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Terms in this set (402)
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Consider the table below 12.42 ( CPI is calculated by multiplying all Paternity
where a child and a index tgt)
possible father (PF) share
the listed paternity
indices for each locus
listed (LOC-A1, LOC-B2,
LOC-C3, LOC-D4).
Locus Tested PF Child
Paternity Index
LOC-A1 3 2/3 2.18
LOC-B2 7/5 5 0.798
LOC-C3 15/17 9/17 5.21
LOC-D4 12 12 1.37
Based on the data
presented in the table,
what is the combined
paternity index, CPI, from
the loci tested: LOC-A1,
LOC-B2, LOC-C3 and
LOC-D4?
12.42
9.558
15.56
2.38
Best way to avoid PCR Separate Pre-PCR (clean area) and post PCR (dirty
contamination area)
A260=1.4, A280=1.1 . Protein contamination . A260/280 = 1.3
protein contamination or <1.6 = protein contamination, 1.7-2.0 = good DNA, 1.9-
Rna contam or useable 2.0 = good RNA
RNA
Why is Fluorometry a Increased sensitivity and more importantly can
better technique that accurately measure DNA concentration of a
Spectroscopy structurally intact double stranded DNA molecule
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LD is the non random association of alleles at 2 or
more loci in a general population. When alleles are
Linkage Disequilibrium
in LD, haplotypes do not occur at the expected
frequencies.
Specificity Specificity = TN / (TN + FP)
lowest concentration of analyte that can be
Limit of Detection (LOD)
detected
mRNA Sequence (Template/Anti-sense Strand) [Replace U with T]
provided - Convert it into
DNA
Correct order of HIV Plasma Preparation
Sample Preparation RNA Extraction
Workflow RNA Amplification/Modification Procedure
If a flask of cells is Trypsinize and/or scrape the cells inside the
provided for the provided flask, collect the cells suspended in the
intended purpose of media, spin, and then proceed further
DNA isolation, how
would you proceed
further
Which end of the primer 3'End of the Primer
is critical for the
increased specification of
PCR Amplification
process
Primer Dimer 5' End of the Primers (3-3' Was not an option)
Complementarity
Mechanism of Action of Altered Drug Target (PBP2A)
MecA Gene in MRSA
Technique to be used for Invader Assay (Non-Amplification Method)
FFPE Cervical Tissue
Provided
Liquid Based-Cytology HPV is the correct answer (Options Provided for:
Useful for what kind of HIV, HBV, HCV, HPV)
virus
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Isothermal Method that SDA (Strand Displacement Amplification)
utilizes Primes and
Polymerase to Create
Synthetic dsDNA
Polymerase used in BST (Bacillus Stearothermophilus)
Isothermal LAMP
Procedure
BRAF V600E Mutation is Lynch Syndrome (Also Known as HNPCC)
Associated with
CAP Requires At least 2 years from the date of discontinuation
Discontinued
SOP/Procedures to be
stored for how long
CAP Requires Laboratory Once a Year
Personnel (Technologist)
Proficiency Testing
Most Important Final Test Validation Summary Signed off by
Component of Clinical Laboratory Director
Laboratory Completed
Validation Studies
STR Electropherogram Two different alleles each was both recipient vs
provided and select the donor the most informative one
most informative loci
STR makes use of which Variable Nucleotide
of the following
Experiment Performed Insertion of New Restriction Site
(RFLP + PCR + Gel
Electrophoresis): three
bands observed in the
mutant sequence as
compared to the normal
sequence
Mutation in what gene UGT1A1
decreases metabolism of
Irinotecan Drug
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(Completed) MB (ASCP) 2025|Complete Exam
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Questions|Verified Solutions (100% correct)
Save
Terms in this set (402)
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,2/9/25, 1:48 PM (Completed) MB (ASCP) 2025|Complete Exam Test |Recently Testing Real Exam Questions|Verified Solutions (100% correct) F…
Consider the table below 12.42 ( CPI is calculated by multiplying all Paternity
where a child and a index tgt)
possible father (PF) share
the listed paternity
indices for each locus
listed (LOC-A1, LOC-B2,
LOC-C3, LOC-D4).
