ASCP CG Certification Practice With Complete
Solutions 100%
When harvesting peripheral blood cultures, asceptic technique no longer matters
following incubation with: - ANSWER -Colcemid - mitotic spindle poison that
halts cells at metaphase
Samples that arrive at the cytogenetics lab should first be: - ANSWER -Logged in
to the system
Phytohemagglutinin (PHA) stimulates which type of cells into division? -
ANSWER -T-cell lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a culture
failure, what is the MOST important to document? - ANSWER -Expiration dates
of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus)
sample? - ANSWER -Dissection
Which cell type is most problematic if found in a CVS (chorionic villus) sample? -
ANSWER -Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a lot of cytoplasm is still
seen around the mets, what is the most likely cause? - ANSWER -innappropriate
slide drying time
What will help prevent microbial contamination and cross contamination of
cultures? - ANSWER -Asceptic technique
When there are not enough mets to complete the study of a patient/case, what
needs to be done next? - ANSWER -prepare additional slides from any remaining
sample
, When looking at a dropped slide under a phase scope and mets are very spread out
and sparse, what could help fix the issue? - ANSWER -Increading the drying time
by using warm slides
What is true of an inverted microscope? - ANSWER -the objectives are beneath
the stage
High-resolution banding is indicated for a possible diagnosis of: - ANSWER -
DiGenorge syndrome -- microdeletion of 22q11, also called velocardiofacial
syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular
nose, wide spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what is the
MOST important data to accompany the specimen? - ANSWER -Reason for
referral
Which type of specimen should be requested for a cytogenetic analysis for turner
syndrome? - ANSWER -Heparinized peripheral blood stored at room temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a malignancy only if: -
ANSWER -Dividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator are
important to monitor to assess the source of the problem? - ANSWER -
Temperature,
CO2 level, and
Humidity level
, The most important factor affecting the quality of chromosomes viewed under a
phase contrast scope is: - ANSWER -Slide drying after the cells have been dropped
What is the modal number (default number of chromosomes in a cell) for a Down
syndrome translocation carrier? - ANSWER -45
What is the best tissue to use for both a good success rate and abnormality
detection in spontaneous abortion miscarriages? - ANSWER -Chorionic villi
What is a possible cause for a drying oven to not maintain a proper temperature? -
ANSWER -The door does not seal properly
If a tech with a cold and sneezing does not take proper precautions while setting
up amniotic fluid samples, the specimen could become contaminated with: -
ANSWER -Mycoplasma
--> the most common contaminant by a sick tech and can be detected through a
specific stain used on the sample
mycoplasma can induce abnormalities in a sample, so it is important to avoid
contamination
What about the metaphases on a slide are affected by slide making? - ANSWER -
Cell density - thru dilution of the sample with fix
Banding - based on how dark/pale/refractive they are affects trypsin time
Chromosome spreading - affected by drying time
What test(s) should be ordered on a newborn with suspected Prader-Willi
syndrome? - ANSWER -High resolution banding and FISH for the Prader-Willi
locus on 15q11.2
Solutions 100%
When harvesting peripheral blood cultures, asceptic technique no longer matters
following incubation with: - ANSWER -Colcemid - mitotic spindle poison that
halts cells at metaphase
Samples that arrive at the cytogenetics lab should first be: - ANSWER -Logged in
to the system
Phytohemagglutinin (PHA) stimulates which type of cells into division? -
ANSWER -T-cell lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a culture
failure, what is the MOST important to document? - ANSWER -Expiration dates
of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus)
sample? - ANSWER -Dissection
Which cell type is most problematic if found in a CVS (chorionic villus) sample? -
ANSWER -Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a lot of cytoplasm is still
seen around the mets, what is the most likely cause? - ANSWER -innappropriate
slide drying time
What will help prevent microbial contamination and cross contamination of
cultures? - ANSWER -Asceptic technique
When there are not enough mets to complete the study of a patient/case, what
needs to be done next? - ANSWER -prepare additional slides from any remaining
sample
, When looking at a dropped slide under a phase scope and mets are very spread out
and sparse, what could help fix the issue? - ANSWER -Increading the drying time
by using warm slides
What is true of an inverted microscope? - ANSWER -the objectives are beneath
the stage
High-resolution banding is indicated for a possible diagnosis of: - ANSWER -
DiGenorge syndrome -- microdeletion of 22q11, also called velocardiofacial
syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular
nose, wide spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic analysis, what is the
MOST important data to accompany the specimen? - ANSWER -Reason for
referral
Which type of specimen should be requested for a cytogenetic analysis for turner
syndrome? - ANSWER -Heparinized peripheral blood stored at room temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a malignancy only if: -
ANSWER -Dividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator are
important to monitor to assess the source of the problem? - ANSWER -
Temperature,
CO2 level, and
Humidity level
, The most important factor affecting the quality of chromosomes viewed under a
phase contrast scope is: - ANSWER -Slide drying after the cells have been dropped
What is the modal number (default number of chromosomes in a cell) for a Down
syndrome translocation carrier? - ANSWER -45
What is the best tissue to use for both a good success rate and abnormality
detection in spontaneous abortion miscarriages? - ANSWER -Chorionic villi
What is a possible cause for a drying oven to not maintain a proper temperature? -
ANSWER -The door does not seal properly
If a tech with a cold and sneezing does not take proper precautions while setting
up amniotic fluid samples, the specimen could become contaminated with: -
ANSWER -Mycoplasma
--> the most common contaminant by a sick tech and can be detected through a
specific stain used on the sample
mycoplasma can induce abnormalities in a sample, so it is important to avoid
contamination
What about the metaphases on a slide are affected by slide making? - ANSWER -
Cell density - thru dilution of the sample with fix
Banding - based on how dark/pale/refractive they are affects trypsin time
Chromosome spreading - affected by drying time
What test(s) should be ordered on a newborn with suspected Prader-Willi
syndrome? - ANSWER -High resolution banding and FISH for the Prader-Willi
locus on 15q11.2
