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MICROBIOLOGY LAB PRACTICAL 1 GACURA2024-2025 ACTUAL EXAM
COMPLETE 200-QUESTIONS AND CORRECT DETAILED ANSWERS
Quiz Primary containment
Ans: protection of the lab personnel and the lab environment
Quiz Secondary Containment
Ans: protection of the outside lab environment from exposure to infectious
organisms
Quiz Disposal of biohazards
Ans: Place biohazard material in marked bag or container; must be tagged or color
coded
Quiz Disposal of Glass waste
Ans: can be washed and reused after autoclaving (Kills station)
Quiz Disposal of plastic waste
Ans: Melt in autoclave to dispose after decontaminated
Quiz Office hours and location
Ans: Zurn 207 Monday/Wednesday 1-2:30
Quiz Cidal
Ans: killing
bactericidal
Quiz Static
, Ans: Halts growth
bacteriostatic
Quiz Resident Flora
Ans: microorganisms that normally reside on locations (like skin)
Hard to get rid of
Quiz transient flora
Ans: microbes that reside on the skin surface and are easily removed (not typical
residents on skin)- there from contamination
Quiz What type of agar do we use?
Ans: Tryptic Soy agar
Quiz Colony
Ans: visible cluster of microbes growing on solid media (1 cell asexually reproduces
until it's visible.
Quiz What temperature is an autoclave?
Ans: 121 degrees Celsius
Quiz What temp did we incubate the hand washing agar lab dish?
Ans: 37 degrees Celsius (human body temp)
Quiz Inoculation loop
Ans: Used to transfer microbiological live specimens under sterile conditions
Quiz Nutrient broth
Ans: liquid medium used to cultivate bacteria (it feeds bacteria)
Quiz What are the Eukaryotic super groups?
Ans: Unikonta
Excavata
Archaeplastida
SAR supergroup
Quiz Abbe Condenser