Bio final 425 lab review (latest update) questions with
complete solutions
1. 1. [CH-02-02] What
at least 60 percent
are concentrations
of ethanol or
propanol alcohol
required for hand
antiseptics?
at 100% the alcohol evaporates too fast before it can penetrate the
2. 2. [CH-02-03] Why cell wall.
isn't 100% ethanol
ef- fective against
bacte- ria?
3. 3. [CH-02-05] Is No, HIV is not resistant to disinfectants and antiseptics. HIV is highly
human sensitive to disinfectants and antiseptics as it is an enveloped
immunodefi- virus. If viral envelop becomes damaged, virus will no longer be
ciency virus (HIV) able to infect the host
re- sistant to
disinfec- tants and
antiseptics? Yes or
No. Explain your
answer?
Disinfectants are too harsh to be used on human skin and their
4. 4. [CH-02-07] What use is limited to unanimated objects only. If applied to human
is the difference skin, they will cause an
be-
tween antiseptics and irritation or even chemical burns. Antiseptics can be used on
human skin and
disinfectants? "magnification of microscope"
6. 6. [CH-03-03] Explain
5. 5. [CH-03-02] term "resolving pow- er of microscope"
Explain
term
1/
25
, Bio final 425 lab review (latest update) questions with
complete solutions
on unanimated objects. Both
disinfectants and antiseptics can
kill microbes.
Disinfectants are more likely to
do so
the ability of a microscope to
produce an image larger than its
actual size
defines how far apart two objects
must be in order to see them
separately
2/
25
, Bio final 425 lab review (latest update) questions with
complete solutions
7. 7. [CH-03-04] What Limit of microscope's resolution determines the minimal size of
is determined by object to be visible under microscope and minimal distance
the limit of between two objects to be "resolved" and be visible as two
microscope's distinct objects.
resolution?
8. 8. [CH-03-07] when one objective is in focus, the other will also be in focus
Explain
the term parfocal?
to avoid overcrowding of bacteria on the slide
9. 9. [CH-04-01] Why
is
it important to
make the
bacterial smear allows for the bacterial smear to stick to the slide.
thin?
10. 10. [CH-04-02]
What
is the purpose of
to prevent boiling/ killing the bacteria and altering the bacteria
heat-fixing the morphology
smear on the
slide?
11. 11. [CH-04-03] Why
is
it important to dry
the smear before
heat fix- ing?
12. 12. [CH-04-04] Why do basic dyes are positively charged and therefore attaches to the
negatively
methylene blue and at- tach to the bacterial cell?
other basic dyes
3/
25
complete solutions
1. 1. [CH-02-02] What
at least 60 percent
are concentrations
of ethanol or
propanol alcohol
required for hand
antiseptics?
at 100% the alcohol evaporates too fast before it can penetrate the
2. 2. [CH-02-03] Why cell wall.
isn't 100% ethanol
ef- fective against
bacte- ria?
3. 3. [CH-02-05] Is No, HIV is not resistant to disinfectants and antiseptics. HIV is highly
human sensitive to disinfectants and antiseptics as it is an enveloped
immunodefi- virus. If viral envelop becomes damaged, virus will no longer be
ciency virus (HIV) able to infect the host
re- sistant to
disinfec- tants and
antiseptics? Yes or
No. Explain your
answer?
Disinfectants are too harsh to be used on human skin and their
4. 4. [CH-02-07] What use is limited to unanimated objects only. If applied to human
is the difference skin, they will cause an
be-
tween antiseptics and irritation or even chemical burns. Antiseptics can be used on
human skin and
disinfectants? "magnification of microscope"
6. 6. [CH-03-03] Explain
5. 5. [CH-03-02] term "resolving pow- er of microscope"
Explain
term
1/
25
, Bio final 425 lab review (latest update) questions with
complete solutions
on unanimated objects. Both
disinfectants and antiseptics can
kill microbes.
Disinfectants are more likely to
do so
the ability of a microscope to
produce an image larger than its
actual size
defines how far apart two objects
must be in order to see them
separately
2/
25
, Bio final 425 lab review (latest update) questions with
complete solutions
7. 7. [CH-03-04] What Limit of microscope's resolution determines the minimal size of
is determined by object to be visible under microscope and minimal distance
the limit of between two objects to be "resolved" and be visible as two
microscope's distinct objects.
resolution?
8. 8. [CH-03-07] when one objective is in focus, the other will also be in focus
Explain
the term parfocal?
to avoid overcrowding of bacteria on the slide
9. 9. [CH-04-01] Why
is
it important to
make the
bacterial smear allows for the bacterial smear to stick to the slide.
thin?
10. 10. [CH-04-02]
What
is the purpose of
to prevent boiling/ killing the bacteria and altering the bacteria
heat-fixing the morphology
smear on the
slide?
11. 11. [CH-04-03] Why
is
it important to dry
the smear before
heat fix- ing?
12. 12. [CH-04-04] Why do basic dyes are positively charged and therefore attaches to the
negatively
methylene blue and at- tach to the bacterial cell?
other basic dyes
3/
25
