1
BIO
BIO 252 EXAM 3 QUESTIONS WITH CORRECT
VERIFIED SOLUTIONS 100% GUARANTEED
PASS (LATEST UPDATE)
What is the role of Dicer in RNA-induced gene silencing? - ANS ✓It cleaves
longer RNAs into short, regulatory RNA molecules. The regulatory siRNAs and
miRNAs are involved in suppression of gene expression.
T/F Alternative splicing of single mRNA transcripts gives rise to multiple
mRNAs. - ANS ✓True. Alternative splicing of mRNA transcripts is a process used
to regulate gene expression posttranscriptionally.
Pro or Eu:
adapted to challenges of a single-celled existence, rapid turnover of mRNA,
translation can occur immediately after mRNA is transcribed,cis-acting
regulatory proteins required for transcription initiation, regulation of
expression occurs at many checkpoints, chromatin remodeling required for
expression of many genes, introns in genes must be spliced out before
mRNA is mature, most organisms optimized for a multicellular lifestyle,
DNA found in nucleus requires transport across membranem, RNAs have
various half-lives - ANS ✓Pro: adapted to challenges of a single-celled existence,
rapid turnover of mRNA, translation can occur immediately after mRNA is
transcribed
Eu:
cis-acting regulatory proteins required for transcription initiation,
regulation of expression occurs at many checkpoints, chromatin
remodeling required for expression of many genes, introns in genes must
be spliced out before mRNA is mature, most organisms optimized for a
multicellular lifestyleDNA found in nucleus requires transport across
membrane, mRNAs have various half-lives
__________________ refers to changes in DNA/chromosome structure that can
influence overall gene output - ANS ✓chromatin remodeling
BIO 252
, 2
BIO
_________ are sequences in DNA to which transcription factors and RNA
polymerase bind to initiate transcription - ANS ✓promoters
____________ are cis- acting DNA sequences to which transcription factors bind
to regulate transcription. They can act over distances of thousands of base
pairs and can be upstream, downstream, or internal to the gene they affect.
- ANS ✓enhancers
cis- acting vs trans factors - ANS ✓
_____________ bind DNA and regulate transcription - ANS ✓transcription factor
The addition of a 5' cap and a poly A tail and removal of introns are all steps
in _________________ - ANS ✓RNA processing and transport
RNA processing contributes to ________________, which regulates translation -
ANS ✓mRNA stability
Which of the following clusters of terms applies when addressing
enhancers as elements associated with eukaryotic genetic regulation? - ANS
✓cis-acting, variable orientation, variable position
T/F Alternative RNA processing can result in different mRNAs that start
with different exons. - ANS ✓True
T/F Transcription in eukaryotes is generally influenced by enhancers, just
as in prokaryotes. - ANS ✓False
T/F Alternative RNA processing generates different mRNAs that can direct
the synthesis of different polypeptides. - ANS ✓True
What are transcription factors? - ANS ✓regulatory proteins that bind to
specific DNA sequences. Transcription factors are proteins that bind to specific
DNA recognition sequences in the promoter or enhancer elements of a gene.
Promoters vs. Enhancers:
may influence the expression of more than one gene, position can be
upstream, downstream, or within the gene,not required for basal-level
transcription, responsible for tissue- and time- specific gene
expression,always upstream of the gene within 100 bases of the
BIO 252
, 3
BIO
transcription initiation site, TATA, CAAT, GC boxes, required for basal-level
transcription - ANS ✓Promoters:
may influence the expression of more than one gene, position can be
upstream, downstream, or within the gene,not required for basal-level
transcription, responsible for tissue- and time- specific gene expression
Enhancers:
always upstream of the gene within 100 bases of the transcription
initiation site, TATA, CAAT, GC boxes, required for basal-level transcription
T/F Transcriptionally inactive regions of the genome are typically heavily
methylated. - ANS ✓True. Methylation of cytosine residues is often associated
with decreased transcription levels.
Mutagenic studies of the promoter region of the β-globin gene indicate that
mutations in some areas are more likely than mutations in other areas to
affect transcription rates. When mutations occur in consensus sequences
(modular elements such as GC box, CAAT box, TATA box), does
transcription increase or decrease? - ANS ✓decrease
For the genotype,
I + O+ Z+/ F' I + O+ Z+
which of the following should be entered into the chart?
Genotype
I + O+ Z+/ F' I + O+ Z+
I - Oc Z-/ F' I- Oc Z-
I - Oc Z+/ F' I - O+ Z+
I s Oc Z-/ F' I s O+ Z+
I + = wild-type repressor
I- = mutant repressor (unable to bind to the operator)
Is = mutant repressor (insensitive to lactose)
O+ = wild-type operator
Oc = constitutive operator (insensitive to repressor)
No Lactose/Lactose - ANS ✓- + (beta-gal made only when there is lactose)
For the genotype,
I- Oc Z-/ F' I- Oc Z-
which of the following should be entered into the chart?
