Microbiology Lab final| exam test

Microbiology Lab final| exam test Microbiology Lab final| exam test aseptic technique - correct answer-A procedure performed under sterile conditions. any technique designed to keep unwanted microorganisms from contaminating sterile materials. Transfer loop - correct answer-also called an inoculating loop or needle is used to transfer microbial cultures. A bacterial mass equal to the size of a pinhead contains how many microorganisms - correct answer-several million How much of a culture is necessary to get on the inoculating loop? - correct answer-very small or barely visible quantities are sufficient To prevent exposure to air contamination what should you do to the plate? - correct answer-covers on the plates should always be closed Function of compound microscope - correct answer-uses a series of magnifying lenses to increase the size and illuminate very small specimens like bacterial cells, fungi, and protozoa that are unable to be seen with the naked eye. lens magnification range - correct answer-40x-1000x What is the magnification power of the ocular eyepiece of a compound microscope? - correct answer-10x What is magnification power of the oil immersion lens? - correct answer-100x total magnification - correct answer-objective lens x ocular lens Scan TM - correct answer-4 x 10 Low Power TM - correct answer-10 x 10 High dry power TM - correct answer-40 x 10 Oil immersion TM - correct answer-100 x 10 high dry power vs low power - correct answer-high dry power is used for smaller objects and uses fine focus lens. Lowe power is used for larger objects and uses coarse focus lens. how does loss of light affect the resolution - correct answer-it decreases the resolution or clarity what is the purpose of immersion oil - correct answer-to keep the light from refracting and increase the resolution base - correct answer-used in carrying the microscope and houses the light source. light source - correct answer-provides illumination to specimen Arm - correct answer-along with base, used as a carrying handle for microscope stage - correct answer-platform which the specimen rests on Coarse and fine focus adjustment knobs - correct answer-controls the distance between the specimen and the stage Coarse focus adjustment - correct answer-is used with scan and low power objective lenses Fine focus adjustment - correct answer-used with high dry and oil immersion objective lenses mechanical stage adjusters - correct answer-allows the movement of the stage from left to right and forward to backward condenser - correct answer-collects and concentrates light emitted from the light sources into a tight beam before passing it through the specimen iris diaphragm - correct answer-located wit