C785 Final BIOCHEM Exam Questions
and Answers
Which mRNA sequence results in a polypeptide that contains three amino acids? - Answer-5' - CUU GAU
ACU UAG - 3'
Which RNA sequence would end in the following polypeptide?
Phe-Thr-Val-Stop - Answer-5' - UUC ACA GUA UAA - 3'
What is the complementary sequence for the following DNA sequence?
5' - GCC ATC TCG AAT - 3' - Answer-5' - ATT CGA GAT GGC - 3'
Assuming 100% reaction efficiency, how many DNA copies are created after the completion of four
complete PCR cycles? - Answer-16
What is the function of DNA polymerase in the process of PCR? - Answer-It recognizes the primers and
uses the available dNTPs to replicate the template DNA sequence.
, 5' - [...] ATT CGC TAG GGC [...] - 3' − Normal DNA
5' - [...] ATT CGC GAG GGC [...] - 3' − Mutant DNA
Which type of mutation and outcome characterizes the Mutant DNA? - Answer-A missense mutation
leading to a longer protein
In sickle-cell disease, the glutamic acid (Glu) at position number seven is mutated to valine (Val).
What change in codons corresponds to this mutation? - Answer-GAA to GTA
What are possible blood types of the parents of a patient with blood type AB? - Answer-A and B
Which pair of chromosomes accurately depicts the genotype of a person with an autosomal recessive
mutant HBB allele and sickle cell anemia? - Answer-option 3- 2 mutant genes on chromosome 11
Which pair of chromosomes accurately depicts the genotype of a person with an X-linked recessive
mutant ALAS2 allele and sideroblastic anemia? - Answer-option 3, mutated allele
Studies have shown that increased expression of two genes called SIRT1 and SIRT2 is associated with
chronic exposure to cocaine and contributes to drug addiction.
What difference in the regions of the SIRT1 and SIRT2 genes in people addicted to cocaine increases
their expression? - Answer-The nucleosomes become more widely spaced.
Mice with low levels of agouti gene expression tend to have yellow fur and are obese, while mice with
higher levels of expression are brown and of normal weight. Studies show that expression of the agouti
gene in mice can be controlled by epigenetics.
and Answers
Which mRNA sequence results in a polypeptide that contains three amino acids? - Answer-5' - CUU GAU
ACU UAG - 3'
Which RNA sequence would end in the following polypeptide?
Phe-Thr-Val-Stop - Answer-5' - UUC ACA GUA UAA - 3'
What is the complementary sequence for the following DNA sequence?
5' - GCC ATC TCG AAT - 3' - Answer-5' - ATT CGA GAT GGC - 3'
Assuming 100% reaction efficiency, how many DNA copies are created after the completion of four
complete PCR cycles? - Answer-16
What is the function of DNA polymerase in the process of PCR? - Answer-It recognizes the primers and
uses the available dNTPs to replicate the template DNA sequence.
, 5' - [...] ATT CGC TAG GGC [...] - 3' − Normal DNA
5' - [...] ATT CGC GAG GGC [...] - 3' − Mutant DNA
Which type of mutation and outcome characterizes the Mutant DNA? - Answer-A missense mutation
leading to a longer protein
In sickle-cell disease, the glutamic acid (Glu) at position number seven is mutated to valine (Val).
What change in codons corresponds to this mutation? - Answer-GAA to GTA
What are possible blood types of the parents of a patient with blood type AB? - Answer-A and B
Which pair of chromosomes accurately depicts the genotype of a person with an autosomal recessive
mutant HBB allele and sickle cell anemia? - Answer-option 3- 2 mutant genes on chromosome 11
Which pair of chromosomes accurately depicts the genotype of a person with an X-linked recessive
mutant ALAS2 allele and sideroblastic anemia? - Answer-option 3, mutated allele
Studies have shown that increased expression of two genes called SIRT1 and SIRT2 is associated with
chronic exposure to cocaine and contributes to drug addiction.
What difference in the regions of the SIRT1 and SIRT2 genes in people addicted to cocaine increases
their expression? - Answer-The nucleosomes become more widely spaced.
Mice with low levels of agouti gene expression tend to have yellow fur and are obese, while mice with
higher levels of expression are brown and of normal weight. Studies show that expression of the agouti
gene in mice can be controlled by epigenetics.