Locus Tested PF Child
Paternity Index
LOC-A1 3 2/3 2.18
LOC-B2 7/5 5 0.798
LOC-C3 15/17 9/17 5.21
LOC-D4 12 12 1.37
Based on the data
presented in the table,
what is the combined
paternity index, CPI, from
the loci tested: LOC-A1,
LOC-B2, LOC-C3 and
LOC-D4?
12.42
9.558
15.56
2.38
Best way to avoid PCR Separate Pre-PCR (clean area) and post PCR (dirty
contamination area)
A260=1.4, A280=1.1 . Protein contamination . A260/280 = 1.3
protein contamination or <1.6 = protein contamination, 1.7-2.0 = good DNA, 1.9-
Rna contam or useable 2.0 = good RNA
RNA
Why is Fluorometry a Increased sensitivity and more importantly can
better technique that accurately measure DNA concentration of a
Spectroscopy structurally intact double stranded DNA molecule
https://quizlet.com/1003454069/completed-mb-ascp-2025complete-exam-test-recently-testing-real-exam-questionsverified-solutions-100-correct… 2/74
,2/9/25, 1:48 PM (Completed) MB (ASCP) 2025|Complete Exam Test |Recently Testing Real Exam Questions|Verified Solutions (100% correct) F…
LD is the non random association of alleles at 2 or
more loci in a general population. When alleles are
Linkage Disequilibrium
in LD, haplotypes do not occur at the expected
frequencies.
Specificity Specificity = TN / (TN + FP)
lowest concentration of analyte that can be
Limit of Detection (LOD)
detected
mRNA Sequence (Template/Anti-sense Strand) [Replace U with T]
provided - Convert it into
DNA
Correct order of HIV Plasma Preparation
Sample Preparation RNA Extraction
Workflow RNA Amplification/Modification Procedure
If a flask of cells is Trypsinize and/or scrape the cells inside the
provided for the provided flask, collect the cells suspended in the
intended purpose of media, spin, and then proceed further
DNA isolation, how
would you proceed
further
Which end of the primer 3'End of the Primer
is critical for the
increased specification of
PCR Amplification
process
Primer Dimer 5' End of the Primers (3-3' Was not an option)
Complementarity
Mechanism of Action of Altered Drug Target (PBP2A)
MecA Gene in MRSA
Technique to be used for Invader Assay (Non-Amplification Method)
FFPE Cervical Tissue
Provided
Liquid Based-Cytology HPV is the correct answer (Options Provided for:
Useful for what kind of HIV, HBV, HCV, HPV)
virus
https://quizlet.com/1003454069/completed-mb-ascp-2025complete-exam-test-recently-testing-real-exam-questionsverified-solutions-100-correct… 3/74
, 2/9/25, 1:48 PM (Completed) MB (ASCP) 2025|Complete Exam Test |Recently Testing Real Exam Questions|Verified Solutions (100% correct) F…
Isothermal Method that SDA (Strand Displacement Amplification)
utilizes Primes and
Polymerase to Create
Synthetic dsDNA
Polymerase used in BST (Bacillus Stearothermophilus)
Isothermal LAMP
Procedure
BRAF V600E Mutation is Lynch Syndrome (Also Known as HNPCC)
Associated with
CAP Requires At least 2 years from the date of discontinuation
Discontinued
SOP/Procedures to be
stored for how long
CAP Requires Laboratory Once a Year
Personnel (Technologist)
Proficiency Testing
Most Important Final Test Validation Summary Signed off by
Component of Clinical Laboratory Director
Laboratory Completed
Validation Studies
STR Electropherogram Two different alleles each was both recipient vs
provided and select the donor the most informative one
most informative loci
STR makes use of which Variable Nucleotide
of the following
Experiment Performed Insertion of New Restriction Site
(RFLP + PCR + Gel
Electrophoresis): three
bands observed in the
mutant sequence as
compared to the normal
sequence
Mutation in what gene UGT1A1
decreases metabolism of
Irinotecan Drug
https://quizlet.com/1003454069/completed-mb-ascp-2025complete-exam-test-recently-testing-real-exam-questionsverified-solutions-100-correct… 4/74
