BIOL 2051 LAB FINAL questions with
verified solutions
proteins that serve as biological catalysts - correct answer ✔✔enzymes
substance that reacts with an enzyme - correct answer ✔✔substrate
generally, how many substrates may an enzyme react with? - correct answer
✔✔one
enzymes that act outside of the cell in which they are produced - correct answer
✔✔extracellular enzymes
Generally, how are extracellular enzymes useful? - correct answer ✔✔degrade
large molecules into units that are small enough to be transported by proteins
across the cell membrane and into the cell where they may be used
act inside of the cell to synthesize molecules that are needed by the cell OR
degrade molecules to simpler molecules, releasing energy - correct answer
✔✔endoenzymes
type of enzyme that is only produced when a substrate is available - correct
answer ✔✔inducible
type of enzyme that are essential to the cell and produced at all times - correct
answer ✔✔constitutive
,cell size, shape, and arrangement of a bacterium - correct answer ✔✔morphology
how an organism functions in an environment; its metabolic capabilities.. enzymes
produced and biochemical reactions - correct answer ✔✔physiology
What two enzymes are made by organisms that produce energy using aerobic
respiration? - correct answer ✔✔oxidase and catalase
in aerobic respiration, what serves as the final electron acceptor of the electron
transport chain - correct answer ✔✔molecular oxygen
oxygen accepts an electron from what protein in the electron transport chain
during aerobic respiration? - correct answer ✔✔cytochrome oxidase
What is tested for in an oxidase assay? - correct answer ✔✔cytochrome c oxidase
Many oxidase negative organisms are what in terms of oxygen use? - correct
answer ✔✔facultative aerobes
What are produced by the reduction of oxygen? - correct answer ✔✔toxic
byproducts
An organism's catalase production gives you information about what?
A) its optimal osmotic environment
B) Reduction of O2 during respiration
,C) the presence of nitrate reductase in the cell
D) fermentation properties - correct answer ✔✔B
T/F
a microscope slide is a convenient surface to perform a catalase test - correct
answer ✔✔true
Cytochrome oxidase is an enzyme at the end of
A) glycolysis
B) the electron transport chain
C) fermentation - correct answer ✔✔B
T/F
Dark-dier mutant of Chlamydomonas reinhardtii lacks a fundamental cytochrome
oxidase for aerobic respiration - correct answer ✔✔true
T/F
Dark-dier mutant of C. reinhardtii cannot use acetate in the dark for aerobic
respiration - correct answer ✔✔true
T/F
dark-dier mutant of C. reinhardtii can grow on minimal media - correct answer
✔✔true
T/F
, dark-dier mutant of C. reinhardtii is an anoxygenic phototroph - correct answer
✔✔false
Which of the following describes how you will inoculate an HA plate to test the
effects of mutation on oxidase function?
A) plate wild-type chlamys on one plate and dk mutant on another plate
B) divide the plate in half, placing wild type chlamy on one side and dk mutant on
the other side
C) streak plate
D) pour plate - correct answer ✔✔B
T/F
denitrification by soil microbes depletes the soil of nitrogen - correct answer
✔✔true
Which of the following are inoculated to test for nitrate reduction?
A) a nitrate broth with durham assembly
B) N-free broth
C) citrate slant
D) nitrite broth - correct answer ✔✔A
T/F
To perform the catalase test, add a drop of water to the slide to mix with the
bacteria - correct answer ✔✔false
verified solutions
proteins that serve as biological catalysts - correct answer ✔✔enzymes
substance that reacts with an enzyme - correct answer ✔✔substrate
generally, how many substrates may an enzyme react with? - correct answer
✔✔one
enzymes that act outside of the cell in which they are produced - correct answer
✔✔extracellular enzymes
Generally, how are extracellular enzymes useful? - correct answer ✔✔degrade
large molecules into units that are small enough to be transported by proteins
across the cell membrane and into the cell where they may be used
act inside of the cell to synthesize molecules that are needed by the cell OR
degrade molecules to simpler molecules, releasing energy - correct answer
✔✔endoenzymes
type of enzyme that is only produced when a substrate is available - correct
answer ✔✔inducible
type of enzyme that are essential to the cell and produced at all times - correct
answer ✔✔constitutive
,cell size, shape, and arrangement of a bacterium - correct answer ✔✔morphology
how an organism functions in an environment; its metabolic capabilities.. enzymes
produced and biochemical reactions - correct answer ✔✔physiology
What two enzymes are made by organisms that produce energy using aerobic
respiration? - correct answer ✔✔oxidase and catalase
in aerobic respiration, what serves as the final electron acceptor of the electron
transport chain - correct answer ✔✔molecular oxygen
oxygen accepts an electron from what protein in the electron transport chain
during aerobic respiration? - correct answer ✔✔cytochrome oxidase
What is tested for in an oxidase assay? - correct answer ✔✔cytochrome c oxidase
Many oxidase negative organisms are what in terms of oxygen use? - correct
answer ✔✔facultative aerobes
What are produced by the reduction of oxygen? - correct answer ✔✔toxic
byproducts
An organism's catalase production gives you information about what?
A) its optimal osmotic environment
B) Reduction of O2 during respiration
,C) the presence of nitrate reductase in the cell
D) fermentation properties - correct answer ✔✔B
T/F
a microscope slide is a convenient surface to perform a catalase test - correct
answer ✔✔true
Cytochrome oxidase is an enzyme at the end of
A) glycolysis
B) the electron transport chain
C) fermentation - correct answer ✔✔B
T/F
Dark-dier mutant of Chlamydomonas reinhardtii lacks a fundamental cytochrome
oxidase for aerobic respiration - correct answer ✔✔true
T/F
Dark-dier mutant of C. reinhardtii cannot use acetate in the dark for aerobic
respiration - correct answer ✔✔true
T/F
dark-dier mutant of C. reinhardtii can grow on minimal media - correct answer
✔✔true
T/F
, dark-dier mutant of C. reinhardtii is an anoxygenic phototroph - correct answer
✔✔false
Which of the following describes how you will inoculate an HA plate to test the
effects of mutation on oxidase function?
A) plate wild-type chlamys on one plate and dk mutant on another plate
B) divide the plate in half, placing wild type chlamy on one side and dk mutant on
the other side
C) streak plate
D) pour plate - correct answer ✔✔B
T/F
denitrification by soil microbes depletes the soil of nitrogen - correct answer
✔✔true
Which of the following are inoculated to test for nitrate reduction?
A) a nitrate broth with durham assembly
B) N-free broth
C) citrate slant
D) nitrite broth - correct answer ✔✔A
T/F
To perform the catalase test, add a drop of water to the slide to mix with the
bacteria - correct answer ✔✔false
