Biol 225 Uvic Midterm 1 Exam Questions
With Solved Solutions.
what is a cell - Answer vessel that contains the hereditary information of the species and the
machinery necessary for growth and reproduction
schleiden and schwann doctrines - Answer 1. all life forms are made from one or more cells
2. the cell is the basic unit of structure for all organisms
virchow doctrine - Answer 3. all cells arise from pre-existing cells
10 universal features of cells - Answer 1. all cells store hereditary info as DNA
2. all cells replicate DNA by semi-conservative replication
3. all cells transcribe their info as RNA
4. all cells use proteins as catalysts
5. all cells translate RNA into protein the same way
6. the fragment of info corresponding to one protein is one gene
7. life requires free energy
8. all cells function as biochemical factories with the same building blocks
9. all cells are enclosed in the plasma membrane
10. a living cell can exist with fewer than 500 genes
cell biology involves three genres - Answer biochemistry, genetics, cytology
green fluourescent protein - Answer can be inserted into receptor gene or made into fusion protein
reporter genes - Answer placed behind regulatory sequence for a specific protein and it quantifies the
amount of that protein thats being made
, fusion protein - Answer synthesize the same protein but with gfp attached
why are cells so small - Answer sa/v ratio
limiting for exchange with environment, diffusion time, RNA production
prokaryotes vs eukaryotes - Answer prok: lack membrane bound organelles
euks: true nucleus, highly compartmentalized, well developed cytoskeleton
advantages to multicellularity - Answer increase complexity and size
cell culture - Answer cells grown outside of the organism
primary culture - Answer tissue taken directly from organism
secondary culture - Answer primary culture growth taken and replated
mass culture - Answer large population of cells, grown to confluence
clonal culture - Answer known genetic lineage
axenic culture - Answer only one species
cells typically grown in: - Answer - suspended in liquid (microbes or transformed cells)
- semi-solid (transformed cells)
- plated (human cells wont grow on agar, need ECM)
normal cell - Answer -contact inhibited
-anchorage dependent
-growth factor dependent
With Solved Solutions.
what is a cell - Answer vessel that contains the hereditary information of the species and the
machinery necessary for growth and reproduction
schleiden and schwann doctrines - Answer 1. all life forms are made from one or more cells
2. the cell is the basic unit of structure for all organisms
virchow doctrine - Answer 3. all cells arise from pre-existing cells
10 universal features of cells - Answer 1. all cells store hereditary info as DNA
2. all cells replicate DNA by semi-conservative replication
3. all cells transcribe their info as RNA
4. all cells use proteins as catalysts
5. all cells translate RNA into protein the same way
6. the fragment of info corresponding to one protein is one gene
7. life requires free energy
8. all cells function as biochemical factories with the same building blocks
9. all cells are enclosed in the plasma membrane
10. a living cell can exist with fewer than 500 genes
cell biology involves three genres - Answer biochemistry, genetics, cytology
green fluourescent protein - Answer can be inserted into receptor gene or made into fusion protein
reporter genes - Answer placed behind regulatory sequence for a specific protein and it quantifies the
amount of that protein thats being made
, fusion protein - Answer synthesize the same protein but with gfp attached
why are cells so small - Answer sa/v ratio
limiting for exchange with environment, diffusion time, RNA production
prokaryotes vs eukaryotes - Answer prok: lack membrane bound organelles
euks: true nucleus, highly compartmentalized, well developed cytoskeleton
advantages to multicellularity - Answer increase complexity and size
cell culture - Answer cells grown outside of the organism
primary culture - Answer tissue taken directly from organism
secondary culture - Answer primary culture growth taken and replated
mass culture - Answer large population of cells, grown to confluence
clonal culture - Answer known genetic lineage
axenic culture - Answer only one species
cells typically grown in: - Answer - suspended in liquid (microbes or transformed cells)
- semi-solid (transformed cells)
- plated (human cells wont grow on agar, need ECM)
normal cell - Answer -contact inhibited
-anchorage dependent
-growth factor dependent
