MIC224 Final- Howard Test With
Complete Solution
Kirby- Bauer - ANSWER what is another name for the antibiotic sensitivity
test?
minimum inhibitory cone - ANSWER what does MIC stand for
the minimum amount that will kill the antibiotic - ANSWER what does the
MIC mean?
it did not kill the abx - ANSWER if it is growing what does it mean
it did kill the abx - ANSWER if it is not growing what does it mean
Mueller- Hinton Agar - ANSWER which agar is used for the antibiotic
sensitivity test
we will have to measure the zone of clearing and then compare to the chart
to decide if it is sensitive, intermediate, or resistant - ANSWER
**UNDERSTAND THE ZONE OF INHIBITION EVALUATION TABLE** PG. 50
mannitol - ANSWER what is the substrate for Mannitol Salt Agar (MSA)
phenol red - ANSWER what is the indicator for Mannitol Salt Agar (MSA)
NaCl - ANSWER what is the selective agent for Mannitol Salt Agar (MSA)
-yellow
-s. auereus
-fermented - ANSWER -what is the positive result of Mannitol Salt Agar
,(MSA)
-what is the name of this bacteria
-has it been fermented or respired
-red
-s. epidermis
-respired - ANSWER -what is the negative result of Mannitol Salt Agar (MSA)
-what is the name of this bacteria
-has it been fermented or respired
-yes
-S. aureus - ANSWER This is a MSA plate.
-Did this organism ferment or not?
-what organism is it?
-no
-S. epidermis - ANSWER **DO NOT HAVE PIC OF* If there was a MSA plate
and it was red.
-Did this organism ferment or not?
-what organism is it?
starch - ANSWER what is the substrate for Starch Agar (STA)
iodine - ANSWER what is the indicator for Starch Agar (STA)
none - ANSWER what is the selective agent for STA
, the presence of amylase - ANSWER what does a positive STA indicate
amylase - ANSWER the organism needs ___ to break down starch, so there is
no starch there
will see a zone of clearing - ANSWER how do you indicate a positive STA
no zone of clearing - ANSWER how do you indicate a negative STA
-yes
-amylase
-positive - ANSWER this is a STA plate
-is the zone of clearing present?
-what enzyme did it produce?
-negative or positive?
-no
-none
-negative - ANSWER this is a STA plate
-is the zone of clearing present?
-what enzyme did it produce?
-negative or positive?
gelatinase - ANSWER what is the substrate for GEL
HCl - ANSWER what is the indicator for GEL
none - ANSWER what is the selective agent for GEL
Complete Solution
Kirby- Bauer - ANSWER what is another name for the antibiotic sensitivity
test?
minimum inhibitory cone - ANSWER what does MIC stand for
the minimum amount that will kill the antibiotic - ANSWER what does the
MIC mean?
it did not kill the abx - ANSWER if it is growing what does it mean
it did kill the abx - ANSWER if it is not growing what does it mean
Mueller- Hinton Agar - ANSWER which agar is used for the antibiotic
sensitivity test
we will have to measure the zone of clearing and then compare to the chart
to decide if it is sensitive, intermediate, or resistant - ANSWER
**UNDERSTAND THE ZONE OF INHIBITION EVALUATION TABLE** PG. 50
mannitol - ANSWER what is the substrate for Mannitol Salt Agar (MSA)
phenol red - ANSWER what is the indicator for Mannitol Salt Agar (MSA)
NaCl - ANSWER what is the selective agent for Mannitol Salt Agar (MSA)
-yellow
-s. auereus
-fermented - ANSWER -what is the positive result of Mannitol Salt Agar
,(MSA)
-what is the name of this bacteria
-has it been fermented or respired
-red
-s. epidermis
-respired - ANSWER -what is the negative result of Mannitol Salt Agar (MSA)
-what is the name of this bacteria
-has it been fermented or respired
-yes
-S. aureus - ANSWER This is a MSA plate.
-Did this organism ferment or not?
-what organism is it?
-no
-S. epidermis - ANSWER **DO NOT HAVE PIC OF* If there was a MSA plate
and it was red.
-Did this organism ferment or not?
-what organism is it?
starch - ANSWER what is the substrate for Starch Agar (STA)
iodine - ANSWER what is the indicator for Starch Agar (STA)
none - ANSWER what is the selective agent for STA
, the presence of amylase - ANSWER what does a positive STA indicate
amylase - ANSWER the organism needs ___ to break down starch, so there is
no starch there
will see a zone of clearing - ANSWER how do you indicate a positive STA
no zone of clearing - ANSWER how do you indicate a negative STA
-yes
-amylase
-positive - ANSWER this is a STA plate
-is the zone of clearing present?
-what enzyme did it produce?
-negative or positive?
-no
-none
-negative - ANSWER this is a STA plate
-is the zone of clearing present?
-what enzyme did it produce?
-negative or positive?
gelatinase - ANSWER what is the substrate for GEL
HCl - ANSWER what is the indicator for GEL
none - ANSWER what is the selective agent for GEL