Genotype
BIO 252
BIO
BIO 252 EXAM 3 QUESTIONS WITH CORRECT
VERIFIED SOLUTIONS 100% GUARANTEED
PASS (LATEST UPDATE)
What is the role of Dicer in RNA-induced gene silencing? - ANS ✓It cleaves
longer RNAs into short, regulatory RNA molecules. The regulatory siRNAs and
miRNAs are involved in suppression of gene expression.
T/F Alternative splicing of single mRNA transcripts gives rise to multiple
mRNAs. - ANS ✓True. Alternative splicing of mRNA transcripts is a process used
to regulate gene expression posttranscriptionally.
Pro or Eu:
adapted to challenges of a single-celled existence, rapid turnover of mRNA,
translation can occur immediately after mRNA is transcribed,cis-acting
regulatory proteins required for transcription initiation, regulation of
expression occurs at many checkpoints, chromatin remodeling required for
expression of many genes, introns in genes must be spliced out before
mRNA is mature, most organisms optimized for a multicellular lifestyle,
DNA found in nucleus requires transport across membranem, RNAs have
various half-lives - ANS ✓Pro: adapted to challenges of a single-celled existence,
rapid turnover of mRNA, translation can occur immediately after mRNA is
transcribed
Eu:
cis-acting regulatory proteins required for transcription initiation,
regulation of expression occurs at many checkpoints, chromatin
remodeling required for expression of many genes, introns in genes must
be spliced out before mRNA is mature, most organisms optimized for a
multicellular lifestyleDNA found in nucleus requires transport across
membrane, mRNAs have various half-lives
__________________ refers to changes in DNA/chromosome structure that can
influence overall gene output - ANS ✓chromatin remodeling
BIO 252
, 2
BIO
_________ are sequences in DNA to which transcription factors and RNA
polymerase bind to initiate transcription - ANS ✓promoters
____________ are cis- acting DNA sequences to which transcription factors bind
to regulate transcription. They can act over distances of thousands of base
pairs and can be upstream, downstream, or internal to the gene they affect.
- ANS ✓enhancers
cis- acting vs trans factors - ANS ✓
_____________ bind DNA and regulate transcription - ANS ✓transcription factor
The addition of a 5' cap and a poly A tail and removal of introns are all steps
in _________________ - ANS ✓RNA processing and transport
RNA processing contributes to ________________, which regulates translation -
ANS ✓mRNA stability
Which of the following clusters of terms applies when addressing
enhancers as elements associated with eukaryotic genetic regulation? - ANS
✓cis-acting, variable orientation, variable position
T/F Alternative RNA processing can result in different mRNAs that start
with different exons. - ANS ✓True
T/F Transcription in eukaryotes is generally influenced by enhancers, just
as in prokaryotes. - ANS ✓False
T/F Alternative RNA processing generates different mRNAs that can direct
the synthesis of different polypeptides. - ANS ✓True
What are transcription factors? - ANS ✓regulatory proteins that bind to
specific DNA sequences. Transcription factors are proteins that bind to specific
DNA recognition sequences in the promoter or enhancer elements of a gene.
Promoters vs. Enhancers:
may influence the expression of more than one gene, position can be
upstream, downstream, or within the gene,not required for basal-level
transcription, responsible for tissue- and time- specific gene
expression,always upstream of the gene within 100 bases of the
BIO 252
, 3
BIO
transcription initiation site, TATA, CAAT, GC boxes, required for basal-level
transcription - ANS ✓Promoters:
may influence the expression of more than one gene, position can be
upstream, downstream, or within the gene,not required for basal-level
transcription, responsible for tissue- and time- specific gene expression
Enhancers:
always upstream of the gene within 100 bases of the transcription
initiation site, TATA, CAAT, GC boxes, required for basal-level transcription
T/F Transcriptionally inactive regions of the genome are typically heavily
methylated. - ANS ✓True. Methylation of cytosine residues is often associated
with decreased transcription levels.
Mutagenic studies of the promoter region of the β-globin gene indicate that
mutations in some areas are more likely than mutations in other areas to
affect transcription rates. When mutations occur in consensus sequences
(modular elements such as GC box, CAAT box, TATA box), does
transcription increase or decrease? - ANS ✓decrease
For the genotype,
I + O+ Z+/ F' I + O+ Z+
which of the following should be entered into the chart?
Genotype
I + O+ Z+/ F' I + O+ Z+
I - Oc Z-/ F' I- Oc Z-
I - Oc Z+/ F' I - O+ Z+
I s Oc Z-/ F' I s O+ Z+
I + = wild-type repressor
I- = mutant repressor (unable to bind to the operator)
Is = mutant repressor (insensitive to lactose)
O+ = wild-type operator
Oc = constitutive operator (insensitive to repressor)
No Lactose/Lactose - ANS ✓- + (beta-gal made only when there is lactose)
For the genotype,
I- Oc Z-/ F' I- Oc Z-
which of the following should be entered into the chart?
Genotype
BIO 252
