polymerase be added in this reaction? -
ANSWER a. Polymerase will fit in the staggered end to create a blunt end.
DNA can be introduced into bacteria by a mechanism called - ANSWER d.
transformation
a plasmid - ANSWER a. can confer antibiotic resistance to a bacterium
Which of the following statements about DNA libraries is TRUE? - ANSWER b. by
placing the library DNA into bacteria, the bacteria can be used to amplify the desired
DNA fragments from the DNA library.
Which of the following statements about genomic DNA libraries is FALSE? - ANSWER
c. the larger the genome of the organism from which a library is derived, the larger the
fragments inserted into the vector will tend to be.
A DNA library has been constructed by purifying chromosomal DNA from mice, cutting
the DNA with the restriction enzyme Notl, and inserting the fragments into the Notl site
of a plasmid vector. What information CANNOT be retrieved from this library? -
ANSWER c. sequences of the telomeres (the ends of the chromosomes)
Solutions 100% Verified!!
1. Recombinant DNA technologies involve techniques that permit the creation of
custom-made DNA molecules that can be introduced back into living organisms. These
technologies were first developed in the - ANSWER c. 1970s
2. Which of the following statements about restriction nucleases is FALSE? - ANSWER
b. Restriction nucleases recognize specific sequences on single-stranded DNA.
3. You have purified DNA from your recently deceased goldfish. Which of the following
restriction nucleases would you use if you wanted to end up with DNA fragments with an
average size of 70 kilobase pairs (kb) after complete digestion of the DNA? The
recognition sequence for each enzyme is indicated in the right-hand column. -
ANSWER d. Xzal GAAGGATCCTTC
You have a circular plasmid that can be cut by the restriction nuclease Hindlil, as
diagrammed in
Figure 10-4.
If you were to cut this circular piece of DNA with Hindl, which of the answers below best
predict what you would get? - ANSWER a. one linear piece of DNA
You have a piece of circular DNA that can be cut by the restriction nucleases Xhol and
Smal, as indicated in Figure 10-5.
Figure 10-5
If you were to cut this circular piece of DNA with both Xhol and Smal, how many
fragments of DNA would you end up with? - ANSWER b. 2
You have a piece of circular DNA that can be cut by the restriction nucleases EcoRI,
HindlI, and Notl, as indicated in Figure 10-6.
,Figure 10-6
Which of the following statements is FALSE? - ANSWER d. Two DNA fragments of
unequal size will be created when this DNA is cut by both Hindlil and EcoRI
7. You have a linear piece of DNA that can be cut by the restriction nucleases HindIll and
EcoRI, as diagrammed in Figure 10-7.
If you were to cut this linear DNA with HindIll, what type of DNA fragments do you
predict you would obtain? - ANSWER b. two linear pieces of DNA, only one of which
can be cut by EcoRI
During gel electrophoresis, DNA fragments - ANSWER a. travel through a matrix
containing a microscopic network of pores.
You are interested in a single-stranded DNA molecule that contains the following
sequence:
5' GATTGCAT.... -3'
Which molecule will hybridize to your sequence of interest? - ANSWER d.
5'-ATGCAATC-3'
During DNA renaturation, two DNA strands will - ANSWER c. re-form a double helix if
the two strands have complementary sequences.
"DNA renaturation" refers to the process by which two DNA strands will re-form a
double helix if the two strands are complementary, DNA strands should not renature if
their sequences are identical. The breaking of the hydrogen bonds between two DNA
strands is the process of denaturation.
DNA ligase is an enzyme used when making recombinant DNA molecules in the lab. In
what normal cellular process is DNA ligase involved? - ANSWER d. DNA replication
12. Figure 10-12 shows the cleavage sites of several restriction nucleases.
You cut a vector using the Pcil restriction nuclease. Which of the following restriction
, nucleases will generate a fragment that can be ligated into this cut vector with the
addition of only ligase and ATP? - ANSWER b. Ncol
However, you will not be able to excise the fragment after ligation, because you will
destroy both the Poil site and the Ncol site, creating a new site with the sequence
5'-ACATGG-3'
3'-TGTACC-5'
Figure 10-13 depicts a strategy by which a DNA fragment produced by cutting with the
EcoRI restriction nuclease can be joined to a DNA fragment produced by cutting DNA
with the Haelll restriction nuclease.
Note that cutting DNA with Ecol produces a staggered end, whereas cutting DNA with
Haelll produces a blunt end. Why must polymerase be added in this reaction? -
ANSWER a. Polymerase will fit in the staggered end to create a blunt end.
DNA can be introduced into bacteria by a mechanism called - ANSWER d.
transformation
a plasmid - ANSWER a. can confer antibiotic resistance to a bacterium
Which of the following statements about DNA libraries is TRUE? - ANSWER b. by
placing the library DNA into bacteria, the bacteria can be used to amplify the desired
DNA fragments from the DNA library.
Which of the following statements about genomic DNA libraries is FALSE? - ANSWER
c. the larger the genome of the organism from which a library is derived, the larger the
fragments inserted into the vector will tend to be.
A DNA library has been constructed by purifying chromosomal DNA from mice, cutting
the DNA with the restriction enzyme Notl, and inserting the fragments into the Notl site
of a plasmid vector. What information CANNOT be retrieved from this library? -
ANSWER c. sequences of the telomeres (the ends of the chromosomes)
