BIO 351 QUIZ 2 – EXAM PREP 1
You have made a targeting vector to knock out Gene X. What is the next step? -
ANSWER Electroporate the vector into embryonic stem cells (ES) and select
those that are correctly targeted.
You are making a knockout mouse, after neomycin (positive) selection you treat
the surviving cells with gancyclovir (negative selection) and more cells die.
What does cell death after gancyclovir treatment indicate? - ANSWER The
targeting vector randomly inserted into the genome
Which of the following site-specific recombinases are useful for conditional
mutagenesis? - ANSWER Cre/loxp
Dre/rox
flp/FRT
When making a transgenic mouse we take advantage of which of the following
processes to insert the transgene into the genome? - ANSWER
nonhomologous recombination
You have a mouse with a floxed gene X allele and you want to knock this out in
the brain only. Which of the following would you do? - ANSWER Breed this
to a mouse that expresses Cre recombinase driven by a brain specific promoter.
The following figure shows gene expression of MyoD, an important
transcription factor involved in myogenesis, at various embryonic mouse stages
as detected by an antisense mRNA probe.
Which of the following techniques was used to generate these data? -
ANSWER In situ hybridization (ISH)
Which of the following is the correct order of developmental steps leading to a
specialized cell type? - ANSWER specification, determination, differentiation
Which of the following describes the minimum components of a transgene? -
ANSWER It has a promoter, an intron, a protein coding sequence (reporter
gene) and transcriptional STOP
You have made a targeting vector to knock out Gene X. What is the next step? -
ANSWER Electroporate the vector into embryonic stem cells (ES) and select
those that are correctly targeted.
You are making a knockout mouse, after neomycin (positive) selection you treat
the surviving cells with gancyclovir (negative selection) and more cells die.
What does cell death after gancyclovir treatment indicate? - ANSWER The
targeting vector randomly inserted into the genome
Which of the following site-specific recombinases are useful for conditional
mutagenesis? - ANSWER Cre/loxp
Dre/rox
flp/FRT
When making a transgenic mouse we take advantage of which of the following
processes to insert the transgene into the genome? - ANSWER
nonhomologous recombination
You have a mouse with a floxed gene X allele and you want to knock this out in
the brain only. Which of the following would you do? - ANSWER Breed this
to a mouse that expresses Cre recombinase driven by a brain specific promoter.
The following figure shows gene expression of MyoD, an important
transcription factor involved in myogenesis, at various embryonic mouse stages
as detected by an antisense mRNA probe.
Which of the following techniques was used to generate these data? -
ANSWER In situ hybridization (ISH)
Which of the following is the correct order of developmental steps leading to a
specialized cell type? - ANSWER specification, determination, differentiation
Which of the following describes the minimum components of a transgene? -
ANSWER It has a promoter, an intron, a protein coding sequence (reporter
gene) and transcriptional STOP
