Biology 171 Final Exam|41 Complete Q’s and
A’s
Griffith - -dead "S" cells transform living "R" cells
- A, M, & McCarty - -determined that DNA was needed for transformations
- Hershey & Chase - -used 35S to label proteins, and 32P to label DNA,
followed the infection of E. coli cells by phage viruses (DNA, not protein) is
passed on
- Chargoff - -A/T and G/C have ratios of 1:1
- Watson & Crick - -assembled information, proposed double helix model
- Franklin & Wilkins - -produced images of DNA using a technique called X-
ray crystallography (determined the shape and size of DNA)
- Helicase - -unwinds the double helix into separate strands
- Topoisomerase - -removes the supercoils that form ahead of the
replication fork
- Primase - -synthesizes a short RNA primer on the template strand
- DNA Polymerase III - -synthesizes the new strands at the replication fork,
uses dNTPs as substrates
- DNA Polymerase I - -removes the RNA primers, and replaces them with
DNA
- DNA Ligase - -adds the missing covalent bond between okazaki fragments
(seals the nicks)
- Single Stranded Binding Proteins - -bind single-stranded DNA and prevent
helix from reforming
- Beta clamp - -binds DNA polymerase and prevents the enzyme from
dissociating from the DNA template strand
- Aminoacyl tRNA Synthase - -adds amino acids to the correct tRNAs
- Promoter - -where RNA polymerase binds to initiate transcription
- RNA coding region - -elongation; RNA is made from DNA template
A’s
Griffith - -dead "S" cells transform living "R" cells
- A, M, & McCarty - -determined that DNA was needed for transformations
- Hershey & Chase - -used 35S to label proteins, and 32P to label DNA,
followed the infection of E. coli cells by phage viruses (DNA, not protein) is
passed on
- Chargoff - -A/T and G/C have ratios of 1:1
- Watson & Crick - -assembled information, proposed double helix model
- Franklin & Wilkins - -produced images of DNA using a technique called X-
ray crystallography (determined the shape and size of DNA)
- Helicase - -unwinds the double helix into separate strands
- Topoisomerase - -removes the supercoils that form ahead of the
replication fork
- Primase - -synthesizes a short RNA primer on the template strand
- DNA Polymerase III - -synthesizes the new strands at the replication fork,
uses dNTPs as substrates
- DNA Polymerase I - -removes the RNA primers, and replaces them with
DNA
- DNA Ligase - -adds the missing covalent bond between okazaki fragments
(seals the nicks)
- Single Stranded Binding Proteins - -bind single-stranded DNA and prevent
helix from reforming
- Beta clamp - -binds DNA polymerase and prevents the enzyme from
dissociating from the DNA template strand
- Aminoacyl tRNA Synthase - -adds amino acids to the correct tRNAs
- Promoter - -where RNA polymerase binds to initiate transcription
- RNA coding region - -elongation; RNA is made from DNA template
