BIOSAFETY AND BIOSECURITY Test 2|
Questions with Answers Verified 100%
Correct
The classical definition of HEPA Filter is 99.97% at 0.3 Microns but nowadays BSC and LF use
___________________. - ✔✔99.99% at 0.3 µm.
REMOVES A BROAD RANGE
OF AIRBORNE
CONTAMINANTS:
-FINE DUST
-SMOKE
-BACTERIA (TYPICAL SIZE: 500
TO 0.3ΜM
-SOOT
-POLLEN
-RADIOACTIVE PARTICLES
-IMPURITY ION →CAN AFFECT
INTEGRATED CIRCUIT SPEED - ✔✔HEPA/ ULPA Capability
The distance of the two BSC should be ________________.(In what meter or feet apart) -
✔✔10ft. or 3m apart.
ANNUAL BSC TESTING includes: - ✔✔• HEPA FILTER LEAK TEST
,• INFLOW VELOCITY TEST
• DOWNFLOW VELOCITY TEST
• AIRFLOW PATTERN TEST
□ CONTRIBUTES TO WORKER PROTECTION
□ PROTECTS OUTSIDE THE LABORATORY - ✔✔SECONDARY BARRIERS /
ENGINEERING CONTROLS
Examples of Secondary Barriers? - ✔✔EXAMPLE:
□ BUILDING AND LAB DESIGN
□ VENTILATION
□ AUTOCLAVE
□ CAGE WASH FACILITIES
□ INCREASING LEVELS OF EMPLOYEE AND
ENVIRONMENTAL PROTECTION
□ GUIDELINES FOR WORKING SAFELY IN RESEARCH
AND CLINICAL LABORATORY - ✔✔BIOSAFETY
LEVELS
ASSIGNMENT OF MICROORGANISMS BASED ON:
(P,Mt,Hr,PM,T) - ✔✔□ PATHOGENICITY
□ MODE OF TRANSMISSION AND HOST RANGE
□ LOCAL AVAILABILITY OF EFFECTIVE PREVENTATIVE MEASURES
□ LOCAL AVAILABILITY OF EFFECTIVE TREATMENT
A microorganism that is unlikely to cause human or
animal disease.
, BSL-1
Typical Work Area- Open Bench
Example: S. cerevisiae (yeast), Lactobacillus, B. subtilis - ✔✔Risk Group 1
A pathogen that can cause human or animal
disease but is unlikely to be a serious hazard to
laboratory workers, the community, livestock or the
environment.
Laboratory exposures may cause serious infection, but
effective treatment and preventive measures are
available and the risk of spread of infection is limited.
BSL-2
Typical Work Area-Biosafety cabinet / laminar flow hood
Example Hepatitis B virus, HIV, the salmonellae and
Toxoplasma spp. - ✔✔Risk Group 2
A pathogen that usually causes serious human or
animal disease but does not ordinarily spread
from one infected individual to another. Effective
treatment and preventive measures are
available. BSL- 3
Typical Work Area : Class 3 biosafety cabinet
Example: M. tuberculosis, St. Louis encephalitis virus and Coxiella burnetti, Yersinia pestis, SARS
virus - ✔✔Risk Group 3
A pathogen that usually causes serious human or
animal disease and that can be readily
Questions with Answers Verified 100%
Correct
The classical definition of HEPA Filter is 99.97% at 0.3 Microns but nowadays BSC and LF use
___________________. - ✔✔99.99% at 0.3 µm.
REMOVES A BROAD RANGE
OF AIRBORNE
CONTAMINANTS:
-FINE DUST
-SMOKE
-BACTERIA (TYPICAL SIZE: 500
TO 0.3ΜM
-SOOT
-POLLEN
-RADIOACTIVE PARTICLES
-IMPURITY ION →CAN AFFECT
INTEGRATED CIRCUIT SPEED - ✔✔HEPA/ ULPA Capability
The distance of the two BSC should be ________________.(In what meter or feet apart) -
✔✔10ft. or 3m apart.
ANNUAL BSC TESTING includes: - ✔✔• HEPA FILTER LEAK TEST
,• INFLOW VELOCITY TEST
• DOWNFLOW VELOCITY TEST
• AIRFLOW PATTERN TEST
□ CONTRIBUTES TO WORKER PROTECTION
□ PROTECTS OUTSIDE THE LABORATORY - ✔✔SECONDARY BARRIERS /
ENGINEERING CONTROLS
Examples of Secondary Barriers? - ✔✔EXAMPLE:
□ BUILDING AND LAB DESIGN
□ VENTILATION
□ AUTOCLAVE
□ CAGE WASH FACILITIES
□ INCREASING LEVELS OF EMPLOYEE AND
ENVIRONMENTAL PROTECTION
□ GUIDELINES FOR WORKING SAFELY IN RESEARCH
AND CLINICAL LABORATORY - ✔✔BIOSAFETY
LEVELS
ASSIGNMENT OF MICROORGANISMS BASED ON:
(P,Mt,Hr,PM,T) - ✔✔□ PATHOGENICITY
□ MODE OF TRANSMISSION AND HOST RANGE
□ LOCAL AVAILABILITY OF EFFECTIVE PREVENTATIVE MEASURES
□ LOCAL AVAILABILITY OF EFFECTIVE TREATMENT
A microorganism that is unlikely to cause human or
animal disease.
, BSL-1
Typical Work Area- Open Bench
Example: S. cerevisiae (yeast), Lactobacillus, B. subtilis - ✔✔Risk Group 1
A pathogen that can cause human or animal
disease but is unlikely to be a serious hazard to
laboratory workers, the community, livestock or the
environment.
Laboratory exposures may cause serious infection, but
effective treatment and preventive measures are
available and the risk of spread of infection is limited.
BSL-2
Typical Work Area-Biosafety cabinet / laminar flow hood
Example Hepatitis B virus, HIV, the salmonellae and
Toxoplasma spp. - ✔✔Risk Group 2
A pathogen that usually causes serious human or
animal disease but does not ordinarily spread
from one infected individual to another. Effective
treatment and preventive measures are
available. BSL- 3
Typical Work Area : Class 3 biosafety cabinet
Example: M. tuberculosis, St. Louis encephalitis virus and Coxiella burnetti, Yersinia pestis, SARS
virus - ✔✔Risk Group 3
A pathogen that usually causes serious human or
animal disease and that can be readily
